DHX9 - DExH-box helicase 9 Gene

Also Known as LKP; RHA; DDX9; NDH2; NDHII

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 1660

About DHX9

Cytogenetic location: 1q25.3 Genomic coordinates (GRCh38): 1:182,839,347-182,887,982 (from NCBI)

This gene has 8 transcripts (splice variants), 199 orthologues and 18 paralogues. Ubiquitous expression in bone marrow (RPKM 54.5), testis (RPKM 36.3) and 25 other tissues.

Summary

This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.[provided by RefSeq, Feb 2010]

DHX9 Products (1)

mRNA	Protein	Name
NM_001357.5	NP_001348.2	ATP-dependent RNA helicase A

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables 3'-5' DNA helicase activity	IDA IDA: Inferred from direct assay	21561811	GOA
enables 3'-5' DNA/RNA helicase activity	IDA IDA: Inferred from direct assay	1537828	GOA
enables 3'-5' RNA helicase activity	IDA IDA: Inferred from direct assay	1537828	GOA
enables ATP binding	IDA IDA: Inferred from direct assay	11416126	GOA
enables ATP hydrolysis activity	IMP IMP: Inferred from mutant phenotype	11416126	GOA
enables DNA helicase activity	IMP IMP: Inferred from mutant phenotype	12711669	GOA
enables DNA replication origin binding	IDA IDA: Inferred from direct assay	24990949	GOA
enables RISC complex binding	IDA IDA: Inferred from direct assay	17531811	GOA
enables RNA binding	IDA IDA: Inferred from direct assay	1537828	GOA
enables RNA helicase activity	IDA IDA: Inferred from direct assay	28221134	GOA
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA IDA: Inferred from direct assay	11038348	GOA
enables RNA polymerase II complex binding	IDA IDA: Inferred from direct assay	11416126	GOA
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI IPI: Inferred from physical interaction	17303075	GOA
enables RNA polymerase binding	IDA IDA: Inferred from direct assay	9323138	GOA
enables RNA stem-loop binding	IDA IDA: Inferred from direct assay	11096080	GOA
enables chromatin DNA binding	IDA IDA: Inferred from direct assay	24049074	GOA
enables double-stranded DNA binding	IDA IDA: Inferred from direct assay	9111062	GOA
enables double-stranded RNA binding	IDA IDA: Inferred from direct assay	9111062	GOA
enables importin-alpha family protein binding	IDA IDA: Inferred from direct assay	16375861	GOA
enables mRNA binding	IDA IDA: Inferred from direct assay	10198287	GOA
enables mRNA binding	IMP IMP: Inferred from mutant phenotype	28221134	GOA
enables nucleoside triphosphate diphosphatase activity	IDA IDA: Inferred from direct assay	1537828	GOA
enables promoter-specific chromatin binding	IMP IMP: Inferred from mutant phenotype	28221134	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	9323138	GOA
enables regulatory region RNA binding	IDA IDA: Inferred from direct assay	28355180	GOA
enables ribosome binding	IDA IDA: Inferred from direct assay	16680162	GOA
enables sequence-specific mRNA binding	IDA IDA: Inferred from direct assay	9162007	GOA
enables siRNA binding	IDA IDA: Inferred from direct assay	23361462	GOA
enables single-stranded 3'-5' DNA helicase activity	IDA IDA: Inferred from direct assay	20669935	GOA
enables single-stranded DNA binding	IDA IDA: Inferred from direct assay	9111062	GOA
enables single-stranded RNA binding	IDA IDA: Inferred from direct assay	9111062	GOA
enables transcription coactivator activity	IMP IMP: Inferred from mutant phenotype	9323138	GOA
enables transcription coregulator activity	IMP IMP: Inferred from mutant phenotype	11416126	GOA
enables triplex DNA binding	IDA IDA: Inferred from direct assay	24049074	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in CRD-mediated mRNA stabilization	IDA IDA: Inferred from direct assay	19029303	GOA
involved in CRD-mediated mRNA stabilization	IMP IMP: Inferred from mutant phenotype	19029303	GOA
involved in DNA duplex unwinding	IDA IDA: Inferred from direct assay	9111062	GOA
involved in DNA duplex unwinding	IMP IMP: Inferred from mutant phenotype	12711669	GOA
involved in DNA-templated viral transcription	IDA IDA: Inferred from direct assay	11096080	GOA
involved in G-quadruplex DNA unwinding	IDA IDA: Inferred from direct assay	21561811	GOA
involved in RISC complex assembly	IMP IMP: Inferred from mutant phenotype	17531811	GOA
involved in RNA secondary structure unwinding	IDA IDA: Inferred from direct assay	1537828	GOA
involved in alternative mRNA splicing, via spliceosome	IMP IMP: Inferred from mutant phenotype	28221134	GOA
