PRKAA1 - protein kinase AMP-activated catalytic subunit alpha 1 Gene

Also Known as AMPK; AMPKa1; AMPK alpha 1

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 5562

About PRKAA1

Cytogenetic location: 5p13.1 Genomic coordinates (GRCh38): 5:40,759,389-40,798,374 (from NCBI)

This gene has 9 transcripts (splice variants), 229 orthologues and 17 paralogues. Ubiquitous expression in gall bladder (RPKM 18.4), thyroid (RPKM 16.3) and 25 other tissues.

Summary

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic Enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

PRKAA1 Products (8)

mRNA	Protein	Name
NM_001355028.2	NP_001341957.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 3
NM_001355029.2	NP_001341958.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 4
NM_001355034.2	NP_001341963.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 5
NM_001355035.2	NP_001341964.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 6
NM_001355036.2	NP_001341965.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 6
NM_001355037.2	NP_001341966.1	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 6
NM_006251.6	NP_006242.5	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1
NM_206907.4	NP_996790.3	5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform 2

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables AMP-activated protein kinase activity	IDA IDA: Inferred from direct assay	14651849	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	16306228	GOA
enables protein kinase activity	IDA IDA: Inferred from direct assay	17028174	GOA
enables protein serine/threonine kinase activity	IDA IDA: Inferred from direct assay	18439900	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in CAMKK-AMPK signaling cascade	IMP IMP: Inferred from mutant phenotype	26103054	GOA
involved in cellular response to glucose starvation	IDA IDA: Inferred from direct assay	14651849	GOA
involved in cellular response to nutrient levels	IDA IDA: Inferred from direct assay	25412657	GOA
involved in cellular response to stress	IDA IDA: Inferred from direct assay	37079666	GOA
acts upstream of positive effect lipid droplet disassembly	IDA IDA: Inferred from direct assay	34077757	GOA
involved in motor behavior	IGI IGI: Inferred from genetic interaction	21768291	GOA
involved in negative regulation of TORC1 signaling	IDA IDA: Inferred from direct assay	14651849	GOA
involved in negative regulation of hepatocyte apoptotic process	IDA IDA: Inferred from direct assay	32029622	GOA
involved in neuron cellular homeostasis	IGI IGI: Inferred from genetic interaction	21768291	GOA
involved in positive regulation of T cell activation	IDA IDA: Inferred from direct assay	38377992	GOA
involved in positive regulation of T cell mediated immune response to tumor cell	IDA IDA: Inferred from direct assay	38377992	GOA
involved in positive regulation of autophagy	IDA IDA: Inferred from direct assay	22012985	GOA
involved in positive regulation of autophagy	IMP IMP: Inferred from mutant phenotype	26103054	GOA
involved in positive regulation of gene expression	IDA IDA: Inferred from direct assay	17028174	GOA
involved in protein localization to lipid droplet	IDA IDA: Inferred from direct assay	34077757	GOA
involved in protein phosphorylation	IDA IDA: Inferred from direct assay	17028174	GOA

Cellular Component GO Annotation	Evidence	References	Source
is active in cytoplasm	IDA IDA: Inferred from direct assay	18381428	GOA
located in cytoplasm	IDA IDA: Inferred from direct assay	21768291	GOA
part of nucleotide-activated protein kinase complex	IPI IPI: Inferred from physical interaction	25412657	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

PRKAA1 Protein Structure

Pkinase

0
100
200
300
400
500
559 a.a.

