Dantrolene
Based on 17 publication(s) in Google Scholar
Dantrolene is an orally active, non-competitive glutathione reductase inhibitor with a Ki of 111.6 μM and an IC50 of 52.3 μM. Dantrolene is also a calcium channel protein inhibitor. Dantrolene inhibits the release of Ca2+ from RyR1 and RyR3, which can be beneficial in a variety of pathologies caused by disruptions in calcium homeostasis (e.g., stroke, ischemia/reperfusion injury, and neurodegenerative diseases). Dantrolene offers relief of muscle spasms, malignant hyperthermia, and antitoxic, antipyretic, and anti-inflammatory properties.
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- Pureté: 99.93%
- CAS No.: 7261-97-4
- Formule: C14H10N4O5
- Masse moléculaire:314.26
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Dantrolene
More- Cell Res. 2022 Mar;32(3):288-301. [Abstract]
- Nat Commun. 2025 Nov 18;16(1):10110. [Abstract]
- Anim Nutr. 2021 Dec;7(4):1115-1123. [Abstract]
- J Anim Sci Biotechnol. 2022 Feb 11;13(1):9. [Abstract]
- Cell Rep. 2025 Apr 16;44(5):115572. [Abstract]
- J Med Chem. 2025 Apr 17. [Abstract]
- Front Immunol. 2021 Jul 7:12:688674. [Abstract]
- Biochem Pharmacol. 2025 Oct:240:117120. [Abstract]
- Int J Mol Sci. 2026 May 14;27(10):4373. [Abstract]
- Thromb Haemost. 2025 Mar 6. [Abstract]
- ACS Omega. 2020 Oct 12;5(41):26551-26561. [Abstract]
- J Cell Commun Signal. 2023 Dec;17(4):1309-1321. [Abstract]
- Physiol Rep. 2026 Jun;14(11):e70975. [Abstract]
- Biocell. 2026 Jan 23.
- bioRxiv. 2024 Dec 23:2024.12.23.629519. [Abstract]
- Res Sq. 2024 Jun 11.
- Research Square Preprint. 2021 May.
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Activité biologique
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Cell Line
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Type | Value | Description | References |
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| HEK293 | EC50 |
0.059 μM
Compound: Dantrolene
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Inhibition of RyR1 harboring R2163C mutant (unknown origin) stably expressed in HEK293 cells coexpressing R-CEPIA1er assessed as suppression in Ca2+ leakage from ER prestimulated with doxycycline for 24 to 28 hrs followed by compound addition at 100 secs
Inhibition of RyR1 harboring R2163C mutant (unknown origin) stably expressed in HEK293 cells coexpressing R-CEPIA1er assessed as suppression in Ca2+ leakage from ER prestimulated with doxycycline for 24 to 28 hrs followed by compound addition at 100 secs
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[PMID: 31299492] |
Dantrolene (50 μM) sodium alleviates malignant hyperthermia by preventing overactive Ca2+ release by increasing the affinity of ryanodine receptor (RyR) for Mg2+ in rat skin fibers[2].
Dantrolene (30 μM, 1 h-3 days) sodium ameliorates the impairment of neurogenesis and synaptogenesis, in association with restoring intracellular Ca2+ homeostasis and physiological autophagy, cell survival and proliferation in induced pluripotent stem cells derived from patients with Alzheimer's disease [3].
Dantrolene (40 μM, 12 h) sodium enhances the protective effect of hypothermia on cerebral cortex neurons, including improved morphology, increased survival and mitochondrial membrane potential, decreased intracellular reactive oxygen species, cytoplasmic histone-associated DNA fragmentation and apoptosis[7].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Induced pluripotent stem cells from patients with familiar and sporadic Alzheimer’s disease
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Concentration:30 μM
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Incubation Time:3 days
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Result:Promoted differentiation of neuroprogenitor cell derived from sporadic Alzheimer’s disease/familial Alzheimer’s disease induced pluripotent stem cells into immature neurons at differentiation day 23.
Promoted the percentage of Trb1 positive cells.
Promoted differentiation into basal forebrain cholinergic neurons.
Dantrolene (5-10 mg/kg, i.p.) sodium attenuates spinal cord inflammation in mice with experimental autoimmune encephalomyelitis (EAE) [5].
