2. Protein Tyrosine Kinase/RTK Immunology/Inflammation GPCR/G Protein
  3. FLT3 Interleukin Related CCR
  4. Flt-3L-Ig (hum/hum)

Flt-3L-Ig (hum/hum)  (Synonyms: human Flt3L; hFlt3L)

Cat. No.: HY-P991208 Purity: 98%
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Flt-3L-Ig (hum/hum) (hFlt3L) is a Flt3 ligand. Flt-3L-Ig (hum/hum) enhances the release of inflammatory cytokines from myeloid cells and dendritic cells in BRGSF-CBC mice induced by OKT3. Flt-3L-Ig (hum/hum) increases the release of IL-2, CCL2 and CXCL10 in an OKT3-dependent manner. Flt-3L-Ig (hum/hum) can be used in studies related to cytokine release syndrome. Flt-3L-Ig (hum/hum) can be used in studies related to psoriasis-like skin inflammation.

For research use only. We do not sell to patients.

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Flt-3L-Ig (hum/hum) Related Antibodies

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CCR1 CCR2 CCR3 CCR4 CCR5 CCR6 CCR7 CCR8 CCR9

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Description

Flt-3L-Ig (hum/hum) (hFlt3L) is a Flt3 ligand. Flt-3L-Ig (hum/hum) enhances the release of inflammatory cytokines from myeloid cells and dendritic cells in BRGSF-CBC mice induced by OKT3. Flt-3L-Ig (hum/hum) increases the release of IL-2, CCL2 and CXCL10 in an OKT3-dependent manner. Flt-3L-Ig (hum/hum) can be used in studies related to cytokine release syndrome. Flt-3L-Ig (hum/hum) can be used in studies related to psoriasis-like skin inflammation[1][2].

IC50 & Target[1]

IL-2

 

In Vivo

Flt-3L-Ig (hum/hum) (10 μg per injection; i.p.; four times every 2-3 days) boosts human myeloid and dendritic cell compartments in BRGSF-CBC mice, significantly enhancing multiple OKT3-induced CRS features including cytokine release, body weight loss, temperature reduction, immune cell depletion, and immune cell activation[1].
Flt-3L-Ig (hum/hum) (10 μg; i.p.; on day 0, 2, 4, and 7) increases immune cell populations in BRGSF-HIS mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BRGSF mice (female, 21-22 weeks old, humanized with umbilical cord blood CD34+ cells)[1]
Dosage: 10 μg per injection
Administration: i.p.; four times on days 0, 2, 4, and 7 (every 2-3 days)
Result: Increased OKT3-induced serum levels of IL-2, CCL2, and CXCL10.
Enhanced OKT3-induced body weight loss, reaching a 13-15% reduction from baseline at 48h post-OKT3.
Induced a significant temperature drop, a ~4-6% reduction from baseline at 24h post-OKT3, an effect not seen in non-boosted mice.
Amplified OKT3-induced depletion of plasmacytoid dendritic cells (pDCs), conventional dendritic cells (cDCs), and monocytes/macrophages.
Enhanced OKT3-induced activation of pDCs, cDCs, and neutrophils, measured by increased percentages of CD86+, CD80+, and CD86+/CD80+ cells in these populations.
Increased absolute and relative numbers of human monocytes/macrophages, pDCs, and cDCs in bone marrow, spleen, and systemic circulation without inducing significant immune cell activation.\nEnabled detection of Blinatumomab-induced CRS features, including significant increases in serum levels of human TNF-α, IL-6, IFN-γ, CCL2, and CXCL10, and a sustained, significant reduction in body temperature at the 5 μg/kg Blinatumomab dose.\nEnabled detection of JNJ-induced CRS features, including dose-dependent increases in serum levels of human CCL5, CCL2, CXCL8, TNF-α, IL-1RA, and IL-10.
Caused significant depletion of monocytes/macrophages and cDCs at 48h post-JNJ injection.
Induced significant increases in the percentage of CD86+ activated monocytes/macrophages and cDCs at 24h post-JNJ injection (at the 20 mg/kg JNJ dose).
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

86.938 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Flt-3L-Ig (hum/hum)]
Shipping

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
  • Loaded Flt-3L-Ig (hum/hum) on AHC2 biosensor, can bind FLT3 Protein, Human (T227M, HEK293, His, HY-P75183) with an affinity constant of 2.041E-08 M as determined in BLI assay.
Purity & Documentation
References
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Flt-3L-Ig (hum/hum) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Flt-3L-Ig (hum/hum)human Flt3L hFlt3LFLT3Interleukin RelatedCCRCluster of differentiation antigen 135CD135Fms like tyrosine kinase 3ILCC chemokine receptorcytokine release syndromehuman myeloid/dendritic progenitorsconventional dendritic cellshuman monocytes/macrophagesplasmacytoid dendritic cellsBRGSF-HIS miceFlk2psoriasis-like skin inflammationBRGSF micehuman dendritic cellsInhibitorinhibitorinhibit

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