Propidium Iodide

Propidium Iodide purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Feb 28;17(4):3334-3345. [Abstract]

Customer Validation
Propidium iodide (PI) staining for cell death with GCFN and ferroptosis inhibitor ferrostatin-1 treatment for 48 h.

Propidium Iodide purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Apr 5;920:174830. [Abstract]

Customer Validation
RCMs were stained with Propidium Iodide (PI, 2 μg/mL; 2 h) and analyzed using a Nikon Ti2 microscope.

Propidium Iodide purchased from MedChemExpress. Usage Cited in: Molecules. 2022 Oct 9;27(19):6733. [Abstract]

Customer Validation
A549 cells were treated with erdafitinib (0, 6.25 and 12.5 μM, 24 h) and cell apoptosis was analyzed by flow cytometry and statistically analyzed. The Annexin V+/PI- and Annexin V+/PI+ cells were considered as early and late apoptotic cells, respectively, and the sum of the above two was calculated as apoptotic cells. Propidium iodide (PI, 50 μg/mL) was added for incubation for 30 min in the dark.

Propidium Iodide purchased from MedChemExpress. Usage Cited in: Thorac Cancer. 2022 May;13(9):1299-1310. [Abstract]

Customer Validation
KYSE450 and ECA109 cells after 96 h various transfections were washed three times in cold PBS, stained with Propidium iodide (PI, 50 μg/mL) and Annexin V‐FITC (25 μg/mL) for 20 min in the dark, and analyzed within 1 h.

Propidium Iodide purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Feb;49:102207. [Abstract]

Customer Validation
PAMs cells were washed with phosphate-buffered saline (PBS), and then incubated with the nuclear dye propidium iodide for 15 min. The nuclei were counterstained with propidium iodide (red). Fluorescent images were acquired with a confocal laser scanning microscope.

Propidium Iodide purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Feb;49:102207. [Abstract]

Customer Validation
PK-15^CD163 cells were washed with phosphate-buffered saline (PBS), and then incubated with the nuclear dye propidium iodide for 15 min. The nuclei were counterstained with propidium iodide (red). Fluorescent images were acquired with a confocal laser scanning microscope.

Propidium Iodide purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Aug 31;2021:5608133. [Abstract]

Customer Validation
The NP tissue slices are incubated with a culture medium containing 1 μg/ml Calcein AM and 1 μg/ml PI for one hour at 37°C. After washing with PBS, the slices are immediately viewed using an inverted confocal laser scanning microscope.

Propidium Iodide purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2018 Nov 28;10(11):3353-3370. [Abstract]

Customer Validation
KLE cells were stained by 25 μg/mL Propidium Iodide (PI) in fluorescence-activated cell sorting buffer, incubated cells for 30 min in dark environment at room temperature. Cell apoptosis was determined by Annexin V/PI double staining and percentages of cells in each phase (LL, viable; LR, early apoptotic; UL and UR, late apoptotic/necrotic cell) were calculated.