MSAB
Based on 56 publication(s) in Google Scholar
MSAB is a potent and selective inhibitor of Wnt/β-catenin signaling. MSAB binds to β-catenin and promotes its degradation, and specifically downregulates Wnt/β-catenin target genes. MSAB exhibits potent anti-tumor effects selectively on Wnt-dependent cancer cells.
For research use only. We do not sell to patients.
- Purity: 99.77%
- CAS No.: 173436-66-3
- Formula: C15H15NO4S
- Molecular Weight:305.35
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
Publications Citing Use of MedChemExpress (MCE) MSAB
More- Exploration. 2025 Jun 08.
- Cell Mol Immunol. 2024 Aug;21(8):856-872. [Abstract]
- Bioact Mater. 2026 May 13:64:455-470. [Abstract]
- Cancer Res. 2026 Jan 2;86(1):58-79. [Abstract]
- J Adv Res. 2025 Apr 27:S2090-1232(25)00282-6. [Abstract]
- Gut Microbes. 2025 Dec 31;17(1):2597626. [Abstract]
- Genes Dis. 2024 Jul 24;12(3):101382. [Abstract]
- Sci Adv. 2026 Feb 27;12(9):eaea3355. [Abstract]
- Cell Death Dis. 2025 Dec 31. [Abstract]
- Cell Mol Biol Lett. 2025 Jan 3;30(1):1. [Abstract]
- Cell Mol Biol Lett. 2023 Jul 28;28(1):61. [Abstract]
- Int J Biol Sci. 2022 Jun 21;18(10):4053-4070. [Abstract]
- Cell Commun Signal. 2024 Aug 7;22(1):391. [Abstract]
- Acta Biomater. 2026 Jan 26:S1742-7061(26)00061-9. [Abstract]
- Cell Death Discov. 2024 May 1;10(1):207. [Abstract]
- J Transl Med. 2025 Nov 13;23(1):1278. [Abstract]
- Br J Pharmacol. 2025 Sep 28. [Abstract]
- Environ Pollut. 2023 Sep 1:332:121931. [Abstract]
- J Ethnopharmacol. 2022 Jul 15;293:115261. [Abstract]
- J Agric Food Chem. 2021 Sep 15;69(36):10581-10591. [Abstract]
- J Environ Sci. 2025 Dec 31.
- Life Sci. 2025 Mar 18:123570. [Abstract]
- Organogenesis. 2025 Dec 31;21(1):2575622. [Abstract]
- Cells. 2023 May 30;12(11):1509. [Abstract]
- Stem Cells Transl Med. 2024 Mar 15;13(3):255-267. [Abstract]
- J Dent. 2026 Jun:169:106664. [Abstract]
- Eur J Pharmacol. 2025 May 15:995:177430. [Abstract]
- Int J Mol Sci. 2025 May 12;26(10):4630. [Abstract]
- Int J Mol Sci. 2024 Mar 3;25(5):2947. [Abstract]
- Mol Neurobiol. 2025 Jun;62(6):8013-8037. [Abstract]
- Int Dent J. 2026 Jan 23;76(2):109323. [Abstract]
- Mol Med Rep. 2026 Mar;33(3):89. [Abstract]
- Pestic Biochem Physiol. 2026 Jun:221:107178. [Abstract]
- Cancers (Basel). 2023 Mar 16;15(6):1801. [Abstract]
- iScience. 2026 Jun 15;29(7):116377. [Abstract]
- Biomedicines. 2024 Aug 12;12(8):1826. [Abstract]
- Biosci Rep. 2023 Mar 31;43(3):BSR20222429. [Abstract]
- FASEB J. 2023 Mar;37(3):e22821. [Abstract]
- Development. 2024 Nov 1;151(21):dev202899. [Abstract]
- Nitric Oxide. 2024 Nov 16:S1089-8603(24)00141-1. [Abstract]
- Pathol Res Pract. 2026 Mar:279:156364. [Abstract]
- Toxicol Appl Pharmacol. 2026 Mar:508:117706. [Abstract]
- Stem Cells Int. 2021 Nov 28;2021:5660927. [Abstract]
- Platelets. 2025 Dec;36(1):2498353. [Abstract]
- Oral Dis. 2025 Mar;31(3):741-755. [Abstract]
- Dose Response. 2026 Apr 7;24(2):15593258261440983. [Abstract]
- Discov Oncol. 2025 Jan 9;16(1):27. [Abstract]
- Gene. 2026 Jun 26:1008:150291. [Abstract]
- Mamm Genome. 2025 Dec 23;37(1):20. [Abstract]
- Int J Impot Res. 2025 Jul;37(7):519-526. [Abstract]
- SSRN. 2025 Dec 1.