Dantrolene (5 mg/kg, intranasal or subcutaneous, 3 times per week) sodium improves cognitive dysfunction in 5XFAD Alzheimer's disease (AD) mice [6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 male mice (EAE was induced by subcutaneous administration of a total of 300 μg in the flanks (each flank received 150 μg) of rodent myelin oligodendrocyte glycoprotein peptide 35–55 (MEVGWYRSPFSRVVHLYRNGK, New England Peptide) emulsified in the complete Freund’s adjuvant containing Mycobacterium tuberculosis H37Ra[5]
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Dosage:5, 10 mg/kg
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Administration:Intraperitoneal injection (i.p.)
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Result:Reduced the severity of EAE clinical symptoms and dampened inflammation in the spinal cord
Caused skeletal muscle weakness at 5 mg/kg.
Did not display noticeable upright movement deficits on flat-surface at 5 mg/kg.
Chemical Information
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CAS No. 7261-97-4
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Appearance Solid
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Masse moléculaire 314.26
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Formule C14H10N4O5
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Color Light yellow to yellow
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SMILES
O=C1NC(CN1/N=C/C2=CC=C(C3=CC=C([N+]([O-])=O)C=C3)O2)=O
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Synonyms
F 368
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (17)
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Journal Impact Factor
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Most Recent
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Cell Res
Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate. [Abstract]2022 Mar;32(3):288-301. PMID: 35121857 -
Nat Commun
Crystal structures of Ryanodine Receptor reveal dantrolene and azumolene interactions guiding inhibitor development. [Abstract]2025 Nov 18;16(1):10110. PMID: 41253812
Dantrolene purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10110. [Abstract]
Dose-dependent effects of Dantrolene (DAN) and AZU on ER luminal [Ca2+] changes in cell lines expressing RyR1 R2163C. The reslts showed that effectively Dantrolene (DAN) inhibited Ca2+ leakage, with IC50 value of 0.26 μM.
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Anim Nutr
Arginine promotes myogenic differentiation and myotube formation through the elevation of cytoplasmic calcium concentration. [Abstract]2021 Dec;7(4):1115-1123. PMID: 34738042 -
J Anim Sci Biotechnol
Ryanodine receptor RyR1-mediated elevation of Ca2+ concentration is required for the late stage of myogenic differentiation and fusion. [Abstract]2022 Feb 11;13(1):9. PMID: 35144690 -
Cell Rep
2025 Apr 16;44(5):115572. PMID: 40249703 -
J Med Chem
A Novel Compound 3a-M1, from Metabolites of Sinomenine Derivative 3a, Exerts Potent Anti-Aplastic Anemia Activity via IP3R/ORAI-Mediated CTL Ferroptosis. [Abstract]2025 Apr 17. PMID: 40243551 -
Front Immunol
Inflammasome-Induced Osmotic Pressure and the Mechanical Mechanisms Underlying Astrocytic Swelling and Membrane Blebbing in Pyroptosis. [Abstract]2021 Jul 7:12:688674. PMID: 34305921 -
Biochem Pharmacol
Dual inhibition of EGR1/STAT3 transcriptional hubs suppresses macrophage-driven liver fibrosis: A multi-omics-guided drug repurposing strategy. [Abstract]2025 Oct:240:117120. PMID: 40623460 -
Int J Mol Sci
Bufalin Suppresses Pancreatic Ductal Adenocarcinoma Through ER Stress-Ferroptosis Crosstalk Associated with IP3R-Linked Ca2+ Dysregulation and ATF3/SLC7A11 Regulation. [Abstract]2026 May 14;27(10):4373. PMID: 42196362 -
Thromb Haemost
Inhibition of IP3 (Inositol 1,4,5-Trisphosphate) Receptors Retards SARS-CoV-2-Induced Endothelial von Willebrand Factor Secretion and Thrombosis. [Abstract]2025 Mar 6. PMID: 40049586 -
ACS Omega
2020 Oct 12;5(41):26551-26561. PMID: 33110983 -
J Cell Commun Signal
Insights into the mediation of Ca2+ signaling in the promoting effects of LETX-VI on the synthesis and release of dopamine. [Abstract]2023 Dec;17(4):1309-1321. PMID: 37702818
Dantrolene purchased from MedChemExpress. Usage Cited in: J Cell Commun Signal. 2023 Dec;17(4):1309-1321. [Abstract]
Effect of 2 µM LETX-VI treatment on cytosolic Ca2+ level when RyRs in ER membrane was inhibited by Dantrolene (30 µM). The results demonstrated that blockade of RyR by Dantrolene decreased the F340/F380 ratio, indicating that RyR is involved in LETX-VI-induced Ca2+ release from the endoplasmic reticulum.
