L-Quebrachitol
Based on 1 Customer Validation
L-Quebrachitol is a methoxy analog of inositol that can be isolated from plants. L-Quebrachitol has free-radical scavenging, gastroprotection, anti-platelet aggregation and anti-diabetic activity. L-Quebrachitol promotes osteoblastogenesis by uppregulation of BMP-2, runt-related transcription factor-2 (Runx2), MAPK (ERK, JNK, p38α), and Wnt/β-catenin signaling pathway.
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- Pureza : 99.51%
- No. CAS: 642-38-6
- Fòrmula: C7H14O6
- Peso molecular:194.18
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
In Vitro
L-Quebrachitol (0.01-100 μg/mL , 24-72 h) enhances the cell viability of pre-osteoblastic MC3T3-E1 cells[1].
L-Quebrachitol ( 0.001-10 μg/mL , 48 h) promotes cell DNA synthesis in MC3T3-E1 cells[1].
L-Quebrachitol ( 0.001-10 μg/mL , 48 h) up-regulates mRNA expression of BMP-2, Runx2, osteogenesis markers and increases expression of a gene involved in the MAPK and Wnt/β-Catenin in MC3T3-E1 cells[1].
L-Quebrachitol (0-1.5 mg/mL , 24 h) significantly decreases the cell viability in HepG2 cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:pre-osteoblastic MC3T3-E1 cells
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Concentration:0.01-100 μg/mL
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Incubation Time:24-72 h
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Result:Significantly increased cell proliferation in a broadly concentration-dependent manner from 0.01 to 100 µg/mL in pre-osteoblastic MC3T3-E1 cells.
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Cell Line:MC3T3-E1 cells
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Concentration:0.001-10 μg/mL
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Incubation Time:48 h
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Result:Significantly decreased the proportion of cells in the G0/G1 phase, whereas increased the percentage of cells in S phase.
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Cell Line:pre-osteoblastic MC3T3-E1 cells
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Concentration:0.001-10 μg/mL
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Incubation Time:24-72 h
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Result:Significantly increased the expression of osteocalcin, osteopontin, runt-related transcription factor-2, and alkaline phosphatase genes after 24 h exposure.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 642-38-6
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Appearance Solid
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Peso molecular 194.18
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Fòrmula C7H14O6
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Color White to off-white
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SMILES
CO[C@H]1[C@@H]([C@H]([C@H](O)[C@H](O)[C@H]1O)O)O
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvente y solubilidad
In Vitro:
DMSO : 125 mg/mL (643.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (514.99 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (10.71 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (10.71 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocolo
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Nuclear Protein Extraction (High-Salt/Hypotonic Fractionation)
The high-salt/hypotonic fractionation method for nuclear protein extraction is based on the differential solubility of cellular components. Cytoplasmic proteins are extracted first using a hypotonic buffer that causes cell swelling and membrane rupture, followed by centrifugation to separate the cytoplasmic supernatant from the nuclear pellet. The nuclear pellet is then subjected to high-salt extraction (e. g. , 0. 4 M (NH4)2SO4 or 1 M NaCl) to solubilize tightly bound nuclear matrix proteins, including transcription factors, histones, and structural proteins associated with chromatin and the nuclear scaffold. This approach allows for the isolation of both soluble cytoplasmic proteins and salt-resistant nuclear proteins while minimizing cross-contamination.
Pureza y Documentación
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Ficha de datos (283 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 5.1499 mL | 25.7493 mL | 51.4986 mL | 128.7465 mL |
| 5 mM | 1.0300 mL | 5.1499 mL | 10.2997 mL | 25.7493 mL | |
| 10 mM | 0.5150 mL | 2.5749 mL | 5.1499 mL | 12.8747 mL | |
| 15 mM | 0.3433 mL | 1.7166 mL | 3.4332 mL | 8.5831 mL | |
| 20 mM | 0.2575 mL | 1.2875 mL | 2.5749 mL | 6.4373 mL | |
| 25 mM | 0.2060 mL | 1.0300 mL | 2.0599 mL | 5.1499 mL | |
| 30 mM | 0.1717 mL | 0.8583 mL | 1.7166 mL | 4.2916 mL | |
| 40 mM | 0.1287 mL | 0.6437 mL | 1.2875 mL | 3.2187 mL | |
| 50 mM | 0.1030 mL | 0.5150 mL | 1.0300 mL | 2.5749 mL | |
| 60 mM | 0.0858 mL | 0.4292 mL | 0.8583 mL | 2.1458 mL | |
| 80 mM | 0.0644 mL | 0.3219 mL | 0.6437 mL | 1.6093 mL | |
| 100 mM | 0.0515 mL | 0.2575 mL | 0.5150 mL | 1.2875 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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