2. MAPK/ERK Pathway Apoptosis
  3. JNK p38 MAPK Apoptosis
  4. (+)-Isomenthone

(+)-Isomenthone is an enantiomer form of Menthone (HY-N2381). (+)-Isomenthone blocks TNF-α-triggered activation of the JNK and p38 MAPK pathways.(+)-Isomenthone inhibits TNF-α-mediated reductions in cell viability, increases in apoptosis, and downstream apoptotic events linked to pathway activation.(+)-Isomenthone protects human dermal fibroblasts against TNF-α-induced cell death under serum-deprived conditions.

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(+)-Isomenthone

(+)-Isomenthone Chemical Structure

CAS No. : 1196-31-2

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of (+)-Isomenthone:

(+)-Isomenthone Related Antibodies

Top Publications Citing Use of Products

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JNK1 JNK2 JNK3 JNK

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p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

(+)-Isomenthone is an enantiomer form of Menthone (HY-N2381). (+)-Isomenthone blocks TNF-α-triggered activation of the JNK and p38 MAPK pathways.(+)-Isomenthone inhibits TNF-α-mediated reductions in cell viability, increases in apoptosis, and downstream apoptotic events linked to pathway activation.(+)-Isomenthone protects human dermal fibroblasts against TNF-α-induced cell death under serum-deprived conditions[1].

In Vitro

(+)-Isomenthone (1-50 μM; 1-6 days) protects human dermal fibroblasts from TNF-α-induced cell death in a concentration- and time-dependent manner[1].
(+)-Isomenthone (50 μM; 6 days) significantly reduces TNF-α-induced apoptosis in human dermal fibroblasts, lowering the apoptotic cell population from 64% to 23% after 6 days of treatment[1].
(+)-Isomenthone (50 μM; 6 days) suppresses downstream apoptotic events in TNF-α-treated human dermal fibroblasts, including cytochrome c release, caspase-3 activation, and PARP cleavage, after 6 days of treatment[1].
(+)-Isomenthone (10-50 μM; 14h) does not modulate NF-κB activity in human dermal fibroblasts, either alone or in the presence of TNF-α[1].
(+)-Isomenthone (1-50 μM; 2h pretreatment, followed by TNF-α stimulation for 5-40min) inhibits TNF-α-induced phosphorylation of JNK and p38 MAPK (but not ERK) in human dermal fibroblasts, with significant suppression observed at 5, 10, 20, and 40 minutes after TNF-α stimulation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(+)-Isomenthone Related Antibodies

Cell Viability Assay[1]

Cell Line: human dermal fibroblasts (neonatal foreskin-derived)
Concentration: 1 μM (6 days); 10 μM (6 days); 50 μM (1-6 days)
Incubation Time: 1-6 days
Result: Inhibited TNF-α-induced cytotoxicity in a concentration- and time-dependent manner.
Increased cell viability to 92% relative to the TNF-α-treated group at day 6 with 50 μM.
Showed significant improvements in cell viability at all tested concentrations compared to TNF-α-only controls at day 6.
Maintained higher cell viability than the TNF-α-only group from day 3 onward with 50 μM, with statistically significant differences at days 3, 4, 5, and 6.

Apoptosis Analysis[1]

Cell Line: human dermal fibroblasts
Concentration: 50 μM
Incubation Time: 6 days
Result: Reduced the apoptotic cell population from 64% (TNF-α-only) to 23%, a statistically significant decrease.\nReduced the TUNEL-positive apoptotic cell population from 41% (TNF-α-only) to 8.3%, a statistically significant decrease.
Molecular Weight

154.25

Formula

C10H18O
CAS No.
Appearance

Liquid (Density: 0.881±0.06 g/cm3)

Color

Colorless to light yellow

SMILES

O=C1[C@@H](C(C)C)CC[C@@H](C)C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (648.30 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.4830 mL 32.4149 mL 64.8298 mL
5 mM 1.2966 mL 6.4830 mL 12.9660 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (16.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (16.21 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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+
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.13%

SDS (252 KB)

COA (248 KB) Elemental Analysis Report (106 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.4830 mL 32.4149 mL 64.8298 mL 162.0746 mL
5 mM 1.2966 mL 6.4830 mL 12.9660 mL 32.4149 mL
10 mM 0.6483 mL 3.2415 mL 6.4830 mL 16.2075 mL
15 mM 0.4322 mL 2.1610 mL 4.3220 mL 10.8050 mL
20 mM 0.3241 mL 1.6207 mL 3.2415 mL 8.1037 mL
25 mM 0.2593 mL 1.2966 mL 2.5932 mL 6.4830 mL
30 mM 0.2161 mL 1.0805 mL 2.1610 mL 5.4025 mL
40 mM 0.1621 mL 0.8104 mL 1.6207 mL 4.0519 mL
50 mM 0.1297 mL 0.6483 mL 1.2966 mL 3.2415 mL
60 mM 0.1080 mL 0.5402 mL 1.0805 mL 2.7012 mL
80 mM 0.0810 mL 0.4052 mL 0.8104 mL 2.0259 mL
100 mM 0.0648 mL 0.3241 mL 0.6483 mL 1.6207 mL
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(+)-Isomenthone Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(+)-Isomenthone1196-31-2JNKp38 MAPKApoptosisc-Jun N-terminal kinaseTNF-αapoptosiscytochrome cERKhuman dermal fibroblastsNF-κBPARPcaspase-3Inhibitorinhibitorinhibit

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