RBX1 - ring-box 1 Gene

Also Known as ROC1; RNF75; BA554C12.1

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 9978

About RBX1

Cytogenetic location: 22q13.2 Genomic coordinates (GRCh38): 22:40,951,378-40,973,309 (from NCBI)

This gene has 3 transcripts (splice variants), 223 orthologues and 2 paralogues. Ubiquitous expression in placenta (RPKM 27.7), bone marrow (RPKM 27.1) and 25 other tissues.

Summary

This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.[provided by RefSeq, Sep 2010]

RBX1 Products (1)

mRNA	Protein	Name
NM_014248.4	NP_055063.1	E3 ubiquitin-protein ligase RBX1

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables NEDD8 ligase activity	IDA IDA: Inferred from direct assay	19250909	GOA
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI IPI: Inferred from physical interaction	14739464	GOA
enables cullin family protein binding	IDA IDA: Inferred from direct assay	10230407	GOA
enables cullin family protein binding	IPI IPI: Inferred from physical interaction	14528312	GOA
enables molecular adaptor activity	EXP EXP: Inferred from Experiment	11961546	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	11961546	GOA
enables ubiquitin protein ligase activity	IDA IDA: Inferred from direct assay	10230407	GOA
enables ubiquitin protein ligase binding	IDA IDA: Inferred from direct assay	17636018	GOA
contributes to ubiquitin-protein transferase activity	IDA IDA: Inferred from direct assay	15983046	GOA
enables ubiquitin-ubiquitin ligase activity	IDA IDA: Inferred from direct assay	10230407	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in DNA damage response	EXP EXP: Inferred from Experiment	22118460	GOA
involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IDA IDA: Inferred from direct assay	15103331	GOA
involved in T cell activation	IDA IDA: Inferred from direct assay	34065512	GOA
involved in cellular response to UV	EXP EXP: Inferred from Experiment	22118460	GOA
involved in cellular response to amino acid stimulus	IDA IDA: Inferred from direct assay	29769719	GOA
involved in negative regulation of response to oxidative stress	IDA IDA: Inferred from direct assay	11027288	GOA
involved in negative regulation of type I interferon production	IDA IDA: Inferred from direct assay	36394357	GOA
involved in positive regulation of TORC1 signaling	IDA IDA: Inferred from direct assay	29769719	GOA
involved in positive regulation of canonical NF-kappaB signal transduction	IDA IDA: Inferred from direct assay	10644755	GOA
involved in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IPI IPI: Inferred from physical interaction	14739464	GOA
involved in positive regulation of protein autoubiquitination	IMP IMP: Inferred from mutant phenotype	14528312	GOA
involved in positive regulation of protein catabolic process	IDA IDA: Inferred from direct assay	11027288	GOA
involved in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA IDA: Inferred from direct assay	10230407	GOA
involved in protein K48-linked ubiquitination	IDA IDA: Inferred from direct assay	15103331	GOA
involved in protein monoubiquitination	IDA IDA: Inferred from direct assay	22358839	GOA
involved in protein neddylation	IDA IDA: Inferred from direct assay	19250909	GOA
involved in protein polyubiquitination	IDA IDA: Inferred from direct assay	14528312	GOA
involved in protein ubiquitination	IDA IDA: Inferred from direct assay	15103331	GOA
involved in protein ubiquitination	IPI IPI: Inferred from physical interaction	14739464	GOA
involved in spermatogenesis	IDA IDA: Inferred from direct assay	34065512	GOA
involved in ubiquitin-dependent protein catabolic process	IDA IDA: Inferred from direct assay	17636018	GOA
involved in ubiquitin-dependent protein catabolic process via the C-end degron rule pathway	IDA IDA: Inferred from direct assay	29779948	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of Cul2-RING ubiquitin ligase complex	IDA IDA: Inferred from direct assay	17636018	GOA
part of Cul3-RING ubiquitin ligase complex	IDA IDA: Inferred from direct assay	14528312	GOA
part of Cul4A-RING E3 ubiquitin ligase complex	EXP EXP: Inferred from Experiment	22118460	GOA
part of Cul4A-RING E3 ubiquitin ligase complex	IDA IDA: Inferred from direct assay	12732143	GOA
part of Cul4B-RING E3 ubiquitin ligase complex	IDA IDA: Inferred from direct assay	18794347	GOA
part of Cul5-RING ubiquitin ligase complex	IDA IDA: Inferred from direct assay	17636018	GOA
part of Cul7-RING ubiquitin ligase complex	IDA IDA: Inferred from direct assay	18498745	GOA
part of SCF ubiquitin ligase complex	IDA IDA: Inferred from direct assay	11956208	GOA
part of cullin-RING ubiquitin ligase complex	IDA IDA: Inferred from direct assay	22405651	GOA
is active in cytoplasm	IDA IDA: Inferred from direct assay	34065512	GOA
is active in nucleus	IDA IDA: Inferred from direct assay	11027288	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

