Referencia número: HY-110204

Heclin

3 Cited Publications

Revisión del cliente

Based on 3 publication(s) in Google Scholar

Heclin is a HECT E3 ubiquitin ligases inhibitor. Heclin inhibits Smurf2, Nedd4, WWP1 (IC₅₀ values are 6.8, 6.3, 6.9 μM) and can be used for the research of gastric cancer.

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Pureza: 98.00%
No. CAS: 890605-54-6
Fòrmula: C₁₇H₁₇NO₃
Peso molecular:283.32

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Heclin Chemical Structure

No. CAS: 890605-54-6

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Actividad biológica
- Descripciòn
- In Vitro
Chemical Information
Publications (3)
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

Heclin is a HECT E3 ubiquitin ligases inhibitor. Heclin inhibits Smurf2, Nedd4, WWP1 (IC₅₀ values are 6.8, 6.3, 6.9 μM) and can be used for the research of gastric cancer^[1]^[2]^[3].

Ver todos los productos específicos de isoformas Akt :

In Vitro

Heclin (10 μM, 1-5 days) inhibits cell viability and IGF1 signaling of BGC803 and MKN45 cell lines^[1].
Heclin (7 μM, 1 h) ameliorates epinecidin-1-mediated MyD88 degradation in Raw264.7 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

BGC803 and MKN45 cells
Concentration:

10 μM
Incubation Time:

48 h
Result:

Reduced phosphorylation of Akt.

Chemical Information

No. CAS 890605-54-6
Appearance Solid
Peso molecular 283.32
Fòrmula C₁₇H₁₇NO₃
Color Off-white to light brown
SMILES

CC(C(C=C1)=CC=C1NC(/C=C/C2=CC=C(CC)O2)=O)=O
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

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Journal Impact Factor

Mol Cell

SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation. [Abstract]

2023 Dec 7;83(23):4352-4369.e8. PMID: 38016474
Nat Chem Biol

µMap proximity labeling in living cells reveals stress granule disassembly mechanisms. [Abstract]

2025 Apr;21(4):490-500. PMID: 39215100

Heclin purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2025 Apr;21(4):490-500. [Abstract]

Heclin (25 μM; 1 h) delayed stress granule disassembly in cell lines including SHSY5Y and HeLa.

Heclin purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2025 Apr;21(4):490-500. [Abstract]

Heclin (25 μM; 1 h) caused delayed SG disassembly in HEK293 cells at 120 min recovery.

Heclin purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2025 Apr;21(4):490-500. [Abstract]

Heclin (25 μM; 1 h) caused heclin-treated HEK293 cells to display ～2-h delays in clearance.

Heclin purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2025 Apr;21(4):490-500. [Abstract]

Heclin (25 μM; 1 h) decreased the overlap of K63-specific ubiquitination signals within granule areas of HEK293 cells.
SSRN

2025 Nov 5.

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (352.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5296 mL

17.6479 mL

35.2958 mL

5 mM

0.7059 mL

3.5296 mL

7.0592 mL

10 mM

0.3530 mL

1.7648 mL

3.5296 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (273 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Wang K, et al. Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer [J]. Oncogene, 2023, 42(14): 1072-1087. [Content Brief]

[2]. Su B C, Chen J Y. Antimicrobial peptide epinecidin-1 modulates MyD88 protein levels via the proteasome degradation pathway [J]. Marine Drugs, 2017, 15(11): 362. [Content Brief]

[3]. Mund T, et al. Peptide and small molecule inhibitors of HECT-type ubiquitin ligases [J]. Proceedings of the National Academy of Sciences, 2014, 111(47): 16736-16741. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5296 mL	17.6479 mL	35.2958 mL	88.2394 mL
	5 mM	0.7059 mL	3.5296 mL	7.0592 mL	17.6479 mL
	10 mM	0.3530 mL	1.7648 mL	3.5296 mL	8.8239 mL
	15 mM	0.2353 mL	1.1765 mL	2.3531 mL	5.8826 mL
	20 mM	0.1765 mL	0.8824 mL	1.7648 mL	4.4120 mL
	25 mM	0.1412 mL	0.7059 mL	1.4118 mL	3.5296 mL
	30 mM	0.1177 mL	0.5883 mL	1.1765 mL	2.9413 mL
	40 mM	0.0882 mL	0.4412 mL	0.8824 mL	2.2060 mL
	50 mM	0.0706 mL	0.3530 mL	0.7059 mL	1.7648 mL
	60 mM	0.0588 mL	0.2941 mL	0.5883 mL	1.4707 mL
	80 mM	0.0441 mL	0.2206 mL	0.4412 mL	1.1030 mL
	100 mM	0.0353 mL	0.1765 mL	0.3530 mL	0.8824 mL

Keywords

Heclin

3 Cited Publications

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Heclin

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

PI3K/Akt/mTOR Compound Library

Stem Cell Signaling Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

Covalent Screening Library

Oxygen Sensing Compound Library

Ubiquitination Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Glutamine Metabolism Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Angiogenesis-Related Compound Library

Glucose Metabolism Compound Library

Anti-Liver Cancer Compound Library

Pain-Related Compound Library

Metabolic Enzyme Compound Library

Cysteine Targeted Covalent Library

Multi-Target Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Amino Acid Metabolism Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Heclin

Ver todos los productos específicos de isoformas Akt

Actividad biológica

Chemical Information

Publications (3)

Solvente y solubilidad

Pureza y Documentación

Referencias

Help & FAQs

Complete Stock Solution Preparation Table

Heclin Related Classifications

HY-110204 Related Classifications