Referencia número: HY-50737

DUB-IN-3

7 Cited Publications

Revisión del cliente

Based on 7 publication(s) in Google Scholar

DUB-IN-3 (compound 22c) is a potent and selective inhibitor of the deubiquitinating enzyme USP8 with an IC₅₀ value of 0.56 μM. DUB-IN-3 is promising for research of cancer, neurodegenerative diseases, inflammation, viral infection and cardiovascular disease.

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Pureza: 99.00%
No. CAS: 924296-17-3
Fòrmula: C₁₆H₉N₅O
Peso molecular:287.28

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

DUB-IN-3 Chemical Structure

No. CAS: 924296-17-3

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (7)
Solvente y solubilidad
- In Vitro
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

IC₅₀ & Target

USP7

>100 μM (IC₅₀)

Ver todos los productos específicos de isoformas Deubiquitinase :

In Vitro

DUB-IN-3 (10 nM-100 μM) affects the viability of HCT116 colon and PC-3 prostate cancer cell lines with IC₅₀ values of 0.5-1.5 μM range^[1].

Inhibitory effect of DUB-IN-3 on USP7 and USP8 deubiquitinating activity (IC₅₀: μM)^[1]

Compd	USP7	USP8
DUB-IN-3	>100	0.56

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

No. CAS 924296-17-3
Appearance Solid
Peso molecular 287.28
Fòrmula C₁₆H₉N₅O
Color Light yellow to yellow
SMILES

C=CCO/N=C1C2=C(N=C(C#N)C(C#N)=N2)C3=CC=CC=C/13
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (7)

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Cell Res

Targeted destruction of VISTA boosts anti-tumor immunotherapy. [Abstract]

2025 Dec;35(12):987-1002. PMID: 41225154
Cell Death Dis

Usp8 promotes tumor cell migration through activating the JNK pathway. [Abstract]

2022 Mar 31;13(3):286. PMID: 35361778
J Med Chem

Discovery of Potent OTUB1/USP8 Dual Inhibitors Targeting Proteostasis in Non-Small-Cell Lung Cancer. [Abstract]

2022 Oct 27;65(20):13645-13659. PMID: 36221183
Cancer Cell Int

USP8 promotes the tumorigenesis of intrahepatic cholangiocarcinoma via stabilizing OGT. [Abstract]

2024 Jul 7;24(1):238. PMID: 38973004
J Invest Dermatol

The Identification of Potential Therapeutic Targets for Cutaneous Squamous Cell Carcinoma. [Abstract]

2020 Jun;140(6):1154-1165.e5. PMID: 31705877

DUB-IN-3 purchased from MedChemExpress. Usage Cited in: J Invest Dermatol. 2020 Jun;140(6):1154-1165.e5. [Abstract]

Western blot analysis of SCCRDEB4 cells treated with the USP8 inhibitor DUBs-IN-3 for 24 hours.
Mediators Inflamm

GPR34 Stabilized by Deubiquitinase USP8 Suppresses Ferroptosis of ATC. [Abstract]

2025 Aug 18:2025:5576056. PMID: 40862294
Arch Pharm (Weinheim)

Synthesis and structure-activity relationships of USP48 deubiquitinylase inhibitors. [Abstract]

2023 Jul;356(7):e2200661. PMID: 37196427

Solvente y solubilidad

In Vitro:

DMSO : 25 mg/mL (87.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4809 mL

17.4046 mL

34.8092 mL

5 mM

0.6962 mL

3.4809 mL

6.9618 mL

10 mM

0.3481 mL

1.7405 mL

3.4809 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (277 KB)
SDS (544 KB)
- English - EN (544 KB)
- Français - FR (544 KB)
- Deutsch - DE (544 KB)
- Norwegian - NO (544 KB)
- Español - ES (544 KB)
- Swedish - SV (544 KB)
- Italian - IT (544 KB)
- Korean - KR (544 KB)
- Portuguese - PT (544 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Colombo M, et al. Synthesis and biological evaluation of 9-oxo-9H-indeno[1,2-b]pyrazine-2,3-dicarbonitrile analogues as potential inhibitors of deubiquitinating enzymes. ChemMedChem. 2010 Apr 6;5(4):552-8. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4809 mL	17.4046 mL	34.8092 mL	87.0231 mL
	5 mM	0.6962 mL	3.4809 mL	6.9618 mL	17.4046 mL
	10 mM	0.3481 mL	1.7405 mL	3.4809 mL	8.7023 mL
	15 mM	0.2321 mL	1.1603 mL	2.3206 mL	5.8015 mL
	20 mM	0.1740 mL	0.8702 mL	1.7405 mL	4.3512 mL
	25 mM	0.1392 mL	0.6962 mL	1.3924 mL	3.4809 mL
	30 mM	0.1160 mL	0.5802 mL	1.1603 mL	2.9008 mL
	40 mM	0.0870 mL	0.4351 mL	0.8702 mL	2.1756 mL
	50 mM	0.0696 mL	0.3481 mL	0.6962 mL	1.7405 mL
	60 mM	0.0580 mL	0.2901 mL	0.5802 mL	1.4504 mL
	80 mM	0.0435 mL	0.2176 mL	0.4351 mL	1.0878 mL

Keywords

DUB-IN-3

7 Cited Publications

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DUB-IN-3

Anti-Cardiovascular Disease Compound Library

Ubiquitination Compound Library

Neurodegenerative Disease-related Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Publications Citing Use of MedChemExpress (MCE) DUB-IN-3

Ver todos los productos específicos de isoformas Deubiquitinase

Actividad biológica

Chemical Information

Publications (7)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

DUB-IN-3

Anti-Cardiovascular Disease Compound Library

Ubiquitination Compound Library

Neurodegenerative Disease-related Compound Library

Metabolic Enzyme Compound Library

Protease Inhibitor Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

Immunology/Inflammation Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Publications Citing Use of MedChemExpress (MCE) DUB-IN-3

Ver todos los productos específicos de isoformas Deubiquitinase

Actividad biológica

Chemical Information

Publications (7)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

DUB-IN-3 Related Classifications

HY-50737 Related Classifications