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Cat. No.: HY-A0083B

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Methacholine bromide

(Synonyms: Acetyl-β-methylcholine bromide)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Methacholine (Acetyl-β-methylcholine) bromide is a potent muscarinic-3 (M3) agonist. Methacholine bromide acts directly on acetylcholine receptors on smooth muscle causing bronchoconstriction and airway narrowing. Methacholine bromide shows a high sensitivity to identify bronchial hyperresponsiveness (BHR). Methacholine bromide can be used to measure airway hyperresponsiveness (AHR) as a diagnostic aid in the assessment of individuals with asthma-like symptoms and normal resting expiratory flow rates.

For research use only. We do not sell to patients.

CAS No.: 333-31-3
Formula: C₈H₁₈BrNO₂
Molecular Weight:240.14

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (270 KB)

Methacholine bromide Chemical Structure

CAS No.: 333-31-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vivo
Chemical Information
Publications (6)
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All mAChR Isoforms :

In Vivo

Methacholine bromide (0.5 µg/kg plus 5 µg/kg/min for 30 min) induces bronchoconstriction in dogs^[4].
Methacholine bromide (0.5 mg/kg; i.v.) induces bronchoconstriction was inhibited by bradykinin (4-40 µg/kg; i.v.) in a a dose-dependent manner in mouse^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

9-week female BALB/c mice^[6]
Dosage:

0.03, 0.1, 0.3, 1 mg/kg
Administration:

I.v
Result:

Induced severe bronchoconstriction.

Chemical Information

CAS No. 333-31-3
Molecular Weight 240.14
Formula C₈H₁₈BrNO₂
SMILES

CC(OC(C)=O)C[N+](C)(C)C.[Br-]
Synonyms

Acetyl-β-methylcholine bromide
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (6)

All In Vivo Efficacy Study

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Cell Death Dis

E3 Ubiquitin ligases Cbl-b and c-Cbl maintain the homeostasis of macrophages by regulating the M-CSF/M-CSFR signaling axis. [Abstract]

2025 Oct 7;16(1):716. PMID: 41057360

Methacholine bromide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Oct 7;16(1):716. [Abstract]

Methacholine chloride (6.25-50 mg/mL; i.n.; 5 min). The breathing frequency (f), inspiratory time (Ti), expiratory time (Te), tidal volume, baseline (TVb), and expiratory end pressure (EEP) of dKO mice were measured by the WBP system.
J Ethnopharmacol

YiQi GuBen formula targets EHMT2 and enhances NEDD4L transcription to combat epithelial-mesenchymal transition and airway remodeling in asthma. [Abstract]

2026 Mar 25:359:121125. PMID: 41475628

Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]

AHR of OVA-sensitized BALB/c mice with or without YQGBF treatment was examined via Methacholine chloride (10-30 mg/mL; 3 min) exposure.

Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]

AHR of OVA-sensitized BALB/c mice with or without EHMT2 overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]

AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and NEDD4L overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.

Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]

AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and treated with Wnt/β-catenin inhibitor ICG-001 was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.
J Ethnopharmacol

Network pharmacology and experimental validation identify the targets of Marsdenia tenacissima in neutrophilic asthma treatment. [Abstract]

2026 Feb 10:356:120782. PMID: 41135618
Naunyn Schmiedebergs Arch Pharmacol

Lactobacillus rhamnosus regulates airway epithelial cell senescence through the ADCK5/PI3K/AKT signaling axis and alleviates airway inflammation in asthma. [Abstract]

2025 Nov 12. PMID: 41222693
Immunobiology

Exosomes derived from baicalin-pretreated bone marrow mesenchymal stem cells inactivate the TLR4/MyD88/NF-kB pathway to improve asthma. [Abstract]

2025 Jul 16;230(4):153103. PMID: 40695068
Aging (Albany NY)

Cytokine antibody array-based analysis of IL-37 treatment effects in asthma. [Abstract]

2021 Sep 13;13(17):21729-21742. PMID: 34516405

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Cohen J, et al. Relationship between airway responsiveness to neurokinin A and methacholine in asthma. Pulm Pharmacol Ther. 2005;18(3):171-176. [Content Brief]

[2]. Anderson SD, et al. Comparison of mannitol and methacholine to predict exercise-induced bronchoconstriction and a clinical diagnosis of asthma. Respir Res. 2009;10(1):4. Published 2009 Jan 23. [Content Brief]

[3]. Cockcroft DW. Methacholine challenge methods. Chest. 2008;134(4):678-680. [Content Brief]

[4]. Kabara S, et al. Differential effects of thiopental on methacholine- and serotonin-induced bronchoconstriction in dogs. Br J Anaesth. 2003 Sep;91(3):379-84. [Content Brief]

[5]. Folkerts G, et al. Bradykinin causes inhibition of methacholine-induced bronchoconstriction in vivo in mice. Naunyn Schmiedebergs Arch Pharmacol. 2001 Jul;364(1):53-8. [Content Brief]

[6]. Vitorasso RL, et al. Methacholine dose response curve and acceptability criteria of respiratory mechanics modeling. Exp Lung Res. 2020 Feb-Mar;46(1-2):23-31. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Methacholine bromide

6 Cited Publications

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Biological Activity
- Description
- In Vivo
Chemical Information
Publications (6)
Purity & Documentation
References

1/16
1/16

Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]

Customer Validation
AHR of OVA-sensitized BALB/c mice with or without YQGBF treatment was examined via Methacholine chloride (10-30 mg/mL; 3 min) exposure.
1/16

Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]

Customer Validation
AHR of OVA-sensitized BALB/c mice with or without EHMT2 overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.
1/16

Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]

Customer Validation
AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and NEDD4L overexpression was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.
1/16

Methacholine bromide purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2026 Mar 25:359:121125. [Abstract]

Customer Validation
AHR of OVA-sensitized BALB/c mice with EHMT2 overexpression and treated with Wnt/β-catenin inhibitor ICG-001 was examined via Methacholine chloride (10–30 mg/mL; 3 min) exposure.
1/16

Methacholine bromide purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2025 Oct 7;16(1):716. [Abstract]

Customer Validation
Methacholine chloride (6.25-50 mg/mL; i.n.; 5 min). The breathing frequency (f), inspiratory time (Ti), expiratory time (Te), tidal volume, baseline (TVb), and expiratory end pressure (EEP) of dKO mice were measured by the WBP system.

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Methacholine bromide

Publications Citing Use of MedChemExpress (MCE) Methacholine bromide

All mAChR Isoforms

Biological Activity

Chemical Information

Publications (6)

Purity & Documentation

References

Calculators

Help & FAQs

Methacholine bromide Related Classifications

HY-A0083B Related Classifications