RBX1 Protein Structure

zf-rbx1

0
100
108 a.a.

Protein Preferred Names	Protein Names
E3 ubiquitin-protein ligase RBX1	E3 ubiquitin-protein transferase RBX1

RBX1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	RBX1	P62877	KRTAP12-2	Homo sapiens	P59991	Y2H Prey Pooling	32296183
Intra	RBX1	P62877	KRTAP12-2	Homo sapiens	P59991	Y2H Array	32296183
Intra	RBX1	P62877	PBX4	Homo sapiens	Q9BYU1	Validated Y2H	32296183
Intra	RBX1	P62877	PBX4	Homo sapiens	Q9BYU1	Y2H Prey Pooling	32296183
Intra	RBX1	P62877	PBX4	Homo sapiens	Q9BYU1	Y2H Array	32296183
Intra	RBX1	P62877	UBE2M	Homo sapiens	P61081	NMR	21765416
Intra	RBX1	P62877	UBE2M	Homo sapiens	P61081	Ubiquitinase Assay	24949976
Intra	RBX1	P62877	UBE2M	Homo sapiens	P61081	Y2H Prey Pooling	32296183
Intra	RBX1	P62877	UBE2M	Homo sapiens	P61081	Y2H Array	32296183
Intra	RBX1	P62877	UBE2M	Homo sapiens	P61081	Neddylase Assay	24949976
Intra	RBX1	P62877	CUL1	Homo sapiens	Q13616	Ubiquitinase Assay	24949976
Intra	RBX1	P62877	CUL1	Homo sapiens	Q13616	SAXS	18805092
Intra	RBX1	P62877	CUL1	Homo sapiens	Q13616	Neddylase Assay	24949976
Intra	RBX1	P62877	CUL1	Homo sapiens	Q13616	Anti Tag CoIP	33692209
Intra	RBX1	P62877	CUL1	Homo sapiens	Q13616	Anti Bait CoIP	12504026
Intra	RBX1	P62877	CUL1	Homo sapiens	Q13616	Anti Tag CoIP	33961781
Intra	RBX1	P62877	CUL4B	Homo sapiens	Q13620	Anti Tag CoIP	23238014
Intra	RBX1	P62877	CUL4B	Homo sapiens	Q13620	Anti Tag CoIP	33961781
Intra	RBX1	P62877	CUL2	Homo sapiens	Q13617	Anti Tag CoIP	33961781
Intra	RBX1	P62877	CUL2	Homo sapiens	Q13617	Validated Y2H	32296183
Intra	RBX1	P62877	CUL2	Homo sapiens	Q13617	Y2H Array	32296183
Intra	RBX1	P62877	CUL2	Homo sapiens	Q13617	Y2H Prey Pooling	32296183
Intra	RBX1	P62877	CUL2	Homo sapiens	Q13617	Pull Down	17183367
Intra	RBX1	P62877	SKP2	Homo sapiens	Q13309	Anti Tag CoIP	33961781
Intra	RBX1	P62877	SKP2	Homo sapiens	Q13309	Anti Tag CoIP	18239684
Intra	RBX1	P62877	GLMN	Homo sapiens	Q92990	Anti Tag CoIP	33961781
Intra	RBX1	P62877	GLMN	Homo sapiens	Q92990	Validated Y2H	32296183
Intra	RBX1	P62877	GLMN	Homo sapiens	Q92990	Y2H Array	32296183
Intra	RBX1	P62877	GLMN	Homo sapiens	Q92990	Y2H Prey Pooling	32296183

