Chlorothalonil
Based on 1 publication(s) in Google Scholar
Chlorothalonil is a broad-spectrum foliar fungicide with oral activity. Chlorothalonil can be used to combat fungal diseases in vegetable and crop leaves. Chlorothalonil can alter the microbial community in the soil. Chlorothalonil inhibits spermatogenesis. Chlorothalonil can cause intestinal epithelial barrier dysfunction and fetal toxicity.
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- Pureza: 98.15%
- No. CAS: 1897-45-6
- Fòrmula: C8Cl4N2
- Peso molecular:265.91
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Chlorothalonil
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Actividad biológica
Chlorothalonil (10mg/kg soil) inhibits the activity of phosphatase and dehydrogenase activity in soil, reduces the number of microorganisms in soil[3].
Chlorothalonil (0.6-4.8 µg/mL; 4 days) induces intestinal epithelial barrier (IEB) dysfunction in Caco-2 cells by activating the mitogen-activated protein kinase (MAPK) pathway[4].
Chlorothalonil (0.1-10 μM; 4 h and 24 h) reduces porcine sperm motility and increases apoptosis in a concentration- and time-dependent manner[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Caco-2 (Human colorectal adenocarcinoma cell line)
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Concentration:0.6 µg/mL, 1.2 µg/mL , 2.4 µg/mL, 4.8 µg/mL
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Incubation Time:1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 8 days
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Result:Down-regulated the mRNA levels of TJ genes (ZO-1, OCLN, and CLDN1), polar marker genes (SI), and anti-apoptotic genes (BCL-2), and up-regulated the mRNA levels of apoptosis-related genes (BAD, BAX, CASP3, and CASP8).
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Cell Line:Caco-2 (Human colorectal adenocarcinoma cell line)
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Concentration:0.6 µg/mL, 1.2 µg/mL , 2.4 µg/mL
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Incubation Time:4 days
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Result:Decreased levels of the TJ protein network (ZO-1 and CLDN1).
Increased the expression levels of P-ERK1/2, PJNK and P-p38 and reduced levels of ERK1/2, JNK and p38.( All are mitogen-activated protein kinase (MAPK) signaling pathway-related proteins)
Chlorothalonil (0.1-10 mg/kg; p.o.; Once daily for 5 weeks) reduces sperm motility and inhibits spermatogenesis in ICR mice[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:ICR mouse model of pregnancy[1]
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Dosage:100 mg/kg, 400 mg/ kg, 600 mg/kg
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Administration:Oral gavage (p.o.); Once daily for 18 days
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Result:Reduced weight gain in pregnant mice at doses of 400 mg/kg and 600 mg/kg throughout pregnancy by 36% and 48%, respectively.
Significantly increased embryo mortality at doses of 400 mg/kg and 600 mg/kg.
Significantly reduced number of live births (28%).
Reduced mean fetal weight (22% to 39%) at doses of 400 and 600 mg/kg.
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Animal Model:ICR male mice model[5]
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Dosage:0.1mg/kg, 1 mg/ kg, 10 mg/kg
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Administration:Oral gavage (p.o.); Once daily for 5 weeks
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Result:Decreased sperm motility in ICR mice.
At doses of 1 mg/kg and 10 mg/kg, decreased the protein factors A-myb, GDNF and DDX4, and finally interrupted the spermatogenesis process.
Decreased the sperm marker Acrosin and PNA in a dose-dependent manner at doses of 1 mg/kg and 10 mg/kg.
At doses of 1 mg/kg and 10 mg/kg, Significantly reduced the number of estrogen receptor Alpha (ERα) positive stromal cells in mouse testis.
Decreased AKT levels in the testis, but did not change p-AKT levels.
Disrupted the methylation of histones and DNA.
Chemical Information
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No. CAS 1897-45-6
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Appearance Solid
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Peso molecular 265.91
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Fòrmula C8Cl4N2
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Color White to off-white
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SMILES
N#CC1=C(Cl)C(Cl)=C(Cl)C(C#N)=C1Cl
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Ecotoxicol Environ Saf
Chlorothalonil exposure compromised mouse oocyte in vitro maturation through inducing oxidative stress and activating MAPK pathway. [Abstract]2024 Mar 15:273:116100. PMID: 38367607
Solvente y solubilidad
DMSO : 33.33 mg/mL (125.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (283 KB)
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SDS (949 KB)
- English - EN (949 KB)
- Français - FR (949 KB)
- Deutsch - DE (949 KB)
- Norwegian - NO (949 KB)
- Español - ES (949 KB)
- Swedish - SV (949 KB)
- Italian - IT (949 KB)
- Korean - KR (949 KB)
- Portuguese - PT (949 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Farag A T, et al. Embryotoxicity of oral administered chlorothalonil in mice[J]. Birth Defects Research Part B: Developmental and Reproductive Toxicology, 2006, 77(2): 104-109. [Content Brief]
[3]. Singh BK, et al. Degradation of chlorpyrifos, fenamiphos, and chlorothalonil alone and in combination and their effects on soil microbial activity. Environ Toxicol Chem. 2002 Dec;21(12):2600-5. PMID: 12463554. [Content Brief]
[4]. Tao H, et al. Chlorothalonil induces the intestinal epithelial barrier dysfunction in Caco-2 cell-based in vitro monolayer model by activating MAPK pathway. Acta Biochim Biophys Sin (Shanghai). 2021 Nov 10;53(11):1459-1468. [Content Brief]
[5]. Zhang P, et al. Low dose chlorothalonil impairs mouse spermatogenesis through the intertwining of Estrogen Receptor Pathways with histone and DNA methylation. Chemosphere. 2019 Sep;230:384-395. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7607 mL | 18.8034 mL | 37.6067 mL | 94.0168 mL |
| 5 mM | 0.7521 mL | 3.7607 mL | 7.5213 mL | 18.8034 mL | |
| 10 mM | 0.3761 mL | 1.8803 mL | 3.7607 mL | 9.4017 mL | |
| 15 mM | 0.2507 mL | 1.2536 mL | 2.5071 mL | 6.2678 mL | |
| 20 mM | 0.1880 mL | 0.9402 mL | 1.8803 mL | 4.7008 mL | |
| 25 mM | 0.1504 mL | 0.7521 mL | 1.5043 mL | 3.7607 mL | |
| 30 mM | 0.1254 mL | 0.6268 mL | 1.2536 mL | 3.1339 mL | |
| 40 mM | 0.0940 mL | 0.4701 mL | 0.9402 mL | 2.3504 mL | |
| 50 mM | 0.0752 mL | 0.3761 mL | 0.7521 mL | 1.8803 mL | |
| 60 mM | 0.0627 mL | 0.3134 mL | 0.6268 mL | 1.5669 mL | |
| 80 mM | 0.0470 mL | 0.2350 mL | 0.4701 mL | 1.1752 mL | |
| 100 mM | 0.0376 mL | 0.1880 mL | 0.3761 mL | 0.9402 mL |
Chlorothalonil Related Classifications
HY-N6625 Related Classifications
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- Chlorothalonil
- 1897-45-6
- Environmental Pollutants
- Fungal
- Estrogen Receptor/ERR
- Soil microorganisms
- Soil degradation rate
- Caco-2
- Estrogen receptor alpha (ERα)
- Histone methylation
- DNA methylation
- Intestinal epithelial barrier
- Mitogen-activated protein kinase (MAPK)
- Developmental toxicity
- Fungicide
- Inhibitor
- inhibitor
- inhibit