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Physiol Rep
Monosodium glutamate-mediated Ca2+-dependent intestinal epithelial ion transports in health and IBS-D in male mice. [Abstract]2026 Jun;14(11):e70975. PMID: 42281391 -
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bioRxiv
Angiotensin II elicits robust calcium oscillations coordinated within juxtaglomerular cell clusters to suppress renin secretion. [Abstract]2024 Dec 23:2024.12.23.629519. PMID: 39763801 -
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Solvant et solubilité
DMSO : 16.67 mg/mL (53.05 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
The following protocol is derived from the literature and is for reference only. It is recommended to first try a small sample.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Pureté et documentation
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Fiche technique (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Zhang W, et al. Neuroprotective effects of dantrolene in neurodegenerative disease: Role of inhibition of pathological inflammation. J Anesth Transl Med. 2024 Jun;3(2):27-35. [Content Brief]
[2]. Choi RH, et al. Dantrolene requires Mg2+ to arrest malignant hyperthermia. Proc Natl Acad Sci U S A. 2017 May 2;114(18):4811-4815. [Content Brief]
[3]. Wang Y, et al. Dantrolene Ameliorates Impaired Neurogenesis and Synaptogenesis in Induced Pluripotent Stem Cell Lines Derived from Patients with Alzheimer's Disease. Anesthesiology. 2020 May;132(5):1062-1079. [Content Brief]
[4]. Zardast M, et al. Evaluation of the Effectiveness of Dantrolene against Digoxininduced Cardiotoxicity in Adult Rats. Cardiovasc Hematol Agents Med Chem. 2023;22(1):60-65. [Content Brief]
[5]. Osipchuk NC, et al. Modulation of Ryanodine Receptors Activity Alters the Course of Experimental Autoimmune Encephalomyelitis in Mice. Front Physiol. 2021 Dec 17;12:770820. [Content Brief]
[6]. Shi Y, et al. Intranasal Dantrolene as a Disease-Modifying Drug in Alzheimer 5XFAD Mice. J Alzheimers Dis. 2020;76(4):1375-1389. [Content Brief]
[7]. Xu SY, et al. Dantrolene enhances the protective effect of hypothermia on cerebral cortex neurons. Neural Regen Res. 2015 Aug;10(8):1279-85. [Content Brief]
[8]. F Zhao, et al. Dantrolene inhibition of ryanodine receptor Ca2+ release channels. Molecular mechanism and isoform selectivity. J Biol Chem. 2001 Apr 27;276(17):13810-6. [Content Brief]
[9]. Christopher B Sylvester, et al. Dantrolene inhibits lysophosphatidylcholine-induced valve interstitial cell calcific nodule formation via blockade of the ryanodine receptor. Front Cardiovasc Med. 2023 Mar 30:10:1112965. [Content Brief]
[10]. Xi Chen, et al. Dantrolene is neuroprotective in Huntington's disease transgenic mouse model. Mol Neurodegener. 2011 Nov 25;6:81. [Content Brief]
[11]. Büyükokuroğlu ME. Anti-inflammatory and antinociceptive properties of Dantrolene in rats and mice. Pharmacol Res. 2002 Jun;45(6):455-60. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.1821 mL | 15.9104 mL | 31.8208 mL | 79.5520 mL |
| 5 mM | 0.6364 mL | 3.1821 mL | 6.3642 mL | 15.9104 mL | |
| 10 mM | 0.3182 mL | 1.5910 mL | 3.1821 mL | 7.9552 mL | |
| 15 mM | 0.2121 mL | 1.0607 mL | 2.1214 mL | 5.3035 mL | |
| 20 mM | 0.1591 mL | 0.7955 mL | 1.5910 mL | 3.9776 mL | |
| 25 mM | 0.1273 mL | 0.6364 mL | 1.2728 mL | 3.1821 mL | |
| 30 mM | 0.1061 mL | 0.5303 mL | 1.0607 mL | 2.6517 mL | |
| 40 mM | 0.0796 mL | 0.3978 mL | 0.7955 mL | 1.9888 mL | |
| 50 mM | 0.0636 mL | 0.3182 mL | 0.6364 mL | 1.5910 mL |
Dantrolene Related Classifications
HY-12542 Related Classifications
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