CWP232228
Based on 7 publication(s) in Google Scholar
CWP232228, a highly potent selective Wnt/β-catenin signaling inhibitor, antagonizes binding of β-catenin to T-cell factor (TCF) in the nucleus. CWP232228 suppresses tumor formation and metastasis without toxicity through the inhibition of the growth of breast and liver cancer stem cells (CSCs).
For research use only. We do not sell to patients.
- Purity: 98.09%
- CAS No.: 1144044-02-9
- Formula: C33H34N7Na2O7P
- Molecular Weight:717.62
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) CWP232228
More- Cell Death Discov. 2022 Oct 1;8(1):404. [Abstract]
- Phytother Res. 2024 Sep;38(9):4675-4694. [Abstract]
- Sci Rep. 2026 Feb 20. [Abstract]
- Cell Signal. 2024 Mar:115:111039. [Abstract]
- Front Physiol. 2021 Mar 3;12:626248. [Abstract]
- Mol Carcinog. 2024 Nov;63(11):2158-2173. [Abstract]
- Research Square Preprint. 2020 Jun.
Biological Activity
Wnt/β-catenin[1]
CWP232228 (0.01-100 μM; 48 hours) inhibits cell proliferation with IC50 values are 2 and 0.8 μM in mouse (4T1) and human (MDA-MB-435) breast cancer cell lines, respectively[1].
CWP232228 (0.01-10 μM; 48 hours) inhibits cell proliferation with IC50s of 2.566, 2.630, and 2.596 μM in Hep3B, Huh7 and HepG2 cells, respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Mouse (4T1) and human (MDA-MB-435) breast cancer cell lines
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Concentration:0.01, 0.1, 1, 10, 100 μM
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Incubation Time:48 hours
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Result:IC50s were 2 and 0.8 μM for 4T1 and MDA-MB-435 cell lines, respectively.
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Cell Line:Hepatocellular carcinoma cell lines HepG2, Huh7, and Hep3B
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Concentration:0.01, 0.1, 0.5, 1, 5, 10 μM
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Incubation Time:48 hours
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Result:IC50s were 2.566, 2.630, and 2.596 μM for Hep3B, Huh7 and HepG2 cells, respectively.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:7-week-old female Balb/c and NOD/SCID mice bearing 4T1 or MDA-MB-435 cell tumors[1]
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Dosage:100 mg/kg
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Administration:Administered i.p.; daily; 21days for mice bearing 4T1 cell tumors, 60 days for mice bearing MDA-MB-435 cell tumors
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Result:Treatment resulted in a significant reduction in tumor volume.
Chemical Information
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CAS No. 1144044-02-9
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Appearance Solid
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Molecular Weight 717.62
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Formula C33H34N7Na2O7P
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Color White to off-white
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SMILES
C=CCN1CC(N([C@@H](CC2=CC=C(OP(O[Na])(O[Na])=O)C=C2)C(N(CC3=CC=CC4=CN(C)N=C43)C5)=O)[C@H]5N1C(NCC6=CC=CC=C6)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (7)
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Journal Impact Factor
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Most Recent
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Cell Death Discov
Sevoflurane induces microRNA-18a to delay rat neurodevelopment via suppression of the RUNX1/Wnt/β-catenin axis. [Abstract]2022 Oct 1;8(1):404. PMID: 36182925 -
Phytother Res
Carvacrol ameliorates skin allograft rejection through modulating macrophage polarization by activating the Wnt signalling pathway. [Abstract]2024 Sep;38(9):4675-4694. PMID: 39120138 -
Sci Rep
Involvement of AKT/β-catenin in Enhanced Osteogenesis by Cannabidiol in Bone Stromal Cells Harvested from Human Tori. [Abstract]2026 Feb 20. PMID: 41720800 -
Cell Signal
LAIR1-mediated resistance of hepatocellular carcinoma cells to T cells through a GSK-3β/β-catenin/MYC/PD-L1 pathway. [Abstract]2024 Mar:115:111039. PMID: 38199599 -
Front Physiol
ETS Proto-Oncogene 1 Suppresses MicroRNA-128 Transcription to Promote Osteogenic Differentiation Through the HOXA13/β-Catenin Axis. [Abstract]2021 Mar 3;12:626248. PMID: 33746773 -
Mol Carcinog
STAMBPL1, transcriptionally regulated by SREBP1, promotes malignant behaviors of hepatocellular carcinoma cells via Wnt/β-catenin signaling pathway. [Abstract]2024 Nov;63(11):2158-2173. PMID: 39150093 -
Solvent & Solubility
H2O : 62.5 mg/mL (87.09 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL (69.67 mM); Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (283 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Jang GB, et al. Wnt/β-Catenin Small-Molecule Inhibitor CWP232228 Preferentially Inhibits the Growth of Breast Cancer Stem-like Cells. Cancer Res. 2015 Apr 15;75(8):1691-702. [Content Brief]
[2]. Kim JY, et al. Oncotarget. 2016 Apr 12;7(15):20395-409. CWP232228 targets liver cancer stem cells through Wnt/β-catenin signaling: a novel therapeutic approach for liver cancer treatment. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.3935 mL | 6.9675 mL | 13.9350 mL | 34.8374 mL |
| 5 mM | 0.2787 mL | 1.3935 mL | 2.7870 mL | 6.9675 mL | |
| 10 mM | 0.1393 mL | 0.6967 mL | 1.3935 mL | 3.4837 mL | |
| 15 mM | 0.0929 mL | 0.4645 mL | 0.9290 mL | 2.3225 mL | |
| 20 mM | 0.0697 mL | 0.3484 mL | 0.6967 mL | 1.7419 mL | |
| 25 mM | 0.0557 mL | 0.2787 mL | 0.5574 mL | 1.3935 mL | |
| 30 mM | 0.0464 mL | 0.2322 mL | 0.4645 mL | 1.1612 mL | |
| 40 mM | 0.0348 mL | 0.1742 mL | 0.3484 mL | 0.8709 mL | |
| 50 mM | 0.0279 mL | 0.1393 mL | 0.2787 mL | 0.6967 mL | |
| 60 mM | 0.0232 mL | 0.1161 mL | 0.2322 mL | 0.5806 mL | |
| 80 mM | 0.0174 mL | 0.0871 mL | 0.1742 mL | 0.4355 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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