L-Ascorbic acid 2-phosphate
Based on 10 publication(s) in Google Scholar
L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) is a long-acting vitamin C derivative that can stimulate collagen formation and expression. L-ascorbic acid 2-phosphate (2-Phospho-L-ascorbic acid) can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-ascorbic acid 2-phosphate increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation.
For research use only. We do not sell to patients.
- CAS No.: 23313-12-4
- Formula: C6H9O9P
- Molecular Weight:256.10
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) L-Ascorbic acid 2-phosphate
More- Adv Mater. 2025 Jul 14:e2501306. [Abstract]
- Autophagy. 2023 Feb;19(2):632-643. [Abstract]
- Adv Sci (Weinh). 2025 Nov 21:e08472. [Abstract]
- Adv Sci (Weinh). 2024 Dec;11(46):e2400502. [Abstract]
- Food Hydrocoll. 2026 Mar.
- Int J Biol Macromol. 2025 Dec 12;338(Pt 1):149672. [Abstract]
- Biomolecules. 2025 Nov 19;15(11):1623. [Abstract]
- J Biomed Mater Res A. 2026 Feb;114(2):e70031. [Abstract]
- Cell Transplant. 2025 Jan-Dec:34:9636897251376125. [Abstract]
- Eng Life Sci. 2025 Oct 13;25(10):e70050. [Abstract]
All Endogenous Metabolite Isoforms
More
Biological Activity
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Human Endogenous Metabolite |
L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect [1]. L-Ascorbic acid 2-phosphate (50 μM-250 μM) is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 μM AsA2-P and 10 nM dexamethasone (Dex), and 250 μM AsA2-P and 5 nM Dex[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human corneal endothelial cells (HCECs)
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Concentration:0.1 mM; 0.3 mM; 1.5 mM
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Incubation Time:2 to 3 weeks with medium exchange every 2 to 3 days
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Result:Stimulated HCEC cells growth.
Chemical Information
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CAS No. 23313-12-4
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Appearance Solid
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Molecular Weight 256.10
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Formula C6H9O9P
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Color Off-white to yellow
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SMILES
OC([C@H](O1)[C@H](CO)O)=C(OP(O)(O)=O)C1=O
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Synonyms
2-Phospho-L-ascorbic acid; LAA2P
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (10)
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Journal Impact Factor
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Most Recent
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Adv Mater
Piezoelectric Injectable Anti-Adhesive Hydrogel to Promote Endogenous Healing of Tendon Injuries. [Abstract]2025 Jul 14:e2501306. PMID: 40658817 -
Autophagy
Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis. [Abstract]2023 Feb;19(2):632-643. PMID: 35786294 -
Adv Sci (Weinh)
Targeted Inhibition of CD74+ Macrophages by Luteolin via CEBPB/P65 Signaling Ameliorates Osteoarthritis Progression. [Abstract]2025 Nov 21:e08472. PMID: 41268703 -
Adv Sci (Weinh)
Insufficient Mechanical Loading Downregulates Piezo1 in Chondrocytes and Impairs Fracture Healing Through ApoE-Induced Senescence. [Abstract]2024 Dec;11(46):e2400502. PMID: 39418070 -
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Int J Biol Macromol
Downregulation of the biological macromolecule Spondin-2 disrupts maternal-fetal immune tolerance and trophoblast invasion, driving unexplained recurrent pregnancy loss. [Abstract]2025 Dec 12;338(Pt 1):149672. PMID: 41391791 -
Biomolecules
Mitochondrial Imaging and Transcriptome Analysis of Bone Mesenchymal Stem Cells During Osteogenesis Under Different Culture Conditions. [Abstract]2025 Nov 19;15(11):1623. PMID: 41301543 -
J Biomed Mater Res A
Injectable Butyl Glycidyl Ether-Modified Methylcellulose Thermosensitive Hydrogel for Topical Delivery of Vancomycin Hydrochloride in the Treatment of Osteomyelitis. [Abstract]2026 Feb;114(2):e70031. PMID: 41586469 -
Cell Transplant
Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice. [Abstract]2025 Jan-Dec:34:9636897251376125. PMID: 41108126 -
Eng Life Sci
Comparative Study of Porcine Mesenchymal Stem Cells Behavior and Lipid Metabolism on Plant-Based Scaffolds and Two-Dimensional Systems for Cultivated Fat. [Abstract]2025 Oct 13;25(10):e70050. PMID: 41098339
Solvent & Solubility
1 M HCl : 25 mg/mL (97.62 mM; Need ultrasonic)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Shima N, et al. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate.Invest Ophthalmol Vis Sci. 2011 Nov 7;52(12):8711-7. [Content Brief]
[2]. Kurata S, et al. Epidermal growth factor inhibits transcription of type I collagen genes and production of type I collagen in cultured human skin fibroblasts in the presence and absence of L-ascorbic acid 2-phosphate, a long-acting vitamin C derivative.J Biol Chem. 1991 May 25;266(15):9997-10003. [Content Brief]
[3]. Kyllönen L, et al. Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro.Stem Cell Res Ther. 2013 Feb 15;4(1):17. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| 1 M HCl | 1 mM | 3.9047 mL | 19.5236 mL | 39.0472 mL | 97.6181 mL |
| 5 mM | 0.7809 mL | 3.9047 mL | 7.8094 mL | 19.5236 mL | |
| 10 mM | 0.3905 mL | 1.9524 mL | 3.9047 mL | 9.7618 mL | |
| 15 mM | 0.2603 mL | 1.3016 mL | 2.6031 mL | 6.5079 mL | |
| 20 mM | 0.1952 mL | 0.9762 mL | 1.9524 mL | 4.8809 mL | |
| 25 mM | 0.1562 mL | 0.7809 mL | 1.5619 mL | 3.9047 mL | |
| 30 mM | 0.1302 mL | 0.6508 mL | 1.3016 mL | 3.2539 mL | |
| 40 mM | 0.0976 mL | 0.4881 mL | 0.9762 mL | 2.4405 mL | |
| 50 mM | 0.0781 mL | 0.3905 mL | 0.7809 mL | 1.9524 mL | |
| 60 mM | 0.0651 mL | 0.3254 mL | 0.6508 mL | 1.6270 mL | |
| 80 mM | 0.0488 mL | 0.2440 mL | 0.4881 mL | 1.2202 mL |
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