2. Cell Cycle/DNA Damage Apoptosis Epigenetics Anti-infection
  3. Topoisomerase Caspase PARP Fungal DNA/RNA Synthesis
  4. Echinoside A

Echinoside A is a saponin. Echinoside A can be isolated from sea cucumber. Echinoside A inhibits the catalytic activity of Top2α, reduces the noncovalent binding of Top2α to DNA. Echinoside A activates Caspase-3 and induces PARP cleavage. Echinoside A induces Apoptosis. Echinoside A has anticancer activity against prostate cancer, hepatocellular carcinoma, and S-180 sarcoma. Echinoside A exhibits antifungal activity against a variety of fungi, with a minimum growth inhibitory concentration range of 3.12 to 50.0 μg/mL, including potent activity against Aspergillus and Penicillium species.

For research use only. We do not sell to patients.

Echinoside A

Echinoside A Chemical Structure

CAS No. : 75410-53-6

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Echinoside A Related Antibodies

Top Publications Citing Use of Products

View All Topoisomerase Isoform Specific Products:

View All Isoforms
Topoisomerase Topo I Topo II DNA gyrase

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All PARP Isoform Specific Products:

View All Isoforms
PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

View All DNA/RNA Synthesis Isoform Specific Products:

View All Isoforms
RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Echinoside A is a saponin. Echinoside A can be isolated from sea cucumber. Echinoside A inhibits the catalytic activity of Top2α, reduces the noncovalent binding of Top2α to DNA. Echinoside A activates Caspase-3 and induces PARP cleavage. Echinoside A induces Apoptosis. Echinoside A has anticancer activity against prostate cancer, hepatocellular carcinoma, and S-180 sarcoma. Echinoside A exhibits antifungal activity against a variety of fungi, with a minimum growth inhibitory concentration range of 3.12 to 50.0 μg/mL, including potent activity against Aspergillus and Penicillium species[1][2][3].

IC50 & Target[1]

topoisomerase II alpha

 

Caspase-3

 

In Vitro

Echinoside A (0.80-3.98 μM; 6-24 h) potently inhibits the proliferation of HepG2 cells in a dose-dependent manner, and reduces cell viability by 54.7% at 2.70 μM for 12 h[1].
Echinoside A (1.35-2.70 μM; 12 h) induces apoptosis in HepG2 cells in a dose-dependent manner in vitro[1].
Echinoside A (1.35-2.70 μM; 12 h) regulates the expression of cell cycle-related genes in HepG2 cells, upregulates the expression of p16 and p21, downregulates the expression of cyclin D1, but has no effect on p27[1].
Echinoside A (1.35-2.70 μM; 12 h) exerts no significant effect on the expression of NF-κB p65 protein in HepG2 cells[1].
Echinoside A (0.25-2 μM; 3 h for DNA fragmentation) induces concentration-dependent apoptosis and DNA fragmentation in human leukemia HL-60 cells[2].
Echinoside A exhibits antifungal activity against a variety of fungi, with a minimum growth inhibitory concentration range of 3.12 to 50.0 μg/mL, including potent activity against *Aspergillus* and *Penicillium* species[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Echinoside A Related Antibodies

Apoptosis Analysis[1]

Cell Line: human hepatocellular liver carcinoma HepG2 cells
Concentration: 1.35, 2.70 μM
Incubation Time: 12 h
Result: Showed slightly increased percentages of early and late apoptotic cells compared to control cells.
Induced visible perinuclear chromatin condensation as bright green patches or fragments in cells treated with 2.70 μM.\nResulted in 18.1% total apoptotic cells (13.2% early apoptotic, 4.9% late apoptotic) at 1.35 μM for 12 h.
Resulted in 62.2% total apoptotic cells at 2.70 μM for 12 h.
Induced apoptosis in a dose-dependent manner.

Cell Cycle Analysis[1]

Cell Line: human hepatocellular liver carcinoma HepG2 cells
Concentration: 1.35, 2.70 μM
Incubation Time: 12 h
Result: Increased the population of HepG2 cells in the G0/G1 phase from 67.9% to 75.2% at 2.70 μM for 12 h.
Increased the sub-G0/G1 cell population (indicative of apoptosis) from 1.83% to 21.21% at 2.70 μM for 12 h.

Western Blot Analysis[1]

Cell Line: human hepatocellular liver carcinoma HepG2 cells
Concentration: 1.35, 2.70 μM
Incubation Time: 12 h
Result: Activated caspase-3 and induced PARP cleavage.\nHad no significant inhibitory effect on NF-κB p65 protein expression.
In Vivo

Echinoside A (0.5-2.5 mg/kg; i.p.; daily; 10 days) inhibits hepatocellular carcinoma tumour growth in a dose-dependent manner in BALB/c mice, with a 33.8% inhibition rate at 0.5 mg/kg and a 49.8% inhibition rate at 2.5 mg/kg[1].
Echinoside A (1.5-3.0 mg/kg/day; i.v.; daily; 7 days) reduces S-180 sarcoma growth in KM mice[2].
Echinoside A (2.6-5.2 mg/kg; i.v.; weekly; 4 weeks) suppresses PC-3 prostate carcinoma xenograft growth in nude mice with T/C % values of 53.7% and 45.1%, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (male, 17-22g, subcutaneous implantation of H22 hepatocarcinoma cells)[1]
Dosage: 0.5 mg/kg; 2.5 mg/kg
Administration: i.p.; daily; 10 days
Result: Reduced mean tumour weight to 1.53 ± 0.36 g, corresponding to a 33.8% tumour growth inhibition rate (P < 0.01).
Reduced mean tumour weight to 1.16 ± 0.22 g, corresponding to a 49.8% tumour growth inhibition rate (P < 0.01).
Animal Model: KM mice (female, 7 weeks old, 18-22 g, specific pathogen-free, S-180 sarcoma model via s.c. inoculation)[2]
Dosage: 1.5 mg/kg/day; 3.0 mg/kg/day
Administration: i.v.; daily; 7 days
Result: Achieved a tumor growth inhibition rate of 52.0% at 1.5 mg/kg.
Achieved a tumor growth inhibition rate of 80.0% at 3 mg/kg.
Molecular Weight

1207.31

Formula

C54H87NaO26S
CAS No.
SMILES

C[C@]12[C@]3([C@H](C=C4[C@@]2([H])CC[C@]5([H])[C@@]4(CC[C@@H](C5(C)C)O[C@H]6[C@@H]([C@H]([C@@H](CO6)OS(=O)(O[Na])=O)O)O[C@H]7[C@@H]([C@H]([C@@H]([C@H](O7)C)O[C@H]8[C@@H]([C@H]([C@@H]([C@H](O8)CO)O)O[C@H]9[C@@H]([C@H]([C@@H]([C@H](O9)CO)O)OC)O)O)O)O)C)O)[C@@]([C@@](C)(OC3=O)CCCC(C)C)(CC1)O
Structure Classification
Initial Source

Echinoside A was isolated from sea cucumber
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Echinoside A Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Echinoside A75410-53-6TopoisomeraseCaspasePARPFungalDNA/RNA Synthesispoly ADP ribose polymerasehepatocarcinomaDNA double-strand breaksmitochondrial pathwayP-glycoproteinHepG2 cellsG0/G1 phasetopoisomerase 2αNa+K+-adenosine triphosphatasexenograft modelsHL-60 human leukemia cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Echinoside A
Cat. No.:
HY-N17442
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.