MR-L2
Based on 2 publication(s) in Google Scholar
MR-L2 is a reversible and noncompetitive allosteric activator of long-isoform phosphodiesterase-4 (PDE4), activates representative PDE4 long-isoform variants (PDE4A4, PDE4B1, PDE4C3, PDE4D5). MR-L2 suppresses PGE2-induced MDCK cell cyst formation with an EC50 of 1.2 μM.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.37%
- No. CAS: 2374703-19-0
- Fòrmula: C19H16Cl3FN4O
- Peso molecular:441.71
-
Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MR-L2
More
Actividad biológica
|
PDE4 |
MR-L2 (0.3-10 μM; 1 h) significantly suppresses cAMP elevation and cysts formation in MDCK cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Madin-Darby Canine Kidney (MDCK) cell line
-
Concentration:0.3, 1, 3 and 10 μM
-
Incubation Time:1 hour
-
Result:Suppressed cAMP elevation but not enhanced cAMP excretion in MDCK cells. Suppressed cysts formation in MDCK cells with an EC50 value of 1.2 µM. Suppressed the number of cysts formation in 3D culture for both unstimulated and vasopressin-treated culture condition while showed no effect on cell viability.
Chemical Information
-
No. CAS 2374703-19-0
-
Appearance Solid
-
Peso molecular 441.71
-
Fòrmula C19H16Cl3FN4O
-
Color White to light yellow
-
SMILES
ClC1=C(F)C=C(C2=NN(CC(NCC3=CC(Cl)=CC(Cl)=C3)=O)C(CC)=N2)C=C1
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
-
Journal Impact Factor
-
Most Recent
-
J Clin Invest
GLUT9b- and ABCG2-mediated collecting duct urate transport uncover a vasopressin-independent mechanism of renal water reabsorption. [Abstract]2026 Jun 16:e197021. PMID: 42298327 -
PLoS Pathog
PDE4B deficiency aids macrophage differentiation and contributes to Cryptococcus neoformans brain infection. [Abstract]2026 Mar 10;22(3):e1014040. PMID: 41805786
Solvente y solubilidad
DMSO : 83.33 mg/mL (188.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (274 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2639 mL | 11.3196 mL | 22.6393 mL | 56.5982 mL |
| 5 mM | 0.4528 mL | 2.2639 mL | 4.5279 mL | 11.3196 mL | |
| 10 mM | 0.2264 mL | 1.1320 mL | 2.2639 mL | 5.6598 mL | |
| 15 mM | 0.1509 mL | 0.7546 mL | 1.5093 mL | 3.7732 mL | |
| 20 mM | 0.1132 mL | 0.5660 mL | 1.1320 mL | 2.8299 mL | |
| 25 mM | 0.0906 mL | 0.4528 mL | 0.9056 mL | 2.2639 mL | |
| 30 mM | 0.0755 mL | 0.3773 mL | 0.7546 mL | 1.8866 mL | |
| 40 mM | 0.0566 mL | 0.2830 mL | 0.5660 mL | 1.4150 mL | |
| 50 mM | 0.0453 mL | 0.2264 mL | 0.4528 mL | 1.1320 mL | |
| 60 mM | 0.0377 mL | 0.1887 mL | 0.3773 mL | 0.9433 mL | |
| 80 mM | 0.0283 mL | 0.1415 mL | 0.2830 mL | 0.7075 mL | |
| 100 mM | 0.0226 mL | 0.1132 mL | 0.2264 mL | 0.5660 mL |
MR-L2 Related Classifications
HY-128358 Related Classifications
Powered by Bioz