- University of Otago. 2025.
- Res Sq. 2025 Apr 02.
- ESS Open Archive. 2024 June 12.
- Louisiana State University. 2024.
- Heliyon. 2024 Apr 26;10(9):e30104. [Abstract]
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Cell Proliferation/Viability Assay
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Cell Migration/Invasion Assay
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WB
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Histological Imaging/Staining
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WB
Biological Activity
Wnt/β-catenin[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
0.58 μM
Compound: 132; MSAB
|
Anticancer activity against human HCT-116 cells assessed as reduction in cell viability
Anticancer activity against human HCT-116 cells assessed as reduction in cell viability
|
[PMID: 33445154] |
MSAB (2-10 μM) selectively decreases cell viability of Wnt-dependent cells while showing little effect on Wnt-independent cells and normal human cells[1].
MSAB (0.01-10 μM; 20 h) inhibits T-cell factor (TCF) luciferase reporter activity in HCT116 cells[1].
MSAB (20 h) suppresses the Wnt3a-induced TOP-Luc activation and increases of active β-catenin levels in HEK293T cells[1].
MSAB (0.5-10 μM; 20 h) decreases mRNA and protein levels of endogenous Wnt target genes in HCT116 cells[1].
MSAB (5 μM; 16 h) induces degradation of β-catenin in a proteasome-dependent manner in HCT116 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MSAB (10-20 mg/kg; i.p. twice daily for 2 weeks) inhibits tumor growth of MMTV-Wnt1 transgenic mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Athymic nude mice (5-6 weeks) are injected HCT116, HT115, H23, or H460 cells[1]
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Dosage:10, 20 mg/kg
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Administration:I.p. daily for 2 weeks
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Result:Reduced the size and weight of various types of Wnt-dependent HCT116, HT115, and H23 tumors.
Chemical Information
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CAS No. 173436-66-3
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Appearance Solid
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Molecular Weight 305.35
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Formula C15H15NO4S
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Color White to off-white
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SMILES
O=C(C1=CC=CC(NS(=O)(C2=CC=C(C=C2)C)=O)=C1)OC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (56)
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Journal Impact Factor
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Most Recent
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MSAB purchased from MedChemExpress. Usage Cited in: Exploration. 2025 Jun 08.
Quantitative assessment of the impact of MSAB (10 μM; 48 h) on the RL-QN15-induced hESCs proliferation and viability.
MSAB purchased from MedChemExpress. Usage Cited in: Exploration. 2025 Jun 08.
Representative images demonstrate the effectiveness of RL-QN15 in repairing hESC scratches with and without MSAB (10 μM; 0–48 h).
MSAB purchased from MedChemExpress. Usage Cited in: Exploration. 2025 Jun 08.
Effect of MSAB (10 μM; 72 h) on the expression of the proliferation-related stemness-related protein CK19 in hESCs treated with 10 nM RL-QN15. GAPDH was used as an internal control.
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Cell Mol Immunol
Chaperone- and PTM-mediated activation of IRF1 tames radiation-induced cell death and the inflammatory response. [Abstract]2024 Aug;21(8):856-872. PMID: 38849539 -
Bioact Mater
Stage-specific biomimetic nanoparticles reprogram osteoblast-adipocyte equilibrium for targeted osteoporosis therapy. [Abstract]2026 May 13:64:455-470. PMID: 42181171 -
Cancer Res
SPP1 Drives Colorectal Cancer Liver Metastasis and Immunotherapy Resistance by Stimulating CXCL12 Production in Cancer-Associated Fibroblasts. [Abstract]2026 Jan 2;86(1):58-79. PMID: 41051794 -
J Adv Res
OTUD1 delays wound healing by regulating endothelial function and angiogenesis in diabetic mice. [Abstract]2025 Apr 27:S2090-1232(25)00282-6. PMID: 40300668
MSAB purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Apr 27:S2090-1232(25)00282-6. [Abstract]
HE staining images of skin wound tissue with MSAB (20 mg/kg, ip, every two days for two weeks).