Cross: Cross-species interaction Intra: Intraspecies interaction

RBX1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P81379	RBX1 Antibody (YA1124)	IHC-P	Human
HY-P81379A	RBX1 Antibody (YA1124)(PBS only)	IHC-P	Human

Related Diseases

Diseases	Alias
Invasive Bladder Transitional Cell Carcinoma	Invasive Bladder Urothelial Carcinoma Infiltrating Bladder Urothelial Carcinoma
Mulibrey Nanism	MUL Muscle-Liver-Brain-Eye Nanism Pericardial Constriction And Growth Failure Perheentupa Syndrome Mulibrey Growth Disorder Mulibrey Nanism Syndrome Pericardial Constriction With Growth Failure Nanism Mulibrey
Smith-Magenis Syndrome	SMS Chromosome 17p11.2 Deletion Syndrome 17p11.2 Microdeletion Syndrome 17p11.2 Monosomy Chromosome 17p Deletion Syndrome Del(17) P11.2 17p- Syndrome Deletion 17p Syndrome Partial Monosomy 17p
Cockayne Syndrome	Cockayne'S Syndrome Dwarfism-Retinal Atrophy-Deafness Syndrome Neill-Dingwall Syndrome Progeria-Like Syndrome Progeroid Nanism Cs
Xeroderma Pigmentosum, Complementation Group E	Xeroderma Pigmentosum V Xp5 Xeroderma Pigmentosum, Group E, Ddb-Negative Subtype Xpe Xp, Group E Xeroderma Pigmentosum, Type 5 Xeroderma Pigmentosum Complementation Group E XP-E Xp Group E
Three M Syndrome 1	3-M Syndrome Yakut Short Stature Syndrome 3m Syndrome Le Merrer Syndrome Dolichospondylic Dysplasia Gloomy Face Syndrome Three M Syndrome 3M1 3m Syndrome 1 Miller-Mckusick-Malvaux Syndrome 3-Msbn Three-M Slender-Boned Nanism Miller-Mckusick-Malvaux-Syndrome 3-M Syndrome 1 3m Syndrome-1 3m Syndrome, Type 1 Dwarfism Dwarfism Tall Vertebrae
Retinal Hemangioblastoma	Capillary Hemangioma Of Retina
Kidney Cancer	Renal Cancer Renal Carcinoma Kidney Neoplasm Malignant Neoplasm Of Kidney Except Pelvis Malignant Tumour Of Kidney Kidney Neoplasms Cancer, Kidney Cancer, Renal Malignant Neoplasm Of Kidney Renal Cell Carcinoma
Renal Cell Carcinoma, Nonpapillary	Renal Cell Carcinoma RCC Nonpapillary Renal Cell Carcinoma Clear Cell Renal Cell Carcinoma Hypernephroma Adenocarcinoma Of Kidney Renal Carcinoma, Chromophobe, Somatic Clear Cell Carcinoma Of Kidney Clear-Cell Metastatic Renal Cell Carcinoma Clear Cell Renal Carcinoma Renal Cell Carcinoma, Somatic Conventional Renal Cell Carcinoma Conventional Renal Cell Carcinoma Renal Clear Cell Carcinoma Ccrcc Hereditary Clear Cell Renal Cell Carcinoma Carcinoma, Renal Cell Renal Cell Carcinoma, Clear Cell, Somatic Renal Cell Carcinoma, Clear Cell Clear Cell Kidney Carcinoma Clear Cell Rcc Cystic-Multilocular Variant Clear Cell Renal Cell Adenocarcinoma Hereditary Clear Cell Renal Cell Adenocarcinoma Common Renal Cell Carcinoma Crcc Renal Cell Carcinoma Non-Papillary Carcinoma Renal Cell Renal Cell Cancer Carcinoma, Renal Cell, Nonpapillary
Breast Cancer	Breast Carcinoma Male Breast Cancer Breast Cancer, Familial Malignant Neoplasm Of Breast Breast Cancer, Susceptibility To Breast Cancer, Early-Onset Malignant Tumor Of Breast Carcinoma Of Male Breast Breast Cancer, Invasive Ductal Breast Cancer, Protection Against Breast Cancer, Somatic Breast Cancer, Male Breast Cancer, Lobular, Somatic Breast Tumor Mammary Cancer Mammary Tumor Malignant Neoplasm Of Male Breast Mammary Carcinoma Male Breast Carcinoma Familial Cancer Of Breast Invasive Ductal Breast