Cat. No.: HY-N0211

Cyasterone

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Cyasterone, a natural EGFR inhibitor, mainly isolated from Ajuga decumbens Thunb (Labiatae). Cyasterone manifests anti-proliferation effect by induced apoptosis and cell cycle arrests. Cyasterone may serves as a therapeutic anti-tumor agent against human tumors.

For research use only. We do not sell to patients.

Purity: 99.58%
CAS No.: 17086-76-9
Formula: C₂₉H₄₄O₈
Molecular Weight:520.65

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Cyasterone Chemical Structure

CAS No.: 17086-76-9

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Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

IC50: EGFR^[1]

All EGFR Isoforms :

In Vitro

Cyasterone exerts cytotoxicity on multiple cell lines: HeLa (IC₅₀=77.24 μg/ml); HepG-2 (IC₅₀=52.03 μg/ml); MCF-7 (IC₅₀=82.07 μg/ml) and MCF-7 (IC₅₀=82.07 μg/ml). And It has none cytotoxicity (IC₅₀>400 μg/ml) on 3 types of carcinoma (HT-29, Caco-2, T47D) and 1 type of normal (NIH 3T3) cell lines^[1].
Cyasterone (0-60 μg/ml; 48 hours) causes a significantly decreasing of the cell proliferation. It inhibits cell growth as a dose-dependent manner, exhibits IC₅₀ values of 38.50 μg/ml and 32.96 μg/ml, respectively^[1].
Cyasterone (0-60 μg/ml; 24 hours) decreases p-EGFR, p-MEK, and p-mTOR as a dose-dependent manner in A549 cells, it affects the MAPK signaling pathway activities^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

A549 cells and MGC823 cells
Concentration:

0 μg/ml, 20 μg/ml, 40 μg/ml, 60 μg/ml
Incubation Time:

24 hours
Result:

Inhibited cell proliferation as a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:

A549 cells
Concentration:

0 μg/ml, 20 μg/ml, 40 μg/ml, 60 μg/ml
Incubation Time:

24 hours
Result:

Surpressed p-EGFR, p-MEK, and p-mTOR expression.

In Vivo

Cyasterone (intraperitoneal injection; 5 mg/kg, 10 mg/kg and 15 mg/kg; 21 days) has anti-proliferation effect in vivo and inhibits MGC823 cells xenografted tumor growth in vivo with few changes in body weights^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

MGC823 xenograft tumor in BALB/C-nu mice^[1]
Dosage:

5 mg/kg, 10 mg/kg and 15 mg/kg
Administration:

Intraperitoneal injection
Result:

Inhibited MGC823 xenograft tumor growth in vivo.

Chemical Information

CAS No. 17086-76-9
Appearance Solid
Molecular Weight 520.65
Formula C₂₉H₄₄O₈
Color White to off-white
SMILES

C[C@@]12[C@]([C@](C)([C@H](O)C[C@@H]3[C@@H](C)OC([C@H]3C)=O)O)([H])CC[C@@]1(O)C4=CC([C@]5([H])C[C@@H](O)[C@@H](O)C[C@]5(C)[C@@]4([H])CC2)=O
Structure Classification
- Steroids
Initial Source
- Plants
- Labiatae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

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Journal Impact Factor

J Ethnopharmacol

The protective mechanism of Tao Hong Si Wu decoction against breast cancer through regulation of EGFR/ERK1/2 signaling. [Abstract]

2024 Oct 5:332:118339. PMID: 38777083
Curr Med Chem

Cyasterone Improves Mitochondrial Function and Protects against Knee Osteoarthritis by Activating PPARγ. [Abstract]

2026 Mar 5. PMID: 41837479
Anticancer Res

Gossypol Inhibits GLI3-dependent SHH Signaling to Selectively Target SPOP-deficient Breast Cancer Cells. [Abstract]

2026 May;46(5):2317-2327. PMID: 42049345

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (192.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9207 mL

9.6034 mL

19.2068 mL

5 mM

0.3841 mL

1.9207 mL

3.8414 mL

10 mM

0.1921 mL

0.9603 mL

1.9207 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass

Concentration

Volume

Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage
mg/kg
Animal Weight (Per Animal)
g
Dosing Volume (Per Animal)
μL
Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lu X, et al. Anti-proliferation effects, efficacy of cyasterone in vitro and in vivo and its mechanism. Biomed Pharmacother. 2016 Dec;84:330-339. [Content Brief]

[2]. Lu X,et al. Anti-proliferation effects, efficacy of cyasterone in vitro and in vivo and its mechanism.Biomed Pharmacother. 2016 Dec;84:330-339. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9207 mL	9.6034 mL	19.2068 mL	48.0169 mL
	5 mM	0.3841 mL	1.9207 mL	3.8414 mL	9.6034 mL
	10 mM	0.1921 mL	0.9603 mL	1.9207 mL	4.8017 mL
	15 mM	0.1280 mL	0.6402 mL	1.2805 mL	3.2011 mL
	20 mM	0.0960 mL	0.4802 mL	0.9603 mL	2.4008 mL
	25 mM	0.0768 mL	0.3841 mL	0.7683 mL	1.9207 mL
	30 mM	0.0640 mL	0.3201 mL	0.6402 mL	1.6006 mL
	40 mM	0.0480 mL	0.2401 mL	0.4802 mL	1.2004 mL
	50 mM	0.0384 mL	0.1921 mL	0.3841 mL	0.9603 mL
	60 mM	0.0320 mL	0.1601 mL	0.3201 mL	0.8003 mL
	80 mM	0.0240 mL	0.1200 mL	0.2401 mL	0.6002 mL
	100 mM	0.0192 mL	0.0960 mL	0.1921 mL	0.4802 mL

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Cyasterone

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Publications Citing Use of MedChemExpress (MCE) Cyasterone

All EGFR Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Cyasterone Related Classifications

Cat. No.: HY-N0211