Carba1
Based on 1 Customer Validation
Carba1 is a bifunctional Carbazole (HY-D0204) derivative that activates nicotinamide phosphoribosyltransferase (NAMPT) and enhances NAD biosynthesis. Carba1 binds to colchicine site of tubulin, enhancing the anti-tumor effect of various chemotherapy drugs, such as Paclitaxel (HY-B0015). Carba1 exerts neuroprotective effect and can regulate cell energy metabolism. Carba1 can be used for the researches of cancer and chemotherapy-induced peripheral neuropathy (CIPN).
For research use only. We do not sell to patients.
- Purity : 98.23%
- CAS No.: 2635394-10-2
- Formula: C18H15ClN2
- Molecular Weight:294.78
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Carba1 (12 μM; 72 h) synergizes with Paclitaxel (HY-B0015), Docetaxel (HY-B0011), nab-Paclitaxel (HY-P99974), and Epothilone-B (HY-17029) to inhibit HeLa cell viability, reducing their GI50 values by 2.6, 3.8, 1.8 and 4.4 folds[1].
Carba1 (1-20 μM; 60 mins) enhances NAMPT activity in HeLa cells[1].
Carba1 (12 μM; 72 h) protects adult mouse DRG neurons from axonal degeneration induced by Paclitaxel, Cisplatin (HY-17394) and Bortezomib (HY-10227), reducing the degeneration index[1].
Carba1 (1-10 μM; 24 h) protects against Cisplatin-induced neurotoxicity and myelination impairment in rat DRG neurons[1].
Carba1 (12 μM, 2 h) regulates HeLa cell metabolism, increasing lactate, glutamate, ATP, and GTP levels, decreasing NAD+ levels, and enhancing glycolysis and glutaminolysis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Carba1 (60 mg/kg; i.v.; every 2 days for 10 days) does not promote tumor growth and does not inhibit the antitumor activity of Paclitaxel in tumor growth modeling[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Paclitaxel-induced rats neuropathy models[1]
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Dosage:50 mg/kg
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Administration:Intraperitoneally injection; on days 0, 1, 2, 4, 7, 9
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Result:Prevented tactile allodynia.
Reduced serum neurofilament light chain (NfL) levels.
Maintained intraepidermal nerve fiber (IENF) density.
Chemical Information
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CAS No. 2635394-10-2
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Appearance Solid
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Molecular Weight 294.78
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Formula C18H15ClN2
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Color White to off-white
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SMILES
CC1=CC(N2C=CC=C2)=C(C)C3=C1NC4=C3C=C(Cl)C=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (339.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.48 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.48 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.3924 mL | 16.9618 mL | 33.9236 mL | 84.8090 mL |
| 5 mM | 0.6785 mL | 3.3924 mL | 6.7847 mL | 16.9618 mL | |
| 10 mM | 0.3392 mL | 1.6962 mL | 3.3924 mL | 8.4809 mL | |
| 15 mM | 0.2262 mL | 1.1308 mL | 2.2616 mL | 5.6539 mL | |
| 20 mM | 0.1696 mL | 0.8481 mL | 1.6962 mL | 4.2405 mL | |
| 25 mM | 0.1357 mL | 0.6785 mL | 1.3569 mL | 3.3924 mL | |
| 30 mM | 0.1131 mL | 0.5654 mL | 1.1308 mL | 2.8270 mL | |
| 40 mM | 0.0848 mL | 0.4240 mL | 0.8481 mL | 2.1202 mL | |
| 50 mM | 0.0678 mL | 0.3392 mL | 0.6785 mL | 1.6962 mL | |
| