Phosphoramide mustard cyclohexanamine
Based on 9 publication(s) in Google Scholar
Phosphoramide mustard cyclohexanamine is a biologically active metabolite of Cyclophosphamide (HY-17420), with anticancer activitiy. Phosphoramide mustard cyclohexanamine induces DNA damage.
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- Pureza: 98.0%
- No. CAS: 1566-15-0
- Fòrmula: C10H24Cl2N3O2P
- Peso molecular:320.20
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Almacenamiento:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
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Publications Citing Use of MedChemExpress (MCE) Phosphoramide mustard cyclohexanamine
More- Sci Adv. 2026 Apr 3;12(14):eaec8684. [Abstract]
- Int J Nanomedicine. 2025 Aug 22:20:10195-10212. [Abstract]
- Stem Cell Res Ther. 2024 Apr 5;15(1):97. [Abstract]
- Sci Rep. 2024 Aug 16;14(1):19008. [Abstract]
- J Funct Foods. 2025 Jun.
- Climacteric. 2025 Apr;28(2):200-211. [Abstract]
- SSRN. 2026 Jun 21.
- Tufts University. 2025.
- SSRN. 2024 Mar 21.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
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WB
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Cell Imaging/Staining
Actividad biológica
Phosphoramide mustard cyclohexanamine causes cytotoxicity through forming cross-linked DNA adducts which inhibit DNA strand separation during replication[1].
Phosphoramide mustard cyclohexanamine (3-6 μM; 48 hours) reduces cell viability in rat spontaneously immortalized granulosa cells (SIGCs)[1].
Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) induces DNA adduct formation and ovarian DNA damage[1].
Phosphoramide mustard cyclohexanamine (3-6 μM; 24-48 hours) increases DNA damage responses (DDR) gene mRNA expression levels and DDR proteins[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SIGCs
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Concentration:0.5 μM, 1 μM, 3 μM, 6 μM
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Incubation Time:48 hours
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Result:Reduced cell viability at concentrations of 3 μM and higher.
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Cell Line:SIGCs
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Concentration:3 μM, 6 μM
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Incubation Time:24 hours, 48 hours
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Result:Increased DDR gene mRNA expression levels.
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Cell Line:SIGCs
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Concentration:3 μM, 6 μM
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Incubation Time:24 hours, 48 hours
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Result:Generally increased DDR proteins.
Phosphoramide mustard cyclohexanamine exhibits terminal elimination half-lives (rat 15.1 min) following intravenous administration (rat 59.4 mg/kg)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rat, subcutaneously implanted Walker 256 carcinosarcoma tumor[2]
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Dosage:2.1 mg/kg, 4.8 mg/kg, 10.4 mg/kg, 20.7 mg/kg
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Administration:Intraperitoneal injection, once daily, for 5 consecutive days
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Result:Required to produce 50% inhibition of subcutaneous tumor growth with dose of 12 mg/kg.
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Animal Model:Rats[2]
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Dosage:86.0 mg/kg (Pharmacokinetic Analysis)
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Administration:Intravenous injection
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Result:T1/2 (15.1 min).
Chemical Information
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No. CAS 1566-15-0
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Appearance Solid
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Peso molecular 320.20
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Fòrmula C10H24Cl2N3O2P
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Color White to off-white
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SMILES
O=P(N(CCCl)CCCl)(N)O.NC1CCCCC1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (9)
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Journal Impact Factor
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Most Recent
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Sci Adv
Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC. [Abstract]2026 Apr 3;12(14):eaec8684. PMID: 41931605 -
Int J Nanomedicine
Exosomes Derived From Human Mesenchymal Stem Cells Mitigate Follicular Interstitial Cell Ferroptosis via the miR-26a-5p/PTEN/GPX4 Axis in Rats with Chemotherapy-Induced Premature Ovarian Insufficiency. [Abstract]2025 Aug 22:20:10195-10212. PMID: 40873678 -
Stem Cell Res Ther
Pyrroloquinoline quinone promotes human mesenchymal stem cell-derived mitochondria to improve premature ovarian insufficiency in mice through the SIRT1/ATM/p53 pathway. [Abstract]2024 Apr 5;15(1):97. PMID: 38581065 -
Sci Rep
hUMSCs restore ovarian function in POI mice by regulating GSK3β-mediated mitochondrial dynamic imbalances in theca cells. [Abstract]2024 Aug 16;14(1):19008. PMID: 39152165 -
Phosphoramide mustard cyclohexanamine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.