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Gut Microbes
Microbial metabolite trimethylamine-N-oxide facilitates colorectal inflammation-cancer transformation by blocking lysosomal degradation of Wnt signaling. [Abstract]2025 Dec 31;17(1):2597626. PMID: 41376600 -
Genes Dis
Isoleucyl-tRNA synthetase 2 promotes pancreatic ductal adenocarcinoma proliferation and metastasis by stabilizing β-catenin. [Abstract]2024 Jul 24;12(3):101382. PMID: 40092487 -
Sci Adv
Inflamed vessel-anchored release of H2 across the blood-brain barrier for ischemic stroke neuroprotection. [Abstract]2026 Feb 27;12(9):eaea3355. PMID: 41739927 -
Cell Death Dis
CRX is an intrinsic suppressor of epithelial‒mesenchymal transition in retinal pigment epithelial cells: a promising therapeutic avenue for subretinal fibrosis. [Abstract]2025 Dec 31. PMID: 41469516 -
Cell Mol Biol Lett
Epigenetic modification regulates the ligamentum flavum hypertrophy through miR-335-3p/SERPINE2/β-catenin signaling pathway. [Abstract]2025 Jan 3;30(1):1. PMID: 39754051 -
Cell Mol Biol Lett
2023 Jul 28;28(1):61. PMID: 37501100 -
Int J Biol Sci
The TTYH3/MK5 Positive Feedback Loop regulates Tumor Progression via GSK3-β/β-catenin signaling in HCC. [Abstract]2022 Jun 21;18(10):4053-4070. PMID: 35844789 -
Cell Commun Signal
Concomitant targeting of FLT3 and SPHK1 exerts synergistic cytotoxicity in FLT3-ITD+ acute myeloid leukemia by inhibiting β-catenin activity via the PP2A-GSK3β axis. [Abstract]2024 Aug 7;22(1):391. PMID: 39113090 -
Acta Biomater
Functionalized layered double hydroxide coatings on biodegradable Zn-Mg alloy enhance osteogenesis via the Wnt/β-catenin and PI3K/Akt signaling pathways. [Abstract]2026 Jan 26:S1742-7061(26)00061-9. PMID: 41605408 -
Cell Death Discov
TM7SF2-induced lipid reprogramming promotes cell proliferation and migration via CPT1A/Wnt/β-Catenin axis in cervical cancer cells. [Abstract]2024 May 1;10(1):207. PMID: 38693136 -
J Transl Med
Exploring the role of cellular plasticity in metabolic dysfunction-associated steatosis and related molecular mechanisms. [Abstract]2025 Nov 13;23(1):1278. PMID: 41233849 -
Br J Pharmacol
Oral administration of low-molecular-weight heparin ameliorates colitis by enhancing the gut mucus barrier via microbial tryptophan metabolites. [Abstract]2025 Sep 28. PMID: 41016739 -
Environ Pollut
Screening of differentially methylated genes in skeletal fluorosis of rats with different types and involvement of aberrant methylation of Cthrc1. [Abstract]2023 Sep 1:332:121931. PMID: 37268221 -
J Ethnopharmacol
The Chinese medicine Fufang Zhenzhu Tiaozhi capsule protects against atherosclerosis by suppressing EndMT via modulating Akt1/β-catenin signaling pathway. [Abstract]2022 Jul 15;293:115261. PMID: 35447198 -
