Biochemical Detection Enzymes

Alanine aminotransferase, Porcine heart is a pyridoxal enzyme that catalyzes the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate.

Uricase, Microorganism (Uox, Microorganism) is a uricase (urate oxidase) derived from Microorganism. Uricase, Microorganism converts uric acid into allantoin. The absence of Uricase in mammals causes kidney diseases resulting from uric acid accumulation. Uricase, Microorganism can be used for research on chronic refractory gout and hyperuricemia.

3α-Hydroxysteroid Dehydrogenase, Microorganism (3α-HSD) is an enzyme encoded by the AKR1C4 gene, which can catalyze the conversion of 3-ketosteroids into 3α-hydroxy compounds. 3α-Hydroxysteroid dehydrogenase plays an important role in the inactivation of androgen DHT, and can convert DHT into 3α-androstanediol with weak androgen activity, which can be used in the research of hirsutism.

Mutanolysin is a bacteriolytic agent. Mutanolysin is a muralytic enzyme that can prevent hepatic injury. Mutanolysin can digest the cell wall of S. mutans BHT and shows antibacterial activity. Mutanolysin reduces TNF-α production in isolated Kupffer cells stimulated with peptidoglycan-polysaccharide (PG-APS). Mutanolysin can be used for the researches of infection, inflammation and hepatic injury.

Bacterial α-Amylase catalyses the hydrolysis of internal α-1,4-glycosidic linkages in starch in low molecular weight products, such glucose, maltose and maltotriose units. Bacterial α-Amylase is often used in biochemical studies.

L-Lactate Dehydrogenase, rabbit muscle is a biological material or organic compound that can be used in life science research.

Phosphodiesterase I (PDE) is an enzyme that can catalyze the hydrolysis of the 3' ring phosphate bond of cyclic nucleotides, and is often used in biochemical research. Phosphodiesterase I acts as an important regulator of signal transduction mediated by the second messenger molecules cAMP and cGMP. According to their specificity to cyclic nucleotides, they can also be divided into different types, such as PDE1-PDE11, which also have certain potential in various diseases.

Malate dehydrogenase (EC 1.1.1.37) (MDH) catalyzes the mutual conversion of oxaloacetate and malate, and is associated with the oxidation/reduction of dinucleotide coenzymes.

Glutamate dehydrogenase is an enzyme in both prokaryotes and eukaryotic mitochondria. Glutamate dehydrogenase can be used for the enzymatic determination of ammonia, alpha-ketoglutaric acid, L-glutamate and urease.

NAD(P)H-Nitrate reductase is isolated from Aspergillus niger that catalyses the reduction of nitrate to nitrite via a two-electron transfer. In plants, the electron donor for Nitrate reductase is NADPH is NADH:Nitrate reductase and a bispecific NAD(P)H: Nitrate reductase.

Chondroitinase ABC (ChABC) is an enzyme that degrades glycosaminoglycan side-chains of chondroitin sulfate (CS-GAG) from the chondroitin sulfate proteoglycan (CSPG) core protein. Chondroitinase ABC facilitates reinnervation by degrading CS-GAGs around motoneurons. Chondroitinase ABC has the potential for the research of spinal injury.

Sulfatase, Helix pomatia is an enzyme that catalyzes the hydrolysis of sulfate esters. Sulfatase, Helix pomatia participates in the metabolism of various substances.

Cholesterol esterase, Pseudomonas is an enzyme that hydrolyzes cholesterol ester to cholesterol and free fatty acid in the intestinal lumen. Cholesterol synthesized in the acinar cells and is stored in zymogen granules. Cholesterol esterase is also known as bile salt-stimulated lipase and carboxy ester lipasea, acts function for acceleration of cholesterol absorption.

Glutamate dehydrogenase (NAD (P)) (GLDH) is a glutamate dehydrogenase. Glutamate dehydrogenase exists in all organisms and catalyzes the oxidative deamination of L-Glutamate (HY-W337739) to α-Ketoglutarate using NAD (P)⁺ as a coenzyme. The glutamate dehydrogenase pathway is also associated with various cellular processes, including ammonia metabolism, acid-base balance, redox homeostasis, lipid biosynthesis, and lactate production.

Purine nucleoside phosphorylase, Microorganism (PNP) is a key enzyme in purine metabolism, which is involved in the purine rescue pathway. The deficiency of Purine nucleoside phosphorylase resulted in impaired T cell function. In the presence of inorganic orthophosphate as the second substrate, Purine nucleoside phosphorylase catalyzes the breaking of the glycosidic bond between ribose and deoxyribonucleoside to generate purine base and ribose (deoxyribose) -1-phosphate.

Carbonic anhydrase, Bovine erythrocytes (EC 4.2.1.1) is ubiquitous zinc-containing metalloenzyme present in prokaryotes and eukaryotes. Carbonic anhydrase can catalyze reversible conversion of carbon dioxide to bicarbonate and protons. Carbonic anhydrase can be used for the research of cancer, glaucoma, obesity and epilepsy.

Glucose-6-phosphate dehydrogenase, Bacillus sp. (G6PD, Bacillus sp.) (EC 1.1.1.49) is an NADP-dependent antioxidant metabolic enzyme. Glucose-6-phosphate dehydrogenase, Bacillus sp. is induced by sublethal doses of Menadione (HY-B0332) and increasing concentrations of NaCl in Bacillus sp. F26. Glucose-6-phosphate dehydrogenase, Bacillus sp. catalyzes the oxidation of glucose-6-phosphate (G6P) to 6-phosphoglucono-δ-lactone, while reducing NADP⁺ to NADPH, providing reducing power for the antioxidant defense system and cellular redox balance of Bacillus sp. F26. Glucose-6-phosphate dehydrogenase, Bacillus sp. is used in research related to diabetic complications.

Oxalate Oxidase, or oxalate oxidase, catalyzes the oxidation of oxalic acid to hydrogen peroxide and carbon dioxide in the presence of oxygen. Oxalate Oxidase can be found in a variety of plants (such as barley) and microorganisms and can be used to treat wastewater and filtrates containing oxalic acid.

Glutathione Peroxidase (GSH-Px; EC 1.11.1.9) belongs to the peroxidase family and is commonly used in biochemical research. Glutathione Peroxidase can catalyze reduced glutathione (GSH) to form a disulfide bridge with another glutathione molecule, convert it into oxidized glutathione (GSSG), and react with hydrogen peroxide or lipid peroxide reaction, reducing it to H₂O. Glutathione Peroxidase is an effective antioxidant against oxidative stress.

Pyranose oxidase, Coriolus sp. is an oxidoreductase targeting monosaccharides (e.g., D-glucose, D-galactose). Pyranose oxidase, Coriolus sp. is promising for research of biosensors (glucose, 1,5-anhydroglucitol detection) and biofuel cells.