Cat. No.: HY-N0387

Rhynchophylline

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Rhyncholphylline is an alkaloid compound isolated from Uncaria rhynchophyllum. Rhyncholphylline is an EphA4 inhibitor. It has high biological activity and is widely used in anti-inflammatory, neuroprotective and other research..

For research use only. We do not sell to patients.

Purity: 99.75%
CAS No.: 76-66-4
Formula: C₂₂H₂₈N₂O₄
Molecular Weight:384.47

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Rhynchophylline Chemical Structure

CAS No.: 76-66-4

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All NF-κB Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	>40 μM Compound: 4	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 21070010]
Microglia	IC₅₀	18.5 μM Compound: 5	Inhibition of LPS-stimulated NO release in rat cortical microglial cells assessed as nitrite accumulation after 48 hrs Inhibition of LPS-stimulated NO release in rat cortical microglial cells assessed as nitrite accumulation after 48 hrs	[PMID: 18588343]
N2a	IC₅₀	7.9 μM Compound: 1	Inhibition of Kv channel in depolarized mouse N2A cells assessed as inhibition of +30 mV-triggered steady state potassium current flow Inhibition of Kv channel in depolarized mouse N2A cells assessed as inhibition of +30 mV-triggered steady state potassium current flow	[PMID: 19331340]
SW480	IC₅₀	>40 μM Compound: 4	Cytotoxicity against human SW480 cells after 48 hrs by MTT assay Cytotoxicity against human SW480 cells after 48 hrs by MTT assay	[PMID: 21070010]

In Vitro

Rhyncholphylline (0-30 μM, 48 h) inhibits the NO production, release of proinflammatory cytokines (TNF-α and IL-1β) in LPS-activated N9 microglial cells^[1].
Rhyncholphylline (0-30 μM, 48 h) inhibits ERK and p38 MAPK phosphorylation in LPS-treated N9 microglial cells^[1].
Rhyncholphylline inhibits ephrin-A1 induced tyrosine phosphorylation in rat cortical neurons^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

LPS (1 μg/mL) treated N9 cells
Concentration:

0-30 μM
Incubation Time:

48 h
Result:

Inhibited phosphorylated ERK and p38 MAPK level.
Restored IκBα level

In Vivo

Rhynchophylline (10 and 30 mg/kg, i.p., once daily for 4 days) protects rats from pMCAO surgery induced ischemic brain damage^[2].
Rhynchophylline (100 µM, 2 µL, injected bilaterally in the hippocamp) shows neuroprotective effects against Aβ1-42-induced neurotoxicity in rats^[5].
Rhynchophylline (50 mg/kg, p.o., daily, 3-4 weeks) inhibits EphA4 activation in the hippocampus of APP/PS1 mice^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Aβ1-42(1.6 µM, 2 µL) treated rats^[5]
Dosage:

100 µM, 2 µL
Administration:

injected bilaterally in the hippocamp
Result:

Rescued the soluble Aβ1-42-induced spatial learning and memory deficits.
Inhibited Aβ1-42-induced excessive activation of extrasynaptic NMDARs.

Chemical Information

CAS No. 76-66-4
Appearance Solid
Molecular Weight 384.47
Formula C₂₂H₂₈N₂O₄
Color White to off-white
SMILES

O=C(NC1=C2C=CC=C1)[C@]32[C@@](C[C@H](/C(C(OC)=O)=C\OC)[C@@H](CC)C4)([H])N4CC3
Structure Classification
- Alkaloids
- Indole Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

All Cell Proliferation/Viability AssayHistological Imaging/StainingIFELISAWB

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Journal Impact Factor

Mol Cell Biochem

Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signaling. [Abstract]

2019 Nov;461(1-2):205-212. PMID: 31420791

Rhynchophylline purchased from MedChemExpress. Usage Cited in: Mol Cell Biochem. 2019 Nov;461(1-2):205-212. [Abstract]

Administration of Rhy (Rhynchophylline) inhibits MAPK/NF-κB signaling n migraine rats. The administration of Rhy downregulates the expression of p-ERK1/2, p-JNK, p-p38, p-IκBα, nuclear NF-κB p65 while upregulates the expression of IκBα in TNC tissues. Low dose: Rhy of 10 mg/kg. High dose: Rhy of 30 mg/kg.
Hum Exp Toxicol

Rhynchophylline promotes microglia phenotypic transformation and repair of cerebral ischaemic injury through the JAK2/STAT3 pathway. [Abstract]

2025 Jan-Dec:44:9603271251324582. PMID: 40014666

Rhynchophylline purchased from MedChemExpress. Usage Cited in: Hum Exp Toxicol. 2025 Jan-Dec:44:9603271251324582. [Abstract]

The survival rate of microglia in various concentrations of the RIN (0, 2, 4, 8, 10, 20, 40, 100 μM) group.