Carcinoma Breast Cancer Susceptibility Breast Cancer, Male, Susceptibility To Breast Cancer, Early-Onset, Susceptibility To Malignant Tumor Of The Breast Mammary Neoplasm Primary Breast Cancer Neoplasm Of Male Breast Carcinoma Of Breast Breast Cancer In Men Familial Breast Cancer Cancer Of Breast BC Breast Cancer Familial Breast Cancer Familial Male Breast Cancer, Familial Male Breast Male Carcinoma Breast Neoplasms Breast Neoplasms, Male Mammary Tumors Mammary Carcinomas Cancer, Breast Cancer, Breast, Susceptibility Invasive Breast Ductal Carcinoma Breast Neoplasm Susceptibility To Breast Cancer Mammary Neoplasms Animal Mammary Neoplasms Primary Malignant Neoplasm Of Breast Infiltrating Ductal Carcinoma Of Breast Infiltrating Duct Carcinoma Of Unspecified Site Infiltrating Ductular Carcinoma Of Unspecified Site Invasive Breast Carcinoma Of No Special Type Microinvasive Carcinoma Of Breast Carcinoma With Apocrine Differentiation
Uv-Sensitive Syndrome	Uvss Uv Sensitive Syndrome Ultraviolet Sensitive Syndrome
Erythrocytosis, Familial, 2	Chuvash Polycythemia ECYT2 Familial Erythrocytosis 2 Autosomal Recessive Benign Erythrocytosis Polycythemia, Vhl-Dependent Chuvash Erythromatosis Chuvash Type Polycythemia Chuvash Erythrocytosis Von Hippel-Lindau-Dependent Polycythemia Polycythemia Chuvash Type Vhl-Dependent Polycythemia Erythrocytosis, Familial, Type 2
Cockayne Syndrome A	Cockayne Syndrome Type 1 Cockayne Syndrome, Type A Cockayne Syndrome Type I CSA Cockayne Syndrome Classic Form Cockayne Syndrome Classical Cockayne Syndrome Type A Ckn1
Autosomal Recessive Intellectual Developmental Disorder	Mental Retardation, Autosomal Recessive Autosomal Recessive Mental Retardation Autosomal Recessive Non-Syndromic Mental Retardation Autosomal Recessive Non-Syndromic Intellectual Disability
Von Hippel-Lindau Syndrome	Von Hippel-Lindau Disease Vhl Vhl Syndrome VHLS Von Hippel-Lindau Syndrome, Modifier Of Hippel Lindau Syndrome Angiomatosis Retinae Cerebelloretinal Angiomatosis, Familial Hippel-Lindau Disease Familial Cerebelloretinal Angiomatosis Lindau Disease VHLD
Retinitis Pigmentosa	RP Rod-Cone Dystrophy Autosomal Recessive Retinitis Pigmentosa Non-Syndromic Retinitis Pigmentosa Pericentral Pigmentary Retinopathy Pigmentary Retinopathy Tapetoretinal Degeneration Rcd Retinitis Pigmentosa Autosomal Recessive ARRP Retinitis Pigmentosa, Autosomal Recessive Retinitis Pigmentosa 1

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Approval
Pre-clinical Phase

Approval

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-14658	Thalidomide	Inducer	99.98%	Yes

Pre-clinical Phase

Bioactive Molecules (7)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-14571	E7820	Inhibitor	99.26%	Unkown
HY-RS11776	RBX1 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-14658S	Thalidomide-d4	Inducer	98.71%	Unkown
HY-162240	SJH1-51B	Degrader	/	Unkown
HY-182063	Z226407860	Inhibitor	/	Unkown
HY-RS18150	Rbx1 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS24625	Rbx1 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Rattus norvegicus	RBX1	RGD	RGD:1308453
Canis familiaris	RBX1	VGNC	VGNC:108215
Felis catus	RBX1	VGNC	VGNC:69274
Mus musculus	RBX1	MGD	MGI:1891829
Macaca mulatta	RBX1	VGNC	VGNC:98435
Others	RBX1	NCBI