2-Fluoroadenine
Based on 1 Customer Validation
2-Fluoroadenine is a toxic purine bases. 2-Fluoroadenine has toxicity in nonproliferating and proliferating tumor cells. 2-Fluoroadenine can be used for researching anticancer.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.68%
- No. CAS: 700-49-2
- Fòrmula: C5H4FN5
- Peso molecular:153.12
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Ver todos los productos específicos de isoformas DNA/RNA Synthesis
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Actividad biológica
DNA/RNA synthesis[1]
2-Fluoroadenine (2 μM; 4 hours) inhibits CEM cell growth by targeting one or more enzymes involved in either RNA or protein synthesis[1].
2-Fluoroadenine (0-1000 μM; 96 hours) exhibits cytotoxicity in nonproliferating MRC-5 cells[1].
2-Fluoroadenine (0.22, 2.2 and 22 μM; 30 hours) inhibits protein, RNA, and DNA syntheses in Balb-3T3 cells incubated in serum-free medium[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:CEM cells[1]
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Concentration:2 μM
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Incubation Time:4 hours
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Result:Inhibited CEM cell growth by targeting one or more enzymes involved in either RNA or protein synthesis.
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Cell Line:MRC-5 cells[1]
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Concentration:0-1000 μM
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Incubation Time:4 hours
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Result:Exhibited cytotoxicity in nonproliferating MRC-5 cells.
Chemical Information
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No. CAS 700-49-2
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Appearance Solid
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Peso molecular 153.12
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Fòrmula C5H4FN5
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Color White to yellow
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SMILES
NC1=NC(F)=NC2=C1NC=N2
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvente y solubilidad
DMSO : 20 mg/mL (130.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (13.06 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.5308 mL | 32.6541 mL | 65.3083 mL | 163.2706 mL |
| 5 mM | 1.3062 mL | 6.5308 mL | 13.0617 mL | 32.6541 mL | |
| 10 mM | 0.6531 mL | 3.2654 mL | 6.5308 mL | 16.3271 mL | |
| 15 mM | 0.4354 mL | 2.1769 mL | 4.3539 mL | 10.8847 mL | |
| 20 mM | 0.3265 mL | 1.6327 mL | 3.2654 mL | 8.1635 mL | |
| 25 mM | 0.2612 mL | 1.3062 mL | 2.6123 mL | 6.5308 mL | |
| 30 mM | 0.2177 mL | 1.0885 mL | 2.1769 mL | 5.4424 mL | |
| 40 mM | 0.1633 mL | 0.8164 mL | 1.6327 mL | 4.0818 mL | |
| 50 mM | 0.1306 mL | 0.6531 mL | 1.3062 mL | 3.2654 mL | |
| 60 mM | 0.1088 mL | 0.5442 mL | 1.0885 mL | 2.7212 mL | |
| 80 mM | 0.0816 mL | 0.4082 mL | 0.8164 mL | 2.0409 mL | |
| 100 mM | 0.0653 mL | 0.3265 mL | 0.6531 mL | 1.6327 mL |
2-Fluoroadenine Related Classifications
HY-W008469 Related Classifications
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