hCAIX-IN-16
hCAIX-IN-16 (Compound 12d) is hCA IX inhibitor, with Ki values of 190.0 and 187.9 nM for hCA IX and hCA XII, respectively. hCAIX-IN-16 can arrest the cell cycle of breast cancer MDA-MB-468 in G0-G1 and S phase and induce apoptosis. hCAIX-IN-16 shows good broad-spectrum anticancer activity and can be used for cancer research.
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- CAS. Nr.: 2849348-38-3
- Formel: C20H20N8O2S
- Molecular Weight:436.49
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDA-MB-468 | IC50 |
1.48 μM
Compound: 85
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Anticancer activity against human MDA-MB-468 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay
Anticancer activity against human MDA-MB-468 cells assessed as cell growth inhibition incubated for 48 hrs by SRB assay
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[PMID: 39018924] |
In Vitro
hCAIX-IN-16 (3.99 μM; 24 h) arrests cell growth in G0-G1 and S phases in MDA-MB-468 cells[1].
hCAIX-IN-16 (10 µM) inhibits the growth of 44 cancer cell lines with GI% ≥ 10% and shows GI% ≥ 50% against leukemia, (CCRF-CEM, RPMI-8226 and SR) and breast cancer cell lines (T-47D and MDA-MB-468)[1].
hCAIX-IN-16 induces cell death by the apoptotic mechanism in MDA‑MB‑468 cells[1].
Inhibits (under hypoxic conditions) MDA-MB-468 and CCRF-CM cells with IC50s of 3.99 and 4.51 µM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDA-MB-468
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Concentration:3.99 μM
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Incubation Time:24 h
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Result:Induced an increase in cells in G0-G1 and S phases with concurrent significant decrease in G2/M phase.
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Apoptosis Solutions
- Protocol for Cell Cycle
- Breast Cancer Modeling
Chemical Information
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CAS. Nr. 2849348-38-3
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Molecular Weight 436.49
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Formel C20H20N8O2S
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SMILES
O=S(C1=CC=C(NC2=NC(N)=NC(/C(C#N)=C/C3=CC=C(N(C)C)C=C3)=N2)C=C1)(N)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
hCAIX-IN-16 Related Classifications
HY-151630 Related Classifications
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