Rhynchophylline purchased from MedChemExpress. Usage Cited in: Hum Exp Toxicol. 2025 Jan-Dec:44:9603271251324582. [Abstract]

Histopathological changes were observed by HE staining treated with Rhynchophylline (RIN) (30 mg/kg, i.p.).

Rhynchophylline purchased from MedChemExpress. Usage Cited in: Hum Exp Toxicol. 2025 Jan-Dec:44:9603271251324582. [Abstract]

Representative immunofluorescence images of CD86-positive cells in the cerebral cortex tissue of rats treated with Rhynchophylline (RIN) (30 mg/kg, i.p.).

Rhynchophylline purchased from MedChemExpress. Usage Cited in: Hum Exp Toxicol. 2025 Jan-Dec:44:9603271251324582. [Abstract]

Detection of M1 and M2 cytokines utilizing ELISA techniques treated with Rhynchophylline (RIN) (30 mg/kg, i.p.).

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (65.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6010 mL

13.0049 mL

26.0098 mL

5 mM

0.5202 mL

2.6010 mL

5.2020 mL

10 mM

0.2601 mL

1.3005 mL

2.6010 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

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Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.50 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Dan Yuan, et al. Anti-inflammatory effects of rhynchophylline and isorhynchophylline in mouse N9 microglial cells and the molecular mechanism. International Immunopharmacology Volume 9, Issues 13–14, December 2009, Pages 1549–1554

[2]. Houcai Huang, et al. Neuroprotective Effects of Rhynchophylline Against Ischemic Brain Injury via Regulation of the Akt/mTOR and TLRs Signaling Pathways. Molecules 2014, 19 (8): 11196-11210; doi:10.3390/molecules190811196

[3]. Tai-Hyun Kang, et al. Protective effect of rhynchophylline and isorhynchophylline on in vitro ischemia-induced neuronal damage in the hippocampus: putative neurotransmitter receptors involved in their action. Life Sciences Volume 76, Issue 3, 3 December 2004, Pages 331–343

[4]. Kinzo Matsumoto, et al. Suppressive effects of isorhynchophylline on 5-HT2A receptor function in the brain: Behavioural and electrophysiological studies. European Journal of Pharmacology Volume 517, Issue 3, 11 July 2005, Pages 191–199

[5]. Yang Y, et al. Rhynchophylline suppresses soluble Aβ1-42-induced impairment of spatial cognition function via inhibiting excessive activation of extrasynaptic NR2B-containing NMDA receptors. Neuropharmacology. 2018 Jun;135:100-112. [Content Brief]

[6]. Fu AK, et al. Blockade of EphA4 signaling ameliorates hippocampal synaptic dysfunctions in mouse models of Alzheimer's disease. Proc Natl Acad Sci U S A. 2014 Jul 8;111(27):9959-64. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6010 mL	13.0049 mL	26.0098 mL	65.0246 mL
	5 mM	0.5202 mL	2.6010 mL	5.2020 mL	13.0049 mL
	10 mM	0.2601 mL	1.3005 mL	2.6010 mL	6.5025 mL
	15 mM	0.1734 mL	0.8670 mL	1.7340 mL	4.3350 mL
	20 mM	0.1300 mL	0.6502 mL	1.3005 mL	3.2512 mL
	25 mM	0.1040 mL	0.5202 mL	1.0404 mL	2.6010 mL
	30 mM	0.0867 mL	0.4335 mL	0.8670 mL	2.1675 mL
	40 mM	0.0650 mL	0.3251 mL	0.6502 mL	1.6256 mL
	50 mM	0.0520 mL	0.2601 mL	0.5202 mL	1.3005 mL
	60 mM	0.0433 mL	0.2167 mL	0.4335 mL	1.0837 mL

Keywords

Rhynchophylline

2 Cited Publications

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Rhynchophylline

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Publications Citing Use of MedChemExpress (MCE) Rhynchophylline

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Rhynchophylline Related Classifications

HY-N0387 Related Classifications