Protein Preferred Names	Protein Names
5'-AMP-activated protein kinase catalytic subunit alpha-1	5'-AMP-activated protein kinase, catalytic alpha-1 chain

PRKAA1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	PRKAA1	Q13131	PNMA5	Homo sapiens	Q96PV4	Y2H Array	25416956
Intra	PRKAA1	Q13131	PNMA5	Homo sapiens	Q96PV4	Y2H Array	31515488
Intra	PRKAA1	Q13131	KRT40	Homo sapiens	Q6A162	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	KRT40	Homo sapiens	Q6A162	Validated Y2H	25416956
Intra	PRKAA1	Q13131	KRT40	Homo sapiens	Q6A162	Y2H Array	25416956
Intra	PRKAA1	Q13131	CDX4	Homo sapiens	O14627	Validated Y2H	25416956
Intra	PRKAA1	Q13131	CDX4	Homo sapiens	O14627	Y2H Array	25416956
Intra	PRKAA1	Q13131	CDX4	Homo sapiens	O14627	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	RIMBP3	Homo sapiens	Q9UFD9	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	RIMBP3	Homo sapiens	Q9UFD9	Y2H Array	25416956
Intra	PRKAA1	Q13131	PRKAB2	Homo sapiens	O43741	Y2H Array	25416956
Intra	PRKAA1	Q13131	PRKAB2	Homo sapiens	O43741	Anti Tag CoIP	20562859
Intra	PRKAA1	Q13131	PRKAB2	Homo sapiens	O43741	TAP	16306228
Intra	PRKAA1	Q13131	PRKAB2	Homo sapiens	O43741	Anti Tag CoIP	25852190
Intra	PRKAA1	Q13131	PRKAB2	Homo sapiens	O43741	TAP	23455922
Intra	PRKAA1	Q13131	PRKAB2	Homo sapiens	O43741	Anti Bait CoIP	22363791
Intra	PRKAA1	Q13131	PRKAB2	Homo sapiens	O43741	Y2H Array	21988832
Intra	PRKAA1	Q13131	PRKAB2	Homo sapiens	O43741	Pull Down	32707033
Intra	PRKAA1	Q13131	PRKAB2	Homo sapiens	O43741	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	PRKAB2	Homo sapiens	O43741	Anti Tag CoIP	35271311
Intra	PRKAA1	Q13131	FSBP	Homo sapiens	O95073	Y2H Array	25416956
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	TAP	23455922
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	Y2H Array	25416956
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	TAP	16306228
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	Anti Tag CoIP	25852190
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	Anti Tag CoIP	33961781
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	Anti Bait CoIP	22363791
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	Pull Down	32707033
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	Anti Tag CoIP	35271311
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	Y2H Array	31515488
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	Anti Tag CoIP	20562859
Intra	PRKAA1	Q13131	PRKAG1	Homo sapiens	P54619	Validated Y2H	25416956
Intra	PRKAA1	Q13131	ROPN1	Homo sapiens	Q9HAT0	Y2H Array	31515488
Intra	PRKAA1	Q13131	ROPN1	Homo sapiens	Q9HAT0	Validated Y2H	25416956
Intra	PRKAA1	Q13131	SSX2IP	Homo sapiens	Q9Y2D8	Y2H Array	25416956
Intra	PRKAA1	Q13131	SSX2IP	Homo sapiens	Q9Y2D8	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	SSX2IP	Homo sapiens	Q9Y2D8	Validated Y2H	25416956
