Engeletin
Based on 9 publication(s) in Google Scholar
Engeletin is a flavanonol glycoside isolated from Smilax glabra Roxb. , inhibits NF-κB signaling-pathway activation, and possesses anti-inflammatory, analgesic, diuresis, detumescence, and antibiosis effects.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.86%
- No. CAS: 572-31-6
- Fòrmula: C21H22O10
- Peso molecular:434.39
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Engeletin
More- Acta Pharm Sin B. 2021 Jan;11(1):143-155. [Abstract]
- Food Chem. 2025 Dec 30:497:146992. [Abstract]
- Food Chem. 2025 Oct 15:489:144992. [Abstract]
- Cell Death Discov. 2022 Dec 16;8(1):493. [Abstract]
- Ind Crops Prod. 2025 Dec 11;239:122458.
- J Inflamm Res. 2021 Mar 9:14:745-760. [Abstract]
- Curr Issues Mol Biol. 2025 Jul 10;47(7):535. [Abstract]
- SSRN. 2025 Dec 17.
- Journal of fish pathology. 2025 Jun 30;38(1):11-29.
Customer Validation & Images
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WB
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Cell Proliferation/Viability Assay
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RT-PCR
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Flow Cytometry
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IF
Actividad biológica
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NF-κB |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 572-31-6
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Appearance Solid
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Peso molecular 434.39
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Fòrmula C21H22O10
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Color White to yellow
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SMILES
OC1=CC(O)=C(C([C@H](O[C@@]2([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O2)[C@@H](C3=CC=C(O)C=C3)O4)=O)C4=C1
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (9)
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Journal Impact Factor
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Most Recent
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Acta Pharm Sin B
Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]2021 Jan;11(1):143-155. PMID: 33532186 -
Food Chem
Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]2025 Dec 30:497:146992. PMID: 41285060 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 Oct 15:489:144992. PMID: 40466530 -
Cell Death Discov
Cysteine dioxygenase 1 attenuates the proliferation via inducing oxidative stress and integrated stress response in gastric cancer cells. [Abstract]2022 Dec 16;8(1):493. PMID: 36526626 -
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J Inflamm Res
Engeletin Protects Against TNF-α-Induced Apoptosis and Reactive Oxygen Species Generation in Chondrocytes and Alleviates Osteoarthritis in vivo. [Abstract]2021 Mar 9:14:745-760. PMID: 33727849
Engeletin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Mar 9:14:745-760. [Abstract]
Chondrocytes are pretreated with Engeletin (10 μM and 20 μM) for two hours, and then TNF-α (20 ng/mL) is added to the culture medium and incubated for another 24 hours. The protein expression levels of collagen II, aggrecan, MMP9 and MMP3 are determined by Western blotting.
Engeletin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Mar 9:14:745-760. [Abstract]
Chondrocytes were treated with DMSO or different concentrations of Engeletin (5, 10, 20, 40, 80 and 160 μM) for 24 or 48 hours. Then, cell viability was measured by CCK-8 assay.
Engeletin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Mar 9:14:745-760. [Abstract]
The relative mRNA expression levels of collagen II, aggrecan, and MMP9 were determined by qPCR treated with Engeletin (10, 20 μM).
Engeletin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Mar 9:14:745-760. [Abstract]
Chondrocytes were stained with Annexin V-FITC/PI and measured by flow cytometry to determine the apoptotic rate treated with Engeletin (10, 20 μM).
Engeletin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Mar 9:14:745-760. [Abstract]
Chondrocytes were stained with JC-1 and observed using a confocal laser scanning fluoroscope treated with Engeletin (10, 20 μM).
Engeletin purchased from MedChemExpress. Usage Cited in: J Inflamm Res. 2021 Mar 9:14:745-760. [Abstract]
Representative hematoxylin-eosin, safranin O/fast green and toluidine blue staining images of rat knee cartilage from different groups after four weeks of Engeletin (50 μg/100 μL) intervention.
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Curr Issues Mol Biol
2025 Jul 10;47(7):535. PMID: 40729004 -
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Solvente y solubilidad
DMSO : ≥ 100 mg/mL (230.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.79 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
Mice[1]
The mice are classified at random into the following six groups of 10 mice each to cause the endometritis model: blank group, LPS group, Engeletin (25, 50, and 100 mg/kg) + LPS groups, and Engeletin (100 mg/kg) group. Engeletin is solubilized by heated normal saline to give the final concentrations of 25, 50, and 100 mg/kg. Briefly, each uterus is infused with 50 μL of LPS (1 mg/mL) to induce endometritis. At 24 h after the instillation, Engeletin groups receive an intraperitoneal injection of diverse Engeletin concentrations (25, 50, and 100 mg/kg) three times (once every 6 h). The Engeletin group is given an intraperitoneal injection of Engeletin (100 mg/kg). The blank group receive the normal saline. Afterward, the mice are killed by CO2 inhalation. Uterus tissues are harvested and kept in −80°C[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.3021 mL | 11.5104 mL | 23.0208 mL | 57.5520 mL |
| 5 mM | 0.4604 mL | 2.3021 mL | 4.6042 mL | 11.5104 mL | |
| 10 mM | 0.2302 mL | 1.1510 mL | 2.3021 mL | 5.7552 mL | |
| 15 mM | 0.1535 mL | 0.7674 mL | 1.5347 mL | 3.8368 mL | |
| 20 mM | 0.1151 mL | 0.5755 mL | 1.1510 mL | 2.8776 mL | |
| 25 mM | 0.0921 mL | 0.4604 mL | 0.9208 mL | 2.3021 mL | |
| 30 mM | 0.0767 mL | 0.3837 mL | 0.7674 mL | 1.9184 mL | |
| 40 mM | 0.0576 mL | 0.2878 mL | 0.5755 mL | 1.4388 mL | |
| 50 mM | 0.0460 mL | 0.2302 mL | 0.4604 mL | 1.1510 mL | |
| 60 mM | 0.0384 mL | 0.1918 mL | 0.3837 mL | 0.9592 mL | |
| 80 mM | 0.0288 mL | 0.1439 mL | 0.2878 mL | 0.7194 mL | |
| 100 mM | 0.0230 mL | 0.1151 mL | 0.2302 mL | 0.5755 mL |
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