Cat. No.: HY-N3005

Britannin

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Britannin is an NLRP3 inhibitor with an IC₅₀ of 3.630 μM, exhibiting anti-inflammatory activity. Britannin inhibits the activation and assembly of the NLRP3 inflammasome by blocking the interaction between NLRP3 and NEK7. Additionally, Britannin demonstrates antitumor activity by inhibiting the proliferation of tumor cells through blocking the interaction between HIF-1α and Myc, thereby suppressing PD-L1 expression and enhancing cytotoxic T lymphocyte activity. Britannin can also induce apoptosis and autophagy in liver cancer cells by activating ROS-regulated AMPK. Britannin holds promise for research in the fields of anti-inflammatory and antitumor therapeutics.

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Pureté: 99.93%
CAS No.: 33627-28-0
Formule: C₁₉H₂₆O₇
Masse moléculaire:366.41

Stockage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Britannin Chemical Structure

CAS No.: 33627-28-0

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

NLRP3

3.630 μM (IC₅₀)

Voir tous les produits spécifiques à Isoform NOD-like Receptor (NLR) :

Cellular Effect

Cell Line	Type	Value	Description	References
RAW264.7	IC₅₀	0.11 μM Compound: 6	Inhibition of LPS-induced NO production in mouse RAW264.7 cells measured after 24 hrs Inhibition of LPS-induced NO production in mouse RAW264.7 cells measured after 24 hrs	[PMID: 20515062]

In Vitro

Britannin (1, 5, 10 μM, 30 min) inhibits NLRP3-mediated pyroptosis in mouse macrophages^[4].
Britannin (1, 5, 10 μM, 30 min) inhibits activation of the NLRP3 inflammasome in an NF-κB-independent manner^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:

Bone marrow-derived macrophages(BMDMs)
Concentration:

1, 5, 10 μM
Incubation Time:

30 min
Result:

Dose-dependently inhibited secretion of the cleaved Caspase-1 and the mature IL-1β, and suppressed NLRP3-mediated pyroptosis in murine macrophages.

In Vivo

Britannin (20 mg/kg, i.p., once daily for 3 consecutive days) ameliorates lung injury and systemic infection in Lipopolysaccharides (LPS) (HY-D1056) -induced acute lung injury mouse model in an NLRP3-dependent manner^[4].
Britannin (20 mg/kg, i.p., once every other day, for a total of 3 injections) relieves gouty arthritis in Monosodium urate (HY-B2130A) -induced gouty arthritis mouse model by inhibiting NLRP3 activation^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Monosodium urate (HY-B2130A) -induced gouty arthritis mouse model, Lipopolysaccharides (LPS) (HY-D1056) -induced acute lung injury mouse model^[4]
Dosage:

20 mg/kg
Administration:

Intraperitoneal injection (i.p.), inject for three consecutive days (acute lung injury mouse model) or once every other day, for a total of 3 injections (Monosodium urate (HY-B2130A) -induced gouty arthritis mouse model)
Result:

Significantly suppressed the changes in footpad joint size, alleviated the migration of inflammatory cells in Monosodium urate (HY-B2130A) -induced gouty arthritis mouse model and nhibited the secretion of IL-1β and the activation of Caspase-1, demonstrating that Britannin prevented gouty arthritis through anti-inflammatory effects.
Suppressed pulmonary edema, breakdown of the pneumatic blood barrier, and cell infiltration in lung tissues and pathological injury and inflammatory cell infiltration in lung tissues, were ameliorated by Britannin treatment.

Chemical Information

CAS No. 33627-28-0
Appearance Solid
Masse moléculaire 366.41
Formule C₁₉H₂₆O₇
Color White to off-white
SMILES

C[C@]12[C@@]([C@@H](C[C@@](O3)([H])[C@](C(C3=O)=C)([H])[C@@H]2OC(C)=O)C)([H])[C@@H](OC(C)=O)C[C@@H]1O
Structure Classification
- Terpenoids
- Sesquiterpenes
Initial Source
- Plants
- Compositae
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Exp Ther Med

Britannin inhibits cell proliferation, migration and glycolysis by downregulating KLF5 in lung cancer. [Abstract]

2024 Jan 22;27(3):109. PMID: 38361511

Solvant et solubilité

In Vitro:

DMSO : 250 mg/mL (682.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7292 mL

13.6459 mL

27.2918 mL

5 mM

0.5458 mL

2.7292 mL

5.4584 mL

10 mM

0.2729 mL

1.3646 mL

2.7292 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (284 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
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MS (2659 KB)
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IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Cui YQ The suppressive effects of Britannin (Bri) on human liver cancer through inducing apoptosis and autophagy via AMPK activation regulated by ROS. Biochem Biophys Res Commun. 2018 Mar 11;497(3):916-923. [Content Brief]

[2]. Park HH, et al. Britanin suppresses LPS-induced nitric oxide, PGE2 and cytokine production via NF-κB and MAPK inactivation in RAW 264.7 cells. Int Immunopharmacol. 2013 Feb;15(2):296-302. [Content Brief]

[3]. Moghadam MH, et al. Anti-proliferative activity and apoptotic potential of britannin, a sesquiterpene lactone from Inula aucheriana. Nat Prod Commun. 2012 Aug;7(8):979-80. [Content Brief]

[4]. Shao, Jj., Li, Wf., Sun, Jf. et al. Britannin as a novel NLRP3 inhibitor, suppresses inflammasome activation in macrophages and alleviates NLRP3-related diseases in mice. Acta Pharmacol Sin, 2024, 45, 803–814. [Content Brief]

[5]. Zhang YF, et al. Britannin stabilizes T cell activity and inhibits proliferation and angiogenesis by targeting PD-L1 via abrogation of the crosstalk between Myc and HIF-1α in cancer. Phytomedicine. 2021 Jan;81:153425. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7292 mL	13.6459 mL	27.2918 mL	68.2296 mL
	5 mM	0.5458 mL	2.7292 mL	5.4584 mL	13.6459 mL
	10 mM	0.2729 mL	1.3646 mL	2.7292 mL	6.8230 mL
	15 mM	0.1819 mL	0.9097 mL	1.8195 mL	4.5486 mL
	20 mM	0.1365 mL	0.6823 mL	1.3646 mL	3.4115 mL
	25 mM	0.1092 mL	0.5458 mL	1.0917 mL	2.7292 mL
	30 mM	0.0910 mL	0.4549 mL	0.9097 mL	2.2743 mL
	40 mM	0.0682 mL	0.3411 mL	0.6823 mL	1.7057 mL
	50 mM	0.0546 mL	0.2729 mL	0.5458 mL	1.3646 mL
	60 mM	0.0455 mL	0.2274 mL	0.4549 mL	1.1372 mL
	80 mM	0.0341 mL	0.1706 mL	0.3411 mL	0.8529 mL
	100 mM	0.0273 mL	0.1365 mL	0.2729 mL	0.6823 mL

Keywords

Britannin

1 Cited Publications

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Britannin

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Publications Citing Use of MedChemExpress (MCE) Britannin

Voir tous les produits spécifiques à Isoform NOD-like Receptor (NLR)

Activité biologique

Chemical Information

Publications (1)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Britannin Related Classifications

HY-N3005 Related Classifications