involved in cellular response to exogenous dsRNA	IMP IMP: Inferred from mutant phenotype	21957149	GOA
involved in cellular response to tumor necrosis factor	IMP IMP: Inferred from mutant phenotype	15355351	GOA
involved in miRNA-mediated post-transcriptional gene silencing	IMP IMP: Inferred from mutant phenotype	17531811	GOA
involved in negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	IDA IDA: Inferred from direct assay	19029303	GOA
involved in positive regulation of DNA repair	IMP IMP: Inferred from mutant phenotype	24049074	GOA
involved in positive regulation of DNA replication	IMP IMP: Inferred from mutant phenotype	24990949	GOA
involved in positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity	IMP IMP: Inferred from mutant phenotype	12711669	GOA
involved in positive regulation of NF-kappaB transcription factor activity	IMP IMP: Inferred from mutant phenotype	15355351	GOA
involved in positive regulation of RNA export from nucleus	IDA IDA: Inferred from direct assay	11402034	GOA
involved in positive regulation of cytoplasmic translation	IDA IDA: Inferred from direct assay	19029303	GOA
involved in positive regulation of cytoplasmic translation	IMP IMP: Inferred from mutant phenotype	16680162	GOA
involved in positive regulation of fibroblast proliferation	IMP IMP: Inferred from mutant phenotype	24990949	GOA
involved in positive regulation of interferon-alpha production	IMP IMP: Inferred from mutant phenotype	21957149	GOA
involved in positive regulation of interferon-beta production	IMP IMP: Inferred from mutant phenotype	21957149	GOA
involved in positive regulation of interleukin-6 production	IMP IMP: Inferred from mutant phenotype	20696886	GOA
involved in positive regulation of response to cytokine stimulus	IMP IMP: Inferred from mutant phenotype	21957149	GOA
involved in positive regulation of transcription by RNA polymerase II	IMP IMP: Inferred from mutant phenotype	9323138	GOA
involved in positive regulation of tumor necrosis factor production	IMP IMP: Inferred from mutant phenotype	20696886	GOA
involved in positive regulation of viral transcription	IDA IDA: Inferred from direct assay	11096080	GOA
involved in protein localization to cytoplasmic stress granule	IMP IMP: Inferred from mutant phenotype	24965446	GOA
involved in protein-containing complex assembly	IDA IDA: Inferred from direct assay	23361462	GOA
involved in regulation of cytoplasmic translation	IDA IDA: Inferred from direct assay	28355180	GOA
involved in regulation of mRNA processing	IDA IDA: Inferred from direct assay	28355180	GOA
involved in regulation of transcription by RNA polymerase II	IMP IMP: Inferred from mutant phenotype	9662397	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of CRD-mediated mRNA stability complex	IDA IDA: Inferred from direct assay	19029303	GOA
part of CRD-mediated mRNA stability complex	IPI IPI: Inferred from physical interaction	19029303	GOA
part of RISC complex	IDA IDA: Inferred from direct assay	17531811	GOA
part of RISC-loading complex	IMP IMP: Inferred from mutant phenotype	17531811	GOA
located in actin cytoskeleton	IDA IDA: Inferred from direct assay	11687588	GOA
located in centrosome	IDA IDA: Inferred from direct assay	17498979	GOA
located in cytoplasm	IDA IDA: Inferred from direct assay	9162007	GOA
located in cytoplasmic ribonucleoprotein granule	IDA IDA: Inferred from direct assay	10198287	GOA
located in cytosol	IDA IDA: Inferred from direct assay	19029303	GOA
located in nuclear body	IDA IDA: Inferred from direct assay	28221134	GOA
located in nuclear stress granule	IDA IDA: Inferred from direct assay	10198287	GOA
located in nuclear stress granule	IMP IMP: Inferred from mutant phenotype	24965446	GOA
located in nucleoplasm	IDA IDA: Inferred from direct assay	28221134	GOA
located in nucleus	IDA IDA: Inferred from direct assay	9162007	GOA
located in perichromatin fibrils	IDA IDA: Inferred from direct assay	10198287	GOA
part of protein-containing complex	IDA IDA: Inferred from direct assay	11687588	GOA
part of ribonucleoprotein complex	IDA IDA: Inferred from direct assay	11402034	GOA
part of ribonucleoprotein complex	IMP IMP: Inferred from mutant phenotype	28221134	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

DHX9 Protein Structure

dsrm

DEAD

Helicase_C

HA2

OB_NTP_bind

0
200
400
600
800
1000
1200
1270 a.a.

