CID-1067700
Based on 15 publication(s) in Google Scholar
CID-1067700 (ML282) is a pan GTPase inhibitor, and competitively inhibits Ras-related in brain 7 (Rab7) with a Ki of 13 nM.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.71%
- No. CAS: 314042-01-8
- Fòrmula: C18H18N2O4S2
- Peso molecular:390.48
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Publications Citing Use of MedChemExpress (MCE) CID-1067700
More- Nat Mater. 2025 Nov 5. [Abstract]
- Immunity. 2025 Jul 16:S1074-7613(25)00287-0. [Abstract]
- Autophagy. 2024 Dec 11:1-18. [Abstract]
- Adv Sci (Weinh). 2024 Jun 5:e2401676. [Abstract]
- Phytomedicine. 2024 Jan:122:155079. [Abstract]
- Alzheimers Res Ther. 2025 Jun 9;17(1):132. [Abstract]
- Int J Biol Macromol. 2024 Sep 17:135764. [Abstract]
- Nutrients. 2022 Feb 4;14(3):658. [Abstract]
- J Biol Chem. 2024 Jun;300(6):107348. [Abstract]
- J Virol. 2023 Dec 21;97(12):e0133823. [Abstract]
- Toxins. 2026 Apr 15;18(4):186. [Abstract]
- Mol Cell Biol. 2025;45(3):99-115. [Abstract]
- Vet Microbiol. 2023 Sep:284:109794. [Abstract]
- Res Sq. 2023 Sep 22:rs.3.rs-3372987. [Abstract]
- Research Square Preprint. 2021 Sep.
Customer Validation & Images
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In Vivo Efficacy Study
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Actividad biológica
Ki: 13 nM (Rab7)[1]
CID-1067700 (ML282) is a pan GTPase inhibitor, and competitively inhibits Rab7 with a Ki of 13 nM. CID-1067700 shows inhibitory activity against nucleotide binding by Rab7, with Kds of 100 nM and 40 nM for BODIPY-GTP and BODIPY-GDP, respectively. With increasing concentration, CID-1067700 causes strong inhibition on binding of the BODIPY-linked nucleotides, with EC50 values of 11.22 ± 1.34 nM for BODIPY-GTP and 20.96 ± 1.34 nM for BODIPY-GDP and calculated Ki values of 12.89 nM and 19.70 nM respectively. CID-1067700 (10 μM) has no effect on the rate of release of bound BODIPY-linked nucleotide by wild type Rab7 under equilibrium binding conditions[1]. CID-1067700 (0-40 μM) inhibits Rab7 activity, NF-κB activation as well as AID induction in B cells. Furthermore, CID-1067700 binds Rab7 with a high affinity (EC50: 10-20 nM), and blocks Class switch DNA recombination (CSR) in B cells via targeting Rab7[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 314042-01-8
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Appearance Solid
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Peso molecular 390.48
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Fòrmula C18H18N2O4S2
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Color Light yellow to yellow
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SMILES
O=C(C1=C(NC(NC(C2=CC=CC=C2)=O)=S)SC3=C1CC(C)(C)OC3)O
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Synonyms
ML282
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (15)
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Journal Impact Factor
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Most Recent
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Nat Mater
2025 Nov 5. PMID: 41193876 -
Immunity
Gasdermin C cleavage by Cathepsin S modulates Rab7 vesicles in intestinal epithelial cells to amplify anti-helminth immunity. [Abstract]2025 Jul 16:S1074-7613(25)00287-0. PMID: 40701157 -
Autophagy
USP4 depletion-driven RAB7A ubiquitylation impairs autophagosome-lysosome fusion and aggravates periodontitis. [Abstract]2024 Dec 11:1-18. PMID: 39663592
CID-1067700 purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Dec 11:1-18. [Abstract]
Western blot analysis of SQSTM1, LC3, total RAB7A, and GTP-RAB7A (immunoprecipitated by anti-GTP-RAB7A antibody) in four groups as indicated in THP-1 cells. Cells were treated with CID-1067700 (100 μM), ML098 (100 nM), or Baf A1 (50 nM) for 8 hours.
CID-1067700 purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Dec 11:1-18. [Abstract]
GFP-RFP-LC3 THP-1 cells were treated with P. g. (MOI = 100), ML098 (100 nM), and CID-1067700 (100 μM) for 8 hours, and the colocalization of GFP-LC3 and RFP-LC3 was captured to detect autophagy. Red dots represent autolysosomes and yellow dots autophagosomes. Scale bars: 10 μm.
CID-1067700 purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Dec 11:1-18. [Abstract]
Representative micro-CT analysis of alveolar bone in normal control mice and periodontitis mice treated with ML098 (1 mg/kg, intraperitoneally injected every 2 days for 10 days), CID-1067700 (10 mg/kg, intraperitoneally injected every 2 days for 10 days), or USP4 overexpression adenovirus. Scale bars: 500 μm.