Phosphoramide mustard cyclohexanamine (PM) (3–120 μM; 48 h) significantly inhibited Caco-2 cell viability compared with the control-treated group.
Phosphoramide mustard cyclohexanamine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.
Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) (PM) promoted the increase of MDA content in Caco-2 cells in a concentration-dependent manner.
Phosphoramide mustard cyclohexanamine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.
Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) significantly enhanced the red fluorescence intensity emitted by RhoNox-1 staining, indicating the specific accumulation of Fe2+ in Caco-2 cells.
Phosphoramide mustard cyclohexanamine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.
Phosphoramide mustard cyclohexanamine (PM) (15–120 μM; 48 h) treatment of Caco-2 cells caused overexpression of NCOA4 protein in a concentration-dependent manner.
Phosphoramide mustard cyclohexanamine purchased from MedChemExpress. Usage Cited in: J Funct Foods. 2025 Jun.
Phosphoramide mustard cyclohexanamine (PM) (30 μM; 48 h) induced autophagy in Caco-2 cells, as evidenced by increased autophagosomes and autophagolysosomes.
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Climacteric
Intravenous administration of mitochondria improves ovarian function by anti-apoptosis in the premature ovarian insufficiency model. [Abstract]2025 Apr;28(2):200-211. PMID: 39791362 -
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Solvente y solubilidad
H2O : ≥ 50 mg/mL (156.15 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (312.30 mM); Clear solution; Need ultrasonic
Pureza y Documentación
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Ficha de datos (281 KB)
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SDS (393 KB)
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- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Shanthi Ganesan, et al. Phosphoramide mustard exposure induces DNA adduct formation and the DNA damage repair response in rat ovarian granulosa cells. Toxicol Appl Pharmacol. 2015 Feb 1; 282(3): 252–258. [Content Brief]
[2]. S Genka, et al. Brain and plasma pharmacokinetics and anticancer activities of cyclophosphamide and phosphoramide mustard in the rat. Cancer Chemother Pharmacol. 1990;27(1):1-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.1230 mL | 15.6152 mL | 31.2305 mL | 78.0762 mL |
| 5 mM | 0.6246 mL | 3.1230 mL | 6.2461 mL | 15.6152 mL | |
| 10 mM | 0.3123 mL | 1.5615 mL | 3.1230 mL | 7.8076 mL | |
| 15 mM | 0.2082 mL | 1.0410 mL | 2.0820 mL | 5.2051 mL | |
| 20 mM | 0.1562 mL | 0.7808 mL | 1.5615 mL | 3.9038 mL | |
| 25 mM | 0.1249 mL | 0.6246 mL | 1.2492 mL | 3.1230 mL | |
| 30 mM | 0.1041 mL | 0.5205 mL | 1.0410 mL | 2.6025 mL | |
| 40 mM | 0.0781 mL | 0.3904 mL | 0.7808 mL | 1.9519 mL | |
| 50 mM | 0.0625 mL | 0.3123 mL | 0.6246 mL | 1.5615 mL | |
| 60 mM | 0.0521 mL | 0.2603 mL | 0.5205 mL | 1.3013 mL | |
| 80 mM | 0.0390 mL | 0.1952 mL | 0.3904 mL | 0.9760 mL | |
| 100 mM | 0.0312 mL | 0.1562 mL | 0.3123 mL | 0.7808 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Phosphoramide mustard cyclohexanamine Related Classifications
HY-137316A Related Classifications
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