GTP-γ-S  (Synonyms: Guanosine 5'-[γ-thio]triphosphate)

GTPγS (Guanosine 5'-[γ-thio]triphosphate) is a G-protein activator that protects proteins from proteolytic degradation, stimulates GLUT4 translocation in a tyrosine kinase-dependent manner, stimulate phospholipases and induce actin polymerization. GTPγS to couple with G- protein α, to study its effect on kinase activity. GTPγS acts as a component of lysis buffer^[1][2][3][4].

GTPγS (30-100 μM; 1-3 h) maintains Rab27a in a GTP-bound state that supports its indirect association with melanocyte myosin Va (MC MV) and MC MVΔD in B16 F10 melanocyte lysates, whereas GDP reduces this association^[1].
GTPγS (1 mM; 20 min) stimulates glucose transport in electrically permeabilized rat primary adipocytes via a microtubule-independent mechanism^[2].
GTPγS (1 mM; 20 min) induces GLUT4 translocation to the plasma membrane in electrically permeabilized rat primary adipocytes with more residual cytoplasmic GLUT4 signals than insulin^[2].
GTPγS (1 mM; 20 min) does not disrupt the association of tubulin with GLUT4-containing membranes in electrically permeabilized rat primary adipocytes, in contrast to insulin^[2].
GTPγS (10 μM) defines nonspecific [³⁵S]GTPγS binding in coronal sections of rat brain (including dorsal raphe nucleus, median raphe nucleus, and forebrain areas)^[3].
GTPγS (10 μM; 15 min) formed Gαi2GTPγS that enhances recombinant human Src tyrosine kinase activity with an ED₅₀ of 20 nM, while formed GαzGTPγS reduces this stimulatory effect^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

539.25

C₁₀H₁₆N₅O₁₃P₃S

37589-80-3

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• [1]. Julie G Hensler, et al. Differential Regulation of 5-HT1A Receptor-G Protein Interactions in Brain Following Chronic Antidepressant Administration. Neuropsychopharmacology. 2002 May;26(5):565-73.  [Content Brief]

  [2]. Li-Bin Liu, et al. Insulin recruits GLUT4 from distinct compartments via distinct traffic pathways with differential microtubule dependence in rat adipocytes. J Biol Chem. 2003 Aug 8;278(32):30157-69. doi: 10.1074/jbc.M301511200.  [Content Brief]

  [3]. Xufeng Wu, et al. Rab27a is an essential component of melanosome receptor for myosin Va. Mol Biol Cell. 2002 May;13(5):1735-49.  [Content Brief]

  [4]. Pilar Sánchez-Blázquez, et al. Brain-specific Galphaz interacts with Src tyrosine kinase to regulate Mu-opioid receptor-NMDAR signaling pathway. Cell Signal. 2009 Sep;21(9):1444-54.  [Content Brief]