Hexestrol
Based on 2 publication(s) in Google Scholar
Hexestrol is a nonsteroidal synthetic estrogen, with a Ki of 0.06 and 0.06 nM for estrogen receptor alpha (ERα) and ERβ. Hexestrol can be used for the research of the diseases caused by estrogen deficiencym, and it also can increase the weight of cattle.
For research use only. We do not sell to patients.
- Purity: 99.79%
- CAS No.: 84-16-2
- Formula: C18H22O2
- Molecular Weight:270.37
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Endocrinology Compound Library
HY-L047
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Orally Active Compound Library
HY-L061
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FDA Approved & Pharmacopeial Drug Library
HY-L066
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Nuclear Receptor Compound Library
HY-L126
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Withdrawn Drug Compound Library
HY-L140
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Membrane Receptor-targeted Compound Library
HY-L150
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Multi-Target Compound Library
HY-L176
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Human Hormone Compound Library
HY-L211
Publications Citing Use of MedChemExpress (MCE) Hexestrol
More
Biological Activity
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ERα 0.06 nM (Ki) |
ERβ 0.06 nM (Ki) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Ishikawa | EC50 |
0.0001 μM
Compound: Hexestrol
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Agonist activity in Ishikawa human endometrial cells
Agonist activity in Ishikawa human endometrial cells
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10.1016/0960-894X(96)00165-5 |
| Ishikawa | EC50 |
0.1 nM
Compound: Hexestrol
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Agonist activity in Ishikawa human endometrial cells
Agonist activity in Ishikawa human endometrial cells
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10.1016/0960-894X(96)00165-5 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mus musculus (90-120 days, 25-50 g)[2]
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Dosage:3, 6 mg/kg (adopted the same dose used to increase weight gain in cattle)
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Administration:I.p. once daily for 30 days
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Result:Numerous follicles in different stages of development were found at the dose of 3 mg/kg in the ovaries.
Not detected the corpora lutea (CL) at the dose of 6 mg/kg.
Chemical Information
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CAS No. 84-16-2
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Appearance Solid
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Molecular Weight 270.37
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Formula C18H22O2
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Color White to off-white
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SMILES
CC[C@H](C1=CC=C(O)C=C1)[C@H](C2=CC=C(O)C=C2)CC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Cell Rep Methods
RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]2023 Oct 23;3(10):100599. PMID: 37797618 -
Int J Med Microbiol
Antibacterial and antibiofilm efficacy of repurposing drug hexestrol against methicillin-resistant Staphylococcus aureus. [Abstract]2023 Mar 28;313(2):151578. PMID: 37001448
Solvent & Solubility
DMSO : 100 mg/mL (369.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (7.69 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. HARDING FE, et, al. The oral use of hexestrol for estrogen deficiency. Am J Obstet Gynecol. 1946 May; 51: 660-5. [Content Brief]
[2]. Oliveira JM, et, al. Effects of hexestrol on mouse ovarian morphology and ovulation. Maturitas. 2008 Jun 20; 60(2): 153-7. [Content Brief]
[3]. Kuiper GG, et, al. Comparison of the ligand binding specificity and transcript tissue distribution of estrogen receptors alpha and beta. Endocrinology. 1997 Mar;138(3):863-70. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6986 mL | 18.4932 mL | 36.9864 mL | 92.4659 mL |
| 5 mM | 0.7397 mL | 3.6986 mL | 7.3973 mL | 18.4932 mL | |
| 10 mM | 0.3699 mL | 1.8493 mL | 3.6986 mL | 9.2466 mL | |
| 15 mM | 0.2466 mL | 1.2329 mL | 2.4658 mL | 6.1644 mL | |
| 20 mM | 0.1849 mL | 0.9247 mL | 1.8493 mL | 4.6233 mL | |
| 25 mM | 0.1479 mL | 0.7397 mL | 1.4795 mL | 3.6986 mL | |
| 30 mM | 0.1233 mL | 0.6164 mL | 1.2329 mL | 3.0822 mL | |
| 40 mM | 0.0925 mL | 0.4623 mL | 0.9247 mL | 2.3116 mL | |
| 50 mM | 0.0740 mL | 0.3699 mL | 0.7397 mL | 1.8493 mL | |
| 60 mM | 0.0616 mL | 0.3082 mL | 0.6164 mL | 1.5411 mL | |
| 80 mM | 0.0462 mL | 0.2312 mL | 0.4623 mL | 1.1558 mL | |
| 100 mM | 0.0370 mL | 0.1849 mL | 0.3699 mL | 0.9247 mL |
Hexestrol Related Classifications
HY-B1662 Related Classifications
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