GTPγS tetralithium
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GTPγS (Guanosine 5'-[γ-thio]triphosphate) tetralithium is a G-protein activator that protects proteins from proteolytic degradation, stimulates GLUT4 translocation in a tyrosine kinase-dependent manner, stimulate phospholipases and induce actin polymerization. GTPγS tetralithium to couple with G- protein α, to study its effect on kinase activity. GTPγS tetralithium acts as a component of lysis buffer.
For research use only. We do not sell to patients.
- Purity: 95.1%
- CAS No.: 94825-44-2
- Formula: C10H12Li4N5O13P3S
- Molecular Weight:562.98
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
GTPγS (30-100 μM; 1-3 h) tetralithium maintains Rab27a in a GTP-bound state that supports its indirect association with melanocyte myosin Va (MC MV) and MC MVΔD in B16 F10 melanocyte lysates, whereas GDP reduces this association[1].
GTPγS tetralithium (1 mM; 20 min) stimulates glucose transport in electrically permeabilized rat primary adipocytes via a microtubule-independent mechanism[2].
GTPγS tetralithium (1 mM; 20 min) induces GLUT4 translocation to the plasma membrane in electrically permeabilized rat primary adipocytes with more residual cytoplasmic GLUT4 signals than insulin[2].
GTPγS tetralithium (1 mM; 20 min) does not disrupt the association of tubulin with GLUT4-containing membranes in electrically permeabilized rat primary adipocytes, in contrast to insulin[2].
GTPγS tetralithium (10 μM) defines nonspecific [³⁵S]GTPγS binding in coronal sections of rat brain (including dorsal raphe nucleus, median raphe nucleus, and forebrain areas)[3].
GTPγS tetralithium (10 μM; 15 min) formed Gαi2GTPγS that enhances recombinant human Src tyrosine kinase activity with an ED50 of 20 nM, while formed GαzGTPγS reduces this stimulatory effect[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 94825-44-2
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Appearance Solid
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Molecular Weight 562.98
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Formula C10H12Li4N5O13P3S
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Color White to off-white
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C(NC(N)=NC3=O)=C3N=C2)O[C@@H]1COP(OP(OP(S[Li])(O[Li])=O)(O[Li])=O)(O[Li])=O
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Synonyms
Guanosine 5'-[γ-thio]triphosphate tetralithium
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 50 mg/mL (88.81 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Wu X, et al. Rab27a is an essential component of melanosome receptor for myosin Va. Mol Biol Cell. 2002;13(5):1735-1749. [Content Brief]
[2]. Liu LB, et al. Insulin recruits GLUT4 from distinct compartments via distinct traffic pathways with differential microtubule dependence in rat adipocytes. J Biol Chem. 2003;278(32):30157-30169. [Content Brief]
[3]. Hensler JG, et al. Differential regulation of 5-HT1A receptor-G protein interactions in brain following chronic antidepressant administration. Neuropsychopharmacology. 2002;26(5):565-573. [Content Brief]
[4]. Sánchez-Blázquez P, et al. Brain-specific Galphaz interacts with Src tyrosine kinase to regulate Mu-opioid receptor-NMDAR signaling pathway. Cell Signal. 2009;21(9):1444-1454. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.7763 mL | 8.8813 mL | 17.7626 mL | 44.4066 mL |
| 5 mM | 0.3553 mL | 1.7763 mL | 3.5525 mL | 8.8813 mL | |
| 10 mM | 0.1776 mL | 0.8881 mL | 1.7763 mL | 4.4407 mL | |
| 15 mM | 0.1184 mL | 0.5921 mL | 1.1842 mL | 2.9604 mL | |
| 20 mM | 0.0888 mL | 0.4441 mL | 0.8881 mL | 2.2203 mL | |
| 25 mM | 0.0711 mL | 0.3553 mL | 0.7105 mL | 1.7763 mL | |
| 30 mM | 0.0592 mL | 0.2960 mL | 0.5921 mL | 1.4802 mL | |
| 40 mM | 0.0444 mL | 0.2220 mL | 0.4441 mL | 1.1102 mL | |
| 50 mM | 0.0355 mL | 0.1776 mL | 0.3553 mL | 0.8881 mL | |
| 60 mM | 0.0296 mL | 0.1480 mL | 0.2960 mL | 0.7401 mL | |
| 80 mM | 0.0222 mL | 0.1110 mL | 0.2220 mL | 0.5551 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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