JS207
JS207 is a humanized IgG4-specific antibody targeting PD-1 and VEGFA. JS207 can block PD-1/PD-L1, PD-1/PD-L2, and VEGFA/VEGFR2 interactions, with IC50 values of 6.37 nM, 4.3 nM, and 3.35 nM, respectively. JS207 can relieve immunosuppression, promote T cell activation and IL-2/IFN-γ release, and induce PD-1 internalization. JS207 can inhibit VEGF-driven angiogenesis and has the property that VEGFA enhances PD-1 internalization. JS207 cross-reacts with cynomolgus monkey PD-1/VEGFA and rat/mouse VEGFA, but does not bind rat/mouse PD-1. JS207 shows antitumor efficacy in mouse colon cancer and melanoma models. JS207 can be used for research related to colon cancer and melanoma.
Para uso exclusivo en investigación. No vendemos a pacientes.
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Actividad biológica
Descripciòn
Species Reactivity
Human
In Vitro
JS207 binds human PD-1 with an EC50 of 10.4 ng/mL (58.6 pM) and is specific for related immune proteins[1].
JS207 cross-reacts with human and cynomolgus monkey PD-1 as well as human, cynomolgus monkey, rat, and mouse VEGFA[1].
JS207 (2 μg/mL) binds human PD-1 with a KD of 0.46 nM[1].
JS207 (30 nM; 0.5-4 h) induced PD-1 internalization in H293/PD-1 cells, reaching up to 65% in the presence of VEGFA[1].
JS207 (15 pM-150 nM; 5 days) promotes IL-2 and IFN-γ release in the MLR assay[1].
JS207 specifically binds to human VEGFA but does not bind to other human VEGF family proteins[1].
JS207 binds human VEGFA with a KD of 9 pM[1].
JS207 inhibits VEGFA/VEGFR2 signaling in the H293/VEGFR2 reporter gene assay with an IC50 of 0.773 nM[1].
JS207 (0.005-10 nM; 96 h) inhibits VEGFA-induced HUVEC proliferation with an IC50 of 0.296 nM[1].
JS207 can simultaneously bind to human PD-1 and human VEGFA[1].
JS207 blocks PD-1/PD-L1 in Jurkat/PD-1 and CHO/PD-L1 reporter gene assays with an EC50 of 2.89 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
JS207 (1.0-10.0 mg/kg; intraperitoneal injection; twice weekly; 6 doses total) produces dose-dependent tumor growth inhibition in the A375 melanoma NDG mouse model[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6-Pdcd1tm1(PDCD1)Bcgen/Bcgen (B-hPD-1 humanized mice)[1]
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Dosage:0.75 mg/kg, 1.5 mg/kg, and 4.5 mg/kg
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Administration:intraperitoneally; twice weekly; for 6 doses
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Result:Significantly inhibited tumor growth dose-dependently at 0.75, 1.5, and 4.5 mg/kg, achieving tumor growth inhibition (TGI) rates of 76.1%, 78.0%, and 84.4%, respectively, at day 20.
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Animal Model:NDG mice were implanted subcutaneously with A375 cells at 5×106 cells/mouse suspended and mixed with Matrigel[1]
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Dosage:1.0 mg/kg, 3.0 mg/kg, and 10.0 mg/kg
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Administration:intraperitoneally; twice weekly; for 6 doses
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Result:In the saline group, the mean tumor volume reached 585 mm3 at day 21.
At 1, 3, and 10 mg/kg, mean tumor volumes were 362 mm3, 344 mm3, and 262 mm3, corresponding to TGI rates of 49.6%, 53.7%, and 72.0%, respectively.
Target
PD-1 & VEGF
Conjugated
Unconjugated
Aplicación
ELISA, FACS, Functional assay
Chemical Information
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SMILES
[JS207]
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
JS207 Related Classifications
HY-P992548 Related Classifications
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