Intra	PRKAA1	Q13131	HMBOX1	Homo sapiens	Q6NT76	Validated Y2H	25416956
Intra	PRKAA1	Q13131	HMBOX1	Homo sapiens	Q6NT76	Y2H Array	25416956
Intra	PRKAA1	Q13131	L3MBTL3	Homo sapiens	Q96JM7	Validated Y2H	25416956
Intra	PRKAA1	Q13131	L3MBTL3	Homo sapiens	Q96JM7	Y2H Array	25416956
Intra	PRKAA1	Q13131	VPS52	Homo sapiens	Q8N1B4	Validated Y2H	25416956
Intra	PRKAA1	Q13131	HSP90AB1	Homo sapiens	P08238	TAP	23455922
Intra	PRKAA1	Q13131	VPS37B	Homo sapiens	Q9H9H4	Y2H Array	25416956
Intra	PRKAA1	Q13131	VPS37B	Homo sapiens	Q9H9H4	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	GOLGA2	Homo sapiens	Q08379	Validated Y2H	25416956
Intra	PRKAA1	Q13131	GOLGA2	Homo sapiens	Q08379	Y2H Array	25416956
Intra	PRKAA1	Q13131	BHLHE40	Homo sapiens	O14503	Validated Y2H	25416956
Intra	PRKAA1	Q13131	BHLHE40	Homo sapiens	O14503	Y2H Array	25416956
Intra	PRKAA1	Q13131	TLE5	Homo sapiens	Q08117	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	TLE5	Homo sapiens	Q08117	Y2H Array	25416956
Intra	PRKAA1	Q13131	TRIM27	Homo sapiens	P14373	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	TRIM27	Homo sapiens	P14373	Validated Y2H	25416956
Intra	PRKAA1	Q13131	PRKAB1	Homo sapiens	Q9Y478	TAP	23455922
Intra	PRKAA1	Q13131	PRKAB1	Homo sapiens	Q9Y478	Anti Tag CoIP	18480843
Intra	PRKAA1	Q13131	PRKAB1	Homo sapiens	Q9Y478	TAP	16306228
Intra	PRKAA1	Q13131	PRKAB1	Homo sapiens	Q9Y478	Anti Bait CoIP	22363791
Intra	PRKAA1	Q13131	PRKAB1	Homo sapiens	Q9Y478	Pull Down	32707033
Intra	PRKAA1	Q13131	PRKAB1	Homo sapiens	Q9Y478	Anti Tag CoIP	35271311
Intra	PRKAA1	Q13131	PRKAB1	Homo sapiens	Q9Y478	Anti Tag CoIP	20562859
Intra	PRKAA1	Q13131	PRKAB1	Homo sapiens	Q9Y478	Anti Tag CoIP	33961781
Intra	PRKAA1	Q13131	PRKAB1	Homo sapiens	Q9Y478	Anti Tag CoIP	25852190
Intra	PRKAA1	Q13131	TSC22D4	Homo sapiens	Q9Y3Q8	Validated Y2H	25416956
Intra	PRKAA1	Q13131	TSC22D4	Homo sapiens	Q9Y3Q8	Y2H Array	25416956
Intra	PRKAA1	Q13131	ZBED1	Homo sapiens	O96006	Y2H Array	25416956
Intra	PRKAA1	Q13131	ZBED1	Homo sapiens	O96006	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	TRIP6	Homo sapiens	Q15654	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	TRIP6	Homo sapiens	Q15654	Validated Y2H	25416956
Intra	PRKAA1	Q13131	MTUS2	Homo sapiens	Q5JR59	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	ABI2	Homo sapiens	Q9NYB9	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	ABI2	Homo sapiens	Q9NYB9	Validated Y2H	25416956
Intra	PRKAA1	Q13131	ABI2	Homo sapiens	Q9NYB9	Y2H Array	25416956
Intra	PRKAA1	Q13131	ABI2	Homo sapiens	Q9NYB9	Y2H Array	29892012
Intra	PRKAA1	Q13131	UBXN11	Homo sapiens	Q5T124	Validated Y2H	25416956
Intra	PRKAA1	Q13131	RFX6	Homo sapiens	Q8HWS3	Y2H Prey Pooling	25416956
Intra	PRKAA1	Q13131	IKZF3	Homo sapiens	Q9UKT9	Validated Y2H	25416956
Intra	PRKAA1	Q13131	INO80E	Homo sapiens	Q8NBZ0	Y2H Prey Pooling	25416956

