IBI323
IBI323 is a PD-L1/LAG-3 dual inhibitory antibody with Kd values of 0.0132 nM and 0.671 nM, respectively. IBI323 blocks the interaction of PD-L1 with PD-1 and CD80, as well as the interaction of LAG-3 with MHC-II. IBI323 mediates cross-linking of PD-L1-positive and LAG-3-positive cells, enhances T cell activation and cytokine secretion, and increases tumor-specific T cells in tumors and blood. IBI323 has a LALA mutation that reduces Fc-mediated effector function and inhibits tumor growth in humanized mouse models. IBI323 can be used for research on melanoma and colon cancer.
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
Descripciòn
Species Reactivity
Human
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| CHO | IC50 |
39.75 nM
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Blocking of LAG-3/MHC-II interaction in CHO cells overexpressing human MHC class II assessed by FACS after 30 min incubation at 4°C.
Blocking of LAG-3/MHC-II interaction in CHO cells overexpressing human MHC class II assessed by FACS after 30 min incubation at 4°C.
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34239776 |
In Vitro
IBI323 (incubated at 4°C for 30 min) binds activated primary human CD4+ T cells in a dose-dependent manner[1].
IBI323 (incubated at 4°C for 30 min) blocks PD-L1/PD-1 and PD-L1/CD80 interactions on CHO-S-hPD-L1 cells, with IC50 values of 23.99 nM and 14.96 nM, respectively[1].
IBI323 (incubated at 4°C for 30 min) blocks the LAG-3/MHC-II interaction with an IC50 of 39.75 nM[1].
IBI323 (6 h) activates LAG-3/MHC-II reporter Jurkat cells by blocking the LAG-3/MHC-II interaction[1].
IBI323 (incubated at 4°C for 30 min to room temperature for 1 h) mediates bridging between PD-L1+ MDA-MB-231 cells and LAG-3+ Jurkat T cells[1].
IBI323 (200 nM; 4 days) enhances IFN-γ and IL-2 secretion in a dose-dependent manner in human DC/CD4+ T cell mixed leukocyte reaction[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
IBI323 (3.5-11.6 mg/kg; intraperitoneal injection; on days 1, 7, 10, 13, and 16 after tumor cell implantation) significantly inhibited the growth of A375 melanoma in humanized NOG mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6-CD274tm1(hCD274)LAG3tm1(hLAG3) (female)[1]
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Dosage:5.8 mg/kg; 1 mg/kg
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Administration:i.p. on days 6, 10, 14, 17, and 21 post implantation
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Result:Inhibited tumor growth in a dose-dependent manner.
Showed a significant reduction in tumor volume relative to the IgG group.
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Animal Model:NOG (strain: 408; female)[1]
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Dosage:3.5 mg/kg; 11.6 mg/kg
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Administration:i.p. on days 1, 7, 10, 13, and 16 after tumor cell implantation
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Result:Significantly inhibited A375 tumor growth at both 3.5 mg/kg and 11.6 mg/kg doses.
Target
LAG-3 & PD-L1
Conjugated
Unconjugated
Aplicación
ELISA, FACS, Functional assay
Chemical Information
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SMILES
[IBI323]
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
IBI323 Related Classifications
HY-P992546 Related Classifications
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