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Adv Sci (Weinh)
Targeting Rab7-Rilp Mediated Microlipophagy Alleviates Lipid Toxicity in Diabetic Cardiomyopathy. [Abstract]2024 Jun 5:e2401676. PMID: 38837607 -
Phytomedicine
Timosaponin AIII induces lipid peroxidation and ferroptosis by enhancing Rab7-mediated lipophagy in colorectal cancer cells. [Abstract]2024 Jan:122:155079. PMID: 37863004 -
Alzheimers Res Ther
AP2A1 activates Rab7 to promote axonal autophagosome transport and slow the progression of Alzheimer's disease. [Abstract]2025 Jun 9;17(1):132. PMID: 40490761
CID-1067700 purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Jun 9;17(1):132. [Abstract]
CID1067700 (40 µM, 24 h) markedly suppressed Rab7 activity induced by the overexpression of Flag-AP2A1 in APP cells.
CID-1067700 purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Jun 9;17(1):132. [Abstract]
CID-1067700 (40 µM, 24 h) abrogated the increase in colocalization of autophagosomes (marked by LC3) with DIC1/2 induced by overexpression of Flag-AP2A1 in APP cells.
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Int J Biol Macromol
2024 Sep 17:135764. PMID: 39299429 -
Nutrients
Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis. [Abstract]2022 Feb 4;14(3):658. PMID: 35277017 -
J Biol Chem
Calcium and IL-6 regulate the anterograde trafficking and plasma membrane residence of the iron exporter ferroportin to modulate iron efflux. [Abstract]2024 Jun;300(6):107348. PMID: 38718866 -
J Virol
2023 Dec 21;97(12):e0133823. PMID: 38009916 -
Toxins
Interference of Large Clostridial Glucosyltransferases with the Endolysosomal Pathway: Toxin-Induced Imbalance of Early Endosomes, Functional Lysosomes and Autophagosomes. [Abstract]2026 Apr 15;18(4):186. PMID: 42043050 -
Mol Cell Biol
Kinase Inhibitor-Induced Cell-Type Specific Vacuole Formation in the Absence of Canonical ATG5-Dependent Autophagy Initiation Pathway. [Abstract]2025;45(3):99-115. PMID: 39895059 -
Vet Microbiol
Emodin and rhapontigenin inhibit the replication of African swine fever virus by interfering with virus entry. [Abstract]2023 Sep:284:109794. PMID: 37295229 -
Res Sq
2023 Sep 22:rs.3.rs-3372987. PMID: 37790337 -
Solvente y solubilidad
DMSO : 12.5 mg/mL (32.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 1 mg/mL (2.56 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
To treat human and mouse B cells in vitro with the Rab7 inhibitor, CID-1067700 is diluted in DMSO and added to cell cultures to the final concentration of 40 μM. CID-1067700 or DMSO is added either at the time when B cell stimulation started, or 66 h after B cells are stimulated with LPS plus IL-4, TGF-β, anti-δ/dex and RA, for analysis of plasma cell survival[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[2]
For in vivo treatment, CID-1067700 dissolved in DMSO (stock concentration 40 mM, 16 mg/mL) is diluted with the solvent to the final volume of 50 μL and injected intraperitoneally (i.p.) once per week at the dose of 16 mg/kg body weight. C57, MRL/Faslpr/lpr and C57/Sle1Sle2Sle3 mice injected i. p. with the vehicle DMSO (50 μL). For survival studies and skin lesion analyses, MRL/Faslpr/lpr mice are treated with nil or CID-1067700 for 10 weeks and maintained until moribund (e.g., showing signs of severe loss of mobility, hunched back, piloerection, ruffled fur, dyspnea, gasping and weight loss), at which point they are euthanized[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (289 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Agola JO, et al. A competitive nucleotide binding inhibitor: in vitro characterization of Rab7 GTPase inhibition. ACS Chem Biol. 2012 Jun 15;7(6):1095-108. [Content Brief]
[2]. Lam T, et al. Small Molecule Inhibition of Rab7 Impairs B Cell Class Switching and Plasma Cell Survival To Dampen the Autoantibody Response in Murine Lupus. J Immunol. 2016 Nov 15;197(10):3792-3805. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 2.5610 mL | 12.8048 mL | 25.6095 mL | 64.0238 mL |
| DMSO | 5 mM | 0.5122 mL | 2.5610 mL | 5.1219 mL | 12.8048 mL |
| 10 mM | 0.2561 mL | 1.2805 mL | 2.5610 mL | 6.4024 mL | |
| 15 mM | 0.1707 mL | 0.8537 mL | 1.7073 mL | 4.2683 mL | |
| 20 mM | 0.1280 mL | 0.6402 mL | 1.2805 mL | 3.2012 mL | |
| 25 mM | 0.1024 mL | 0.5122 mL | 1.0244 mL | 2.5610 mL | |
| 30 mM | 0.0854 mL | 0.4268 mL | 0.8537 mL | 2.1341 mL |
CID-1067700 Related Classifications
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