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant PRKAA1 Proteins

Cat. No.	Product Name	Accession	Purity
HY-P76141	AMPK gamma 1/beta 1/alpha 1 Heterotrimer Protein, Human (Active, sf9, His-GST)	P54619-1 (M1-P331)&Q9Y478 (M1-I270)&Q13131-1 (M1-Q559)	≥ 90%, as determined by reducing SDS-PAGE.
HY-P76143	AMPK gamma 1/beta 2/alpha 1 Heterotrimer Protein, Human (Active, sf9, His-GST)	P54619-1 (M1-P331)&O43741 (M1-I272)&Q13131-1 (M1-Q559)	≥ 90%, as determined by reducing SDS-PAGE.

PRKAA1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80376	AMPK alpha 1 Antibody (YA624)	WB, ICC/IF, FC, IP	Human, Rat
HY-P80451	Phospho-AMPK alpha 1 (Ser496) Antibody (YA226)	WB, ICC/IF, IP	Human
HY-P80539	AMPK alpha 1 Antibody	WB, IHC-P, ICC/IF, IP, FC	Human, Mouse, Rat
HY-P80790	Phospho-AMPK alpha 1 (Ser496) Antibody	WB, IHC-P, ICC/IF	Human
HY-P84528	AMPK alpha 1 Antibody (YA4225)	WB, IHC-P, ICC/IF, FC, ELISA, ELISA	Human, Mouse, Rat, Monkey
HY-P84528A	AMPK alpha 1 Antibody (YA4225)(PBS only)	WB, IHC-P, ICC/IF, FC, ELISA, ELISA	Human, Mouse, Rat, Monkey
HY-P85745	AMPK alpha 1 Antibody (YA5437)	WB, IHC-P, ICC/IF	Human