Protein Preferred Names	Protein Names
ATP-dependent RNA helicase A	DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9

DHX9 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	DHX9	Q08211	HNRNPA2B1	Homo sapiens	P22626	Anti Bait CoIP	18519039
Intra	DHX9	Q08211	HNRNPA2B1	Homo sapiens	P22626	Anti Tag CoIP	35271311
Intra	DHX9	Q08211	YBX1	Homo sapiens	P67809	Anti Bait CoIP	21849455
Intra	DHX9	Q08211	YBX1	Homo sapiens	P67809	Anti Tag CoIP	35271311
Intra	DHX9	Q08211	YBX1	Homo sapiens	P67809	Anti Bait CoIP	23986595
Intra	DHX9	Q08211	XPO1	Homo sapiens	O14980	Anti Bait CoIP	19955415
Intra	DHX9	Q08211	NXF1	Homo sapiens	Q9UBU9	Anti Bait CoIP	10924507
Intra	DHX9	Q08211	EIF2AK2	Homo sapiens	P19525	Anti Tag CoIP	35271311
Intra	DHX9	Q08211	RELA	Homo sapiens	Q04206	CoIP	15355351
Intra	DHX9	Q08211	RELA	Homo sapiens	Q04206	Anti Tag CoIP	15355351
Intra	DHX9	Q08211	RELA	Homo sapiens	Q04206	Pull Down	15355351
Intra	DHX9	Q08211	PRMT1	Homo sapiens	Q99873	Anti Tag CoIP	35271311
Intra	DHX9	Q08211	PRMT1	Homo sapiens	Q99873	Pull Down	15084609
Cross	DHX9	Q08211	P19712-PRO_0000038050	Classical swine fever virus	P19712-PRO_0000038050	Pull Down	24965446
Cross	DHX9	Q08211	P19712-PRO_0000038050	Classical swine fever virus	P19712-PRO_0000038050	IF	24965446

Cross: Cross-species interaction Intra: Intraspecies interaction

DHX9 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P82125	RNA Helicase A Antibody (YA1870)	WB	Rat
HY-P82125A	RNA Helicase A Antibody (YA1870)(PBS only)	WB	Rat

Related Diseases

Diseases	Alias
Werner Syndrome	Werner'S Syndrome WRN Adult Progeria Ws Adult Premature Ageing Syndrome Adult Premature Aging Syndrome Werners Syndrome
Chikungunya	Chikungunya Fever Arbovirus A Chikungunya Type Chik Chikv Infection Ck Chikungunya Virus Infection Chikungunya Haemorrhagic Fever Chikungunya Viral Disease Chikungunya Mosquito-Borne Viral Fever
Parkinson Disease 22, Autosomal Dominant	PARK22 Parkinson'S Disease 22 Autosomal Dominant Parkinson'S Disease 22 Parkinson Disease 22
Abnormal Retinal Correspondence
Avian Influenza	Bird Flu Influenza In Birds Avian Flu

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (23)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-156782	DHX9-IN-1	Inhibitor	98.33%	Unkown
HY-160007	DHX9-IN-3	Inhibitor	98.67%	Unkown
HY-157604	DHX9-IN-9	Inhibitor	99.79%	Unkown
HY-160009	DHX9-IN-5	Inhibitor	99.75%	Unkown
HY-160008	DHX9-IN-4	Inhibitor	98.54%	Unkown
HY-160010	DHX9-IN-6	Inhibitor	98.86%	Unkown
HY-RS03761	DHX9 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-157602	DHX9-IN-8	Inhibitor	/	Unkown
HY-157603	DHX9-IN-7	Inhibitor	/	Unkown
HY-157605	DHX9-IN-11	Inhibitor	/	Unkown
HY-157606	DHX9-IN-13	Inhibitor	/	Unkown
HY-157607	DHX9-IN-15	Inhibitor	/	Unkown
HY-157608	DHX9-IN-10	Inhibitor	/	Unkown
HY-157609	DHX9-IN-12	Inhibitor	/	Unkown
HY-157610	DHX9-IN-14	Inhibitor	/	Unkown
HY-157611	DHX9-IN-17	Inhibitor	/	Unkown
HY-157612	DHX9-IN-16	Inhibitor	/	Unkown
HY-160813	DHX9-IN-18	Inhibitor	/	Unkown
HY-170684	DHX9-IN-19	Inhibitor	/	Unkown
HY-179322	DHX9-IN-21	Inhibitor	/	Unkown
HY-181440	TDG-IN-1	Inhibitor	/	Unkown
HY-RS18237	Dhx9 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS24716	Dhx9 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Mus musculus	DHX9	MGD	MGI:108177
Bos taurus	DHX9	VGNC	VGNC:28060
Felis catus	DHX9	VGNC	VGNC:61488
Rattus norvegicus	DHX9	RGD	RGD:1308548
Macaca mulatta	DHX9	VGNC	VGNC:71766
Canis familiaris	DHX9	VGNC	VGNC:39953
Others	DHX9	NCBI