Mutanolysin
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Mutanolysin is a bacteriolytic agent. Mutanolysin is a muralytic enzyme that can prevent hepatic injury. Mutanolysin can digest the cell wall of S. mutans BHT and shows antibacterial activity. Mutanolysin reduces TNF-α production in isolated Kupffer cells stimulated with peptidoglycan-polysaccharide (PG-APS). Mutanolysin can be used for the researches of infection, inflammation and hepatic injury.
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- No. CAS: 55466-22-3
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
Descripciòn
In Vitro
Mutanolysin (200 μg; 36 h) digests PG-APS to reduce TNFα production by purified Lewis rat Kupffer cells by 75%, from 486 pg/mL to 145 pg/mL[1].
Mutanolysin (20 μg/mL, 90 min) lyses and kills live S. mutans AHT and BHT cells after 90 min of incubation, with 99.999% killing efficacy against S. mutans AHT and 99.0% killing efficacy against S. mutans BHT[2].
Mutanolysin exhibits broad lytic activity against gram-positive oral pathogens including S. mutans, A. viscosus, and L. acidophilus, but is inactive against S. aureus, gram-negative bacteria, and yeast[2].
Mutanolysin lyses S. mutans BHT cells by rupturing cell walls[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Mutanolysin (0.4 mg; i.v.; single dose) completely prevents the development of chronic erosive arthritis, resolves acute arthritis within 14 days, and eliminates granulomatous lesions in visceral tissues[3].
Mutanolysin (0.4 mg; i.v.; single dose; administered 24 h or 3 days post-PG-APS) reduces acute arthritis severity and completely prevents the development of chronic erosive arthritis[3].
Mutanolysin (0.4 mg; i.v.; single dose; administered 14 days post-PG-APS) significantly reduces the severity of chronic joint disease, with only 33% of treated rats developing recurrent disease[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Lewis rats (female, 100-120 g at time of PG-APS injection, arthritis model via intraperitoneal injection of arthropathic PG-APS)[3]
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Dosage:0.4 mg
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Administration:i.v.; single dose
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Result:Prevented development of chronic erosive arthritis.
Resolved mild acute arthritis within 14 days.
Eliminated granulomatous lesions in liver, spleen, and mediastinal lymph nodes.
Reduced transient limb edema, which peaked at 2-3 hours and subsided by 9 hours.
Showed normal-appearing joint tissue with intact cartilage, no exudate, and only mild residual focal synovial scarring and lining cell hyperplasia at 41 days post-injection.
Demonstrated no chronic erosive arthritis on radiographic assessment.
Maintained tissue PG-APS levels in hind limbs, spleen, liver, and blood equal to or exceeding PBS-treated controls at days 2, 5, and 36 post-injection.
Specific Activity
≥ 4000 U/mg protein
Unit Definition
One unit is defined as the amount of enzyme that will produce a change in absorbance at 600 nm of 0.01 per minute at pH 6.0 at 37°C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate
Chemical Information
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No. CAS 55466-22-3
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Appearance Solid
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Color White to off-white
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SMILES
[Mutanolysin]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
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Ficha de datos (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Lichtman SN, et al. Degradation of endogenous bacterial cell wall polymers by the muralytic enzyme mutanolysin prevents hepatobiliary injury in genetically susceptible rats with experimental intestinal bacterial overgrowth. J Clin Invest. 1992;90(4):1313-1322. [Content Brief]
[2]. Yokogawa K, et al. Mutanolysin, bacteriolytic agent for cariogenic Streptococci: partial purification and properties. Antimicrob Agents Chemother. 1974;6(2):156-165. [Content Brief]
[3]. Janusz MJ, et al. Treatment of experimental erosive arthritis in rats by injection of the muralytic enzyme mutanolysin. J Exp Med. 1984;160(5):1360-1374. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Mutanolysin Related Classifications
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