Related Diseases

Diseases	Alias
Wolff-Parkinson-White Syndrome	Wolff-Parkinson-White Pattern Wpw Syndrome Anomalous Atrioventricular Excitation Anomalous A-V Excitation Ventricular Pre-Excitation With Arrhythmia WPWS Ventricular Familial Preexcitation Syndrome Preexcitation Syndrome Ventricular Preexcitation Wpw - [Wolff-Parkinson- White] Syndrome Pre-Excitation Syndrome
Myotonia
Cardia Cancer	Ca Cardia - Stomach Malignant Neoplasm Of Cardia Of Stomach
Neurodegeneration With Brain Iron Accumulation	Nbia Neurodegeneration With Brain Iron Accumulation Disorders Neurodegeneration, With Brain Iron Accumulation
Lung Cancer	Lung Carcinoma Non-Small Cell Lung Carcinoma Lung Cancer, Susceptibility To Lung Cancer, Protection Against Adenocarcinoma Of Lung, Somatic Adenocarcinoma Of Lung, Response To Tyrosine Kinase Inhibitor In Nonsmall Cell Lung Cancer Lung Neoplasm Carcinoma Of Lung Lung Non-Small Cell Carcinoma Non-Small Cell Lung Cancer Nsclc Lung Neoplasms Malignant Neoplasm Of Lung Alveolar Cell Carcinoma Nonsmall Cell Lung Cancer, Somatic Nonsmall Cell Lung Cancer, Response To Tyrosine Kinase Inhibitor In Nonsmall Cell Lung Cancer, Susceptibility To Lung Cancer, Somatic Lung Cancer, Resistance To Cancer Of Lung Cancer Of Bronchus Cancer Of The Lung Lung Malignancies Lung Malignant Tumors Malignant Lung Tumor Malignant Tumor Of Lung Pulmonary Cancer Pulmonary Carcinoma Pulmonary Neoplasms Respiratory Carcinoma LNCR Adenocarcinoma Of Lung Neoplasm Of Lung Cancer Lung Carcinoma Non-Small Cell Lung Carcinoma, Non-Small-Cell Lung Lung Cancers Lung Carcinomas Cancer, Lung Cancer, Lung, Non-Small Cell Primary Malignant Neoplasm Of Lung Bronchioloalveolar Adenocarcinoma
Body Mass Index Quantitative Trait Locus 11	OBESITY Obesity, Susceptibility To Leanness, Inherited Obesity, Susceptibility To, Bmiq11 Obesity, Mild, Early-Onset Obesity, Association With Obesity, Early-Onset, Susceptibility To Obesity, Severe Obesity, Severe, And Type Ii Diabetes Obesity, Late-Onset Obesity , Susceptibility To BMIQ11 Obesity Bmiq11 Obesity, Early-Onset Simple Obesity Nos Excess Fat Obesity, Not Elsewhere Classified, Body Mass Index Not Elsewhere Classified Adiposis
Type 2 Diabetes Mellitus	Insulin Resistance NIDDM Type 2 Diabetes Diabetes Mellitus, Non-Insulin-Dependent T2D Noninsulin-Dependent Diabetes Mellitus Diabetes Mellitus, Type Ii Maturity-Onset Diabetes Insulin Resistance, Severe, Digenic Diabetes Mellitus, Type 2 Diabetes Mellitus, Noninsulin-Dependent Diabetes Mellitus, Noninsulin-Dependent, Association With Diabetes Mellitus, Noninsulin-Dependent, Late Onset Hypertension, Insulin Resistance-Related, Susceptibility To Insulin Resistance, Susceptibility To Non-Insulin-Dependent Diabetes Mellitus Type Ii Diabetes Mellitus Adult-Onset Diabetes Mellitus Maturity-Onset Diabetes Mellitus Diabetes Mellitus Type 2 Type Ii Diabetes Type 2 Diabetes Mellitus, Susceptibility To Diabetes, Type 2 Diabetes Mellitus, Noninsulin-Dependent, Susceptibility To Diabetes Mellitus, Non-Insulin-Dependent, Susceptibility To Diabetes Mellitus, Type 2, Susceptibility To Diabetes Mellitus, Noninsulin-Dependent, 2 Diabetes Mellitus, Type Ii, Susceptibility To Hypertension, Insulin Resistance-Related Adult-Onset Diabetes Aodm Diabetes Mellitus, Adult-Onset Diabetes Mellitus Type Ii Diabetes Mellitus Type 2, Susceptibility To Diabetes, Type Ii, Susceptibility To Diabetes Type 2 Diabetes Mellitus Adult Onset Diabetes Maturity Onset Diabetes Nonketotic Diabetes Non-Insulin Dependent Diabetes Mellitus T2dm - [Type 2 Diabetes Mellitus] Niddm - [Non Insulin Dependent Diabetes Mellitus] Dm2 Dm Type Ii Diabetic Type 2 Insulin Requiring Type 2 Diabetes Noninsulin Dependent Diabetes Non-Insulin-Dependent Diabetes Mellitus Without Complications Diabetes Due To Insulin Secretory Defect Diabetes Mellitus Due To Insulin Secretory Defect Non-Insulin-Dependent Diabetes Of The Young Senile Diabetes Nonketotic Hyperglycaemia Stable Diabetes
Diabetes Mellitus	Diabetes

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (7)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-148065	FMF-06-098-1	Inhibitor	98.77%	Unkown
HY-RS11100	PRKAA1 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-P11779	Ac-RKAA-PABC-MMAE	Inhibitor	/	Unkown
HY-P11779A	Ac-RKAA-PABC-MMAE TFA	Inhibitor	/	Unkown
HY-RS29496	Prkaa1 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-117356A	MRS2693 ammonium		/	Unkown
HY-185634	PROTAC GSK3 degrader-1		/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Mus musculus	PRKAA1	MGD	MGI:2145955
Macaca mulatta	PRKAA1	VGNC	VGNC:76399
Rattus norvegicus	PRKAA1	RGD	RGD:3387
Felis catus	PRKAA1	VGNC	VGNC:69045
Bos taurus	PRKAA1	VGNC	VGNC:50248
Canis familiaris	PRKAA1	VGNC	VGNC:51914
Others	PRKAA1	NCBI