HPK1-IN-35
HPK1-IN-35 is a potent and selective HPK1 inhibitor with an IC50 value of 3.5 nM. HPK1-IN-35 decreases the expression of p-SLP76 and promotes IL-2 secretion.
For research use only. We do not sell to patients.
- CAS No.: 2935903-77-6
- Formula: C30H32N8O3S
- Molecular Weight:584.69
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
[1]|
HPK1 3.5 nM (IC50) |
In Vitro
HPK1-IN-35 (compound 31) (0.1, 0.3, 1, 3 µM; 1 h) decreases the expression of p-SLP76 stimulated by anti-CD3 antibody (1 µg/mL) in a dose-dependent manner[1].
HPK1-IN-35 (0-10 µM; 24 h) promotes IL-2 secretion in Jurkat cells in a dose-dependent manner with EC50 values of 1.19 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Jurkat cells
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Concentration:0.1, 0.3, 1, 3 µM
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Incubation Time:1 h
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Result:Dose-dependently suppressed the phosphorylation of SLP76 stimulated by anti-CD3 antibody in Jurkat cells with IC50 values of 1.04 µM.
Chemical Information
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CAS No. 2935903-77-6
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Molecular Weight 584.69
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Formula C30H32N8O3S
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SMILES
COC1=C(C=C2CN(CCC2=C1)C)NC3=NC4=C(C(NC5=CC=CC=C5S(=O)(N(C)C)=O)=N3)C(C6=CC=CN=C6)=CN4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HPK1-IN-35 Related Classifications
HY-149232 Related Classifications
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