ML349
Based on 8 publication(s) in Google Scholar
ML349 is a potent and specific acyl protein thioesterase 2(APT2)/lysophospholipase 2 (LYPLA2) inhibitor with a Ki of 120 nM. ML349 is also an inhibitor of LYPLA2 with an IC50 of 144 nM.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.90%
- No. CAS: 890819-86-0
- Fòrmula: C23H22N2O4S2
- Peso molecular:454.56
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Almacenamiento:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) ML349
More- Nat Cancer. 2023 Jun;4(6):829-843. [Abstract]
- Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]
- Adv Sci (Weinh). 2026 Mar 26:e19791. [Abstract]
- EMBO J. 2025 May;44(9):2596-2619. [Abstract]
- Cell Rep. 2026 Jan 24;45(2):116910. [Abstract]
- Sci Signal. 2025 May 27;18(888):eadr2008. [Abstract]
- J Virol. 2026 Jun 18:e0063826. [Abstract]
- Virology. 2025 Jun 25:610:110614. [Abstract]
Customer Validation & Images
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Bio/Physico-chemical Assay
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Ver todos los productos específicos de isoformas Phospholipase
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Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK-293T | IC50 |
>10 μM
Compound: ML349
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Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged PDXK expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay
Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged PDXK expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay
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[PMID: 28197315] |
| HEK-293T | IC50 |
1.1 μM
Compound: ML349
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Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay
Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay
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[PMID: 28197315] |
ML349 is an inhibitor of acyl protein thioesterase 1 and 2 (APT-1 and APT-2) with Kis of >10000 and 120±20 nM, respectively[1].
ML349 is also an inhibitor of both LYPLA1 and LYPLA2 with IC50s of >3000 and 144 nM, respectivley[2].
ML348 and ML349 do not decrease cell viability, but they lead to a slight activation of AKT in NRAS mutant cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 890819-86-0
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Appearance Solid
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Peso molecular 454.56
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Fòrmula C23H22N2O4S2
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Color White to yellow
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SMILES
COC1=CC=C(N2CCN(C(C3=CC4=C(C(C=CC=C5)=C5S(C4)(=O)=O)S3)=O)CC2)C=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (8)
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Journal Impact Factor
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Most Recent
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Nat Cancer
Scribble mis-localization induces adaptive resistance to KRAS G12C inhibitors through feedback activation of MAPK signaling mediated by YAP-induced MRAS. [Abstract]2023 Jun;4(6):829-843. PMID: 37277529 -
Nat Commun
Palmitoylation of TBK1 enhances the type I interferon signaling and strengthens anti-malarial immunity in mice. [Abstract]2025 Nov 18;16(1):10109. PMID: 41253837
ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]
PEMs were treated with ML349 (0-15 μM) at the indicated concentrations for 12 h. Cell lysates were collected for ABE assay and immunoblot analysis. This was repeated n = 3 independent times with similar results.
ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]
HEK293T cells were transfected with a luciferase reporter for IFN-β-luc and FLAG-TBK1 for 24 h, followed by treatment with ML349 (0-12 h) at different time points. Cell lysates were collected for luciferase reporter assays (n = 3 biological replicates).
ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]
PEMs were treated with ML349 (10 μM) for 12 h, then stimulated with gDNA at the indicated time points. Cell lysates were collected for immunoblot.
ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]
PEMs were treated with ML349 (10 μM) for 12 h, then stimulated with gDNA at the indicated time points. Cell lysates were collected for RT-qPCR analysis.
ML349 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 18;16(1):10109. [Abstract]
PEMs were treated with ML349 (0-15 μM) at indicated concentrations for 12 h. Cell lysates were collected for immunoblot analysis. This was repeated n = 3 independent times with similar results.
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Adv Sci (Weinh)
SMAD4 Palmitoylation Drives a Metabolic-Transcriptional Circuit to Promote Tumorigenesis and Confers Radiosensitivity in Pancreatic Cancer. [Abstract]2026 Mar 26:e19791. PMID: 41885390 -
EMBO J
S-palmitoylation modulates ATG2-dependent non-vesicular lipid transport during starvation-induced autophagy. [Abstract]2025 May;44(9):2596-2619. PMID: 40128367 -
Cell Rep
2026 Jan 24;45(2):116910. PMID: 41581148 -
Sci Signal
SMAD2 S-palmitoylation promotes its linker region phosphorylation and TH17 cell differentiation in a mouse model of multiple sclerosis. [Abstract]2025 May 27;18(888):eadr2008. PMID: 40424363 -
J Virol
Palmitoylation of CLDN12 regulated by ZDHHC7 and APT1/2 promotes hepatitis C virus entry. [Abstract]2026 Jun 18:e0063826. PMID: 42312836 -
Virology
2025 Jun 25:610:110614. PMID: 40582294
Solvente y solubilidad
DMSO : 25 mg/mL (55.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2 mg/mL (4.40 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocolo
Cells are plated in 96-well plates with a density of 4000 to 8000 cells per well and incubated for 24 h at 37°C with 5% CO2. Then cells are treated with increasing drug (including ML349) concentrations and their combinations. Cell viability is measured with the cell viability assay according to the manufacturer's protocol[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureza y Documentación
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Ficha de datos (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Won SJ, et al. Molecular Mechanism for Isoform-Selective Inhibition of Acyl Protein Thioesterases 1 and 2 (APT1 and APT2). ACS Chem Biol. 2016 Dec 16;11(12):3374-3382. [Content Brief]
[2]. Adibekian A, et al. Characterization of a Selective, Reversible Inhibitor of Lysophospholipase 2 (LYPLA2). [Content Brief]
[3]. Vujic I, et al. Acyl protein thioesterase 1 and 2 (APT-1, APT-2) inhibitors palmostatin B, ML348 and ML349 have different effects on NRAS mutant melanoma cells. Oncotarget. 2016 Feb 9;7(6):7297-306. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1999 mL | 10.9996 mL | 21.9993 mL | 54.9982 mL |
| 5 mM | 0.4400 mL | 2.1999 mL | 4.3999 mL | 10.9996 mL | |
| 10 mM | 0.2200 mL | 1.1000 mL | 2.1999 mL | 5.4998 mL | |
| 15 mM | 0.1467 mL | 0.7333 mL | 1.4666 mL | 3.6665 mL | |
| 20 mM | 0.1100 mL | 0.5500 mL | 1.1000 mL | 2.7499 mL | |
| 25 mM | 0.0880 mL | 0.4400 mL | 0.8800 mL | 2.1999 mL | |
| 30 mM | 0.0733 mL | 0.3667 mL | 0.7333 mL | 1.8333 mL | |
| 40 mM | 0.0550 mL | 0.2750 mL | 0.5500 mL | 1.3750 mL | |
| 50 mM | 0.0440 mL | 0.2200 mL | 0.4400 mL | 1.1000 mL |
ML349 Related Classifications
HY-100737 Related Classifications
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