J Agric Food Chem
Glucomannan from Aloe vera Gel Promotes Intestinal Stem Cell-Mediated Epithelial Regeneration via the Wnt/β-Catenin Pathway. [Abstract]2021 Sep 15;69(36):10581-10591. PMID: 34432461 -
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Life Sci
MMP3 as a new target of Danshensu/tetramethylpyrazine derivative for attenuating cardiac fibrosis post-myocardial infarction. [Abstract]2025 Mar 18:123570. PMID: 40113078 -
Organogenesis
Mechanism of CGF in osteogenic differentiation of periodontal ligament stem cells through the Wnt pathway. [Abstract]2025 Dec 31;21(1):2575622. PMID: 41231879 -
Cells
β-Catenin Elicits Drp1-Mediated Mitochondrial Fission Activating the Pro-Apoptotic Caspase-1/IL-1β Signalosome in Aeromonas hydrophila-Infected Zebrafish Macrophages. [Abstract]2023 May 30;12(11):1509. PMID: 37296630 -
Stem Cells Transl Med
ISX-9 Promotes KGF Secretion From MSCs to Alleviate ALI Through NGFR-ERK-TAU-β-Catenin Signaling Axis. [Abstract]2024 Mar 15;13(3):255-267. PMID: 38159248 -
J Dent
2026 Jun:169:106664. PMID: 41921651 -
Eur J Pharmacol
TIGAR plays neuroprotective roles in MPP+/MPTP-induced Parkinson's disease by alleviating ferroptosis. [Abstract]2025 May 15:995:177430. PMID: 40015596 -
Int J Mol Sci
Polyphyllin I Inhibits the Metastasis of Cervical Cancer Through the Regulation of the β-Catenin Signaling Pathway. [Abstract]2025 May 12;26(10):4630. PMID: 40429774 -
Int J Mol Sci
Proanthocyanidins Ameliorate LPS-Inhibited Osteogenesis of PDLSCs by Restoring Lysine Lactylation. [Abstract]2024 Mar 3;25(5):2947. PMID: 38474198 -
Mol Neurobiol
Activation of NR2A-Wnt-TLR2 Signaling Axis in Satellite Glial Cells of the Dorsal Root Ganglion Contributes to Neuropathic Pain Induced by Nerve Injury in Diabetic Mice. [Abstract]2025 Jun;62(6):8013-8037. PMID: 39964585 -
Int Dent J
Meis1 Negatively Regulates Epithelial-Mesenchymal Transition via Wnt/β-catenin Pathway in Oral Submucous Fibrosis. [Abstract]2026 Jan 23;76(2):109323. PMID: 41579623 -
Mol Med Rep
Collagen III regulates the termination of liver regeneration by suppressing hepatocyte proliferation and promoting functional recovery
. [Abstract]2026 Mar;33(3):89. PMID: 41543156 -
Pestic Biochem Physiol
Avermectin disrupts blood-brain barrier integrity in zebrafish larvae via the Wnt/β-catenin signaling pathway. [Abstract]2026 Jun:221:107178. PMID: 42264772 -
Cancers (Basel)
PRAME Promotes Cervical Cancer Proliferation and Migration via Wnt/β-Catenin Pathway Regulation. [Abstract]2023 Mar 16;15(6):1801. PMID: 36980687
MSAB purchased from MedChemExpress. Usage Cited in: Cancers (Basel). 2023 Mar 16;15(6):1801. [Abstract]
MSAB (5 μM) attenuates the activity of Wnt3a and β-caten in SiHa cells.
