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Homocarbonyltopsentin  (Synonyms: PK4C9)

Cat. No.: HY-117389 Purity: 99.88%
COA Handling Instructions

Molarity Calculator

Homocarbonyltopsentin (PK4C9) is a small-molecule TSL2-binding compound, binds to pentaloop conformations of TSL2 and promotes a shift to triloop conformations that display enhanced SMN2 exon 7 (E7) splicing with EC50 value of 16 μM.

For research use only. We do not sell to patients.

Homocarbonyltopsentin Chemical Structure

Homocarbonyltopsentin Chemical Structure

CAS No. : 172286-77-0

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 242 In-stock
Solution
10 mM * 1 mL in DMSO USD 242 In-stock
Solid
5 mg USD 220 In-stock
10 mg USD 350 In-stock
25 mg USD 730 In-stock
50 mg USD 1150 In-stock
100 mg USD 1850 In-stock
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Based on 1 publication(s) in Google Scholar

Homocarbonyltopsentin Related Antibodies

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  • Purity & Documentation

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Description

Homocarbonyltopsentin (PK4C9) is a small-molecule TSL2-binding compound, binds to pentaloop conformations of TSL2 and promotes a shift to triloop conformations that display enhanced SMN2 exon 7 (E7) splicing with EC50 value of 16 μM[1].

In Vitro

Homocarbonyltopsentin (PK4C9) (10-40 μM; 24 hours) shows an up to 5.2-fold decrease in the expression of E7-excluding SMN2 isoforms, and up to three-fold increase in E7-including isoforms in GM03813C cells[1].
Homocarbonyltopsentin (PK4C9) (40 μM; 24 hours) increased 1.5-fold SMN protein expression compared to GM03813C cells treated with DMSO[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Homocarbonyltopsentin Related Antibodies

RT-PCR[1]

Cell Line: GM03813C (SMA) cells; GM03814B fibroblasts cells
Concentration: 10-40 μM
Incubation Time: 24 hours
Result: E7 inclusion reached a maximum of 91% (25% increase, DMSO-treated cells).

Western Blot Analysis[1]

Cell Line: GM03813C (SMA) cells; GM03814B fibroblasts cells
Concentration: 40 μM
Incubation Time: 24 hours
Result: Increased SMN protein in SMA cells.
Molecular Weight

370.36

Formula

C21H14N4O3
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

O=C(C1=NC=C(C(C2=CNC3=C2C=CC=C3)=O)N1)C4=CNC5=C4C=CC(O)=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (337.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7001 mL 13.5004 mL 27.0008 mL
5 mM 0.5400 mL 2.7001 mL 5.4002 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (5.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.88%

COA (251 KB) HNMR (126 KB) RP-HPLC (170 KB) LCMS (129 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7001 mL 13.5004 mL 27.0008 mL 67.5019 mL
5 mM 0.5400 mL 2.7001 mL 5.4002 mL 13.5004 mL
10 mM 0.2700 mL 1.3500 mL 2.7001 mL 6.7502 mL
15 mM 0.1800 mL 0.9000 mL 1.8001 mL 4.5001 mL
20 mM 0.1350 mL 0.6750 mL 1.3500 mL 3.3751 mL
25 mM 0.1080 mL 0.5400 mL 1.0800 mL 2.7001 mL
30 mM 0.0900 mL 0.4500 mL 0.9000 mL 2.2501 mL
40 mM 0.0675 mL 0.3375 mL 0.6750 mL 1.6875 mL
50 mM 0.0540 mL 0.2700 mL 0.5400 mL 1.3500 mL
60 mM 0.0450 mL 0.2250 mL 0.4500 mL 1.1250 mL
80 mM 0.0338 mL 0.1688 mL 0.3375 mL 0.8438 mL
100 mM 0.0270 mL 0.1350 mL 0.2700 mL 0.6750 mL
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Homocarbonyltopsentin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Homocarbonyltopsentin172286-77-0PK4C9OthersInhibitorinhibitorinhibit

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