HDAC3-IN-1

Cat. No.: HY-117374 Purity: 99.28%: Data Sheet
COA

SDS
Handling Instructions
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Technical Support

HDAC3-IN-1 (compound 5) is a potent and selective HDAC3 inhibitor, with an IC₅₀ of 5.96 nM.

For research use only. We do not sell to patients.

HDAC3-IN-1 Chemical Structure

CAS No. : 2044701-99-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE HDAC3-IN-1

•Metab Eng. 2023 Nov:80:94-106. [Abstract]

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View All Isoforms

HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

Biological Activity
Purity & Documentation
References
Customer Review

Description

HDAC3-IN-1 (compound 5) is a potent and selective HDAC3 inhibitor, with an IC₅₀ of 5.96 nM^[1].

IC₅₀ & Target

HDAC3

5.96 ± 0.0 nM (IC₅₀)

HDAC1

36.4 ± 0.2 nM (IC₅₀)

HDAC2

89.9 ± 0.7 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	EC₅₀	> 10000 nM Compound: 3b	Cytotoxicity against human HL-60 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay Cytotoxicity against human HL-60 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay	[PMID: 27754681]
HL-60	GI₅₀	121 nM Compound: 18	Antiproliferative activity against p53-null human HL-60 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis Antiproliferative activity against p53-null human HL-60 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis	[PMID: 38224650]
HL-60	IC₅₀	51.68 nM Compound: 17	Antiproliferative activity against human HL-60 cells assessed as reduction in cell viability after 48 hrs by multiplate reader method Antiproliferative activity against human HL-60 cells assessed as reduction in cell viability after 48 hrs by multiplate reader method	[PMID: 34942071]
K562	EC₅₀	2160 nM Compound: 3b	Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay	[PMID: 27754681]
Kasumi 1	GI₅₀	17.8 nM Compound: 18	Antiproliferative activity against human Kasumi 1 cells harboring wild type p53 assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis Antiproliferative activity against human Kasumi 1 cells harboring wild type p53 assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis	[PMID: 38224650]
MOLM-13	IC₅₀	102.7 nM Compound: 17	Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability measured after 48 hrs by multiplate reader method Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability measured after 48 hrs by multiplate reader method	[PMID: 34942071]
MOLM-14	EC₅₀	76.64 nM Compound: 3b	Cytotoxicity against human MOLM-14 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay Cytotoxicity against human MOLM-14 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay	[PMID: 27754681]
MV4-11	EC₅₀	36.37 nM Compound: 3b	Cytotoxicity against human MV4-11 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay Cytotoxicity against human MV4-11 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay	[PMID: 27754681]
MV4-11	GI₅₀	16 nM Compound: 18	Antiproliferative activity against human MV4-11 cells harboring wild type p53 assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis Antiproliferative activity against human MV4-11 cells harboring wild type p53 assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis	[PMID: 38224650]
MV4-11	IC₅₀	36.37 nM Compound: 17	Antiproliferative activity against human MV4-11 AML cells assessed as cell viability measured after 48 hrs by by multiplate reader method Antiproliferative activity against human MV4-11 AML cells assessed as cell viability measured after 48 hrs by by multiplate reader method	[PMID: 34942071]
NB-4	GI₅₀	19.5 nM Compound: 18	Antiproliferative activity against p53-null human NB4 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis Antiproliferative activity against p53-null human NB4 cells assessed as inhibition of cell growth incubated for 48 hrs by resazurin dye based fluorescence analysis	[PMID: 38224650]
RPMI-8226	EC₅₀	> 10000 nM Compound: 3b	Cytotoxicity against human RPMI-8226 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay Cytotoxicity against human RPMI-8226 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay	[PMID: 27754681]
RS4-11	EC₅₀	151.7 nM Compound: 3b	Cytotoxicity against human RS4-11 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay Cytotoxicity against human RS4-11 cells assessed as reduction in cell viability incubated for 48 hrs by Cell-titer-blue cell viability assay	[PMID: 27754681]
RS4-11	IC₅₀	81.97 nM Compound: 17	Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability measured after 48 hrs by multiplate reader method Antiproliferative activity against human RS4-11 cells assessed as reduction in cell viability measured after 48 hrs by multiplate reader method	[PMID: 34942071]
U-937	IC₅₀	161.4 nM Compound: 17	Antiproliferative activity against human U-937 cells assessed as reduction in cell viability after 48 hrs by MTT assay Antiproliferative activity against human U-937 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 34942071]

Molecular Weight

337.42

Formula

C₂₀H₂₃N₃O₂

CAS No.

2044701-99-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(/C=C/C1=CC=CC=C1)NCC2=CC=C(C=C2)C(NNCCC)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (14.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9637 mL	14.8183 mL	29.6367 mL
5 mM	0.5927 mL	2.9637 mL	5.9273 mL
10 mM	0.2964 mL	1.4818 mL	2.9637 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.28%

Select Batch:

Data Sheet (268 KB) SDS (252 KB)

COA (243 KB) HNMR (282 KB) RP-HPLC (234 KB) MS (230 KB)

Handling Instructions (2659 KB)

References

[1]. Moreno-Yruela C, Olsen CA. Determination of Slow-Binding HDAC Inhibitor Potency and Subclass Selectivity. ACS Med Chem Lett. 2022 Mar 16;13(5):779-785. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9637 mL	14.8183 mL	29.6367 mL	74.0916 mL
	5 mM	0.5927 mL	2.9637 mL	5.9273 mL	14.8183 mL
	10 mM	0.2964 mL	1.4818 mL	2.9637 mL	7.4092 mL

HDAC3-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HDAC3-IN-12044701-99-5HDACHistone deacetylasesEnzyme inhibitorhistone deacetylasescontinuous assayslow-binding kineticspreincubationInhibitorinhibitorinhibit

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HDAC3-IN-1

Customer Review

HDAC3-IN-1 Related Antibodies

1 Publications Citing Use of MCE HDAC3-IN-1

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HDAC Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

HDAC3-IN-1 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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