DMT-dT Phosphoramidite
Based on 1 Customer Validation
DMT-dT Phosphoramidite is typically used in the synthesis of DNA.
For research use only. We do not sell to patients.
- Purity: 98.83%
- CAS No.: 98796-51-1
- Formula: C40H49N4O8P
- Molecular Weight:744.81
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
DMT-dT Phosphoramidite is used as a protecting group and synthetic intermediate in DNA synthesis. DMT (Dimethoxytrityl) is protecting group for protecting the hydroxyl (-OH) group in DNA synthesis from undergoing unwanted reactions. dT stands for deoxythymidine, which is one of the basic building blocks of DNA. Phosphoramidite interacts with the hydroxyl group (-OH) at the 5' end of the DNA chain to form a new phosphodiester bond, thereby extending the DNA chain. DMT-dT Phosphoramidite can be used for surface functionalization or DNA probe synthesis[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 98796-51-1
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Appearance Solid
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Molecular Weight 744.81
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Formula C40H49N4O8P
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Color White to off-white
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SMILES
COC(C=C1)=CC=C1C(OC[C@@H]2[C@@H](OP(OCCC#N)N(C(C)C)C(C)C)C[C@H](N3C(NC(C(C)=C3)=O)=O)O2)(C4=CC=C(OC)C=C4)C5=CC=CC=C5
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 200 mg/mL (268.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (2.79 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3426 mL | 6.7131 mL | 13.4262 mL | 33.5656 mL |
| 5 mM | 0.2685 mL | 1.3426 mL | 2.6852 mL | 6.7131 mL | |
| 10 mM | 0.1343 mL | 0.6713 mL | 1.3426 mL | 3.3566 mL | |
| 15 mM | 0.0895 mL | 0.4475 mL | 0.8951 mL | 2.2377 mL | |
| 20 mM | 0.0671 mL | 0.3357 mL | 0.6713 mL | 1.6783 mL | |
| 25 mM | 0.0537 mL | 0.2685 mL | 0.5370 mL | 1.3426 mL | |
| 30 mM | 0.0448 mL | 0.2238 mL | 0.4475 mL | 1.1189 mL | |
| 40 mM | 0.0336 mL | 0.1678 mL | 0.3357 mL | 0.8391 mL | |
| 50 mM | 0.0269 mL | 0.1343 mL | 0.2685 mL | 0.6713 mL | |
| 60 mM | 0.0224 mL | 0.1119 mL | 0.2238 mL | 0.5594 mL | |
| 80 mM | 0.0168 mL | 0.0839 mL | 0.1678 mL | 0.4196 mL | |
| 100 mM | 0.0134 mL | 0.0671 mL | 0.1343 mL | 0.3357 mL |
DMT-dT Phosphoramidite Related Classifications
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