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iScience
STRAP promotes hepatocellular carcinoma progression through activation of an STRAP/Wnt-β-catenin/STRAP positive feedback loop. [Abstract]2026 Jun 15;29(7):116377. PMID: 42338478 -
Biomedicines
Pyruvate Carboxylase Attenuates Myocardial Ischemia-Reperfusion Injury in Heart Transplantation via Wnt/β-Catenin-Mediated Glutamine Metabolism. [Abstract]2024 Aug 12;12(8):1826. PMID: 39200290 -
Biosci Rep
The Wnt/β-catenin pathway regulates inflammation and apoptosis in ventilator-induced lung injury. [Abstract]2023 Mar 31;43(3):BSR20222429. PMID: 36825682 -
FASEB J
GSK-3β/β-catenin pathway plays crucial roles in the regulation of NK cell cytotoxicity against myeloma cells. [Abstract]2023 Mar;37(3):e22821. PMID: 36794671 -
Development
Axin1 regulates tooth root development by inhibiting AKT1-mTORC1 activation and Shh translation in Hertwig's epithelial root sheath. [Abstract]2024 Nov 1;151(21):dev202899. PMID: 39344774 -
Nitric Oxide
KLF4's role in regulating nitric oxide production and promoting microvascular formation following ischemic stroke. [Abstract]2024 Nov 16:S1089-8603(24)00141-1. PMID: 39557151 -
Pathol Res Pract
Serine/arginine-rich splicing factor 1 inhibits ferroptosis and promotes glycolysis in endometrial cancer via activating mTOR and β-catenin. [Abstract]2026 Mar:279:156364. PMID: 41564687 -
Toxicol Appl Pharmacol
Histone deacetylase SIRT2 regulates the development and metastasis of tongue cancer via FZD1-mediated Wnt/β-catenin pathway. [Abstract]2026 Mar:508:117706. PMID: 41485500 -
Stem Cells Int
2021 Nov 28;2021:5660927. PMID: 34876908 -
Platelets
Platelet-derived extracellular vesicles promote hair follicle growth through β-catenin signaling pathway. [Abstract]2025 Dec;36(1):2498353. PMID: 40323125 -
Oral Dis
OSR1 suppresses oral squamous cell carcinoma proliferation and migration via the AXIN2/β-catenin pathway. [Abstract]2025 Mar;31(3):741-755. PMID: 39286942 -
Dose Response
Skeletal Stem Cells Rescue Radiation-Induced Osteogenic Precursor Cell Dysfunction via the Wnt/β-Catenin Signaling Pathway. [Abstract]2026 Apr 7;24(2):15593258261440983. PMID: 41960277 -
Discov Oncol
MUC5B regulates alterations in the immune microenvironment in nasopharyngeal carcinoma via the Wnt/β-catenin signaling pathway. [Abstract]2025 Jan 9;16(1):27. PMID: 39786598 -
Gene
Traumatic brain injury enhances fracture healing by upregulating VNN1 to activate the Wnt/β-catenin signaling pathway. [Abstract]2026 Jun 26:1008:150291. PMID: 42361997 -
Mamm Genome
Alantolactone curbs the malignant progression of bladder cancer partly via the HSP90AB1/LRP5/β-catenin axis. [Abstract]2025 Dec 23;37(1):20. PMID: 41436627 -
Int J Impot Res
Chronic prostatitis/chronic pelvic pain syndrome impairs erectile function by inducing apoptosis in a rat model of experimental autoimmune prostatitis. [Abstract]2025 Jul;37(7):519-526. PMID: 39169141 -
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Heliyon
Microcystin-leucine arginine induces the proliferation of cholangiocytes and cholangiocarcinoma cells through the activation of the Wnt/β-catenin signaling pathway. [Abstract]2024 Apr 26;10(9):e30104. PMID: 38720699
Solvent & Solubility
DMSO : 250 mg/mL (818.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2749 mL | 16.3747 mL | 32.7493 mL | 81.8733 mL |
| 5 mM | 0.6550 mL | 3.2749 mL | 6.5499 mL | 16.3747 mL | |
| 10 mM | 0.3275 mL | 1.6375 mL | 3.2749 mL | 8.1873 mL | |
| 15 mM | 0.2183 mL | 1.0916 mL | 2.1833 mL | 5.4582 mL | |
| 20 mM | 0.1637 mL | 0.8187 mL | 1.6375 mL | 4.0937 mL | |
| 25 mM | 0.1310 mL | 0.6550 mL | 1.3100 mL | 3.2749 mL | |
| 30 mM | 0.1092 mL | 0.5458 mL | 1.0916 mL | 2.7291 mL | |
| 40 mM | 0.0819 mL | 0.4094 mL | 0.8187 mL | 2.0468 mL | |
| 50 mM | 0.0655 mL | 0.3275 mL | 0.6550 mL | 1.6375 mL | |
| 60 mM | 0.0546 mL | 0.2729 mL | 0.5458 mL | 1.3646 mL | |
| 80 mM | 0.0409 mL | 0.2047 mL | 0.4094 mL | 1.0234 mL | |
| 100 mM | 0.0327 mL | 0.1637 mL | 0.3275 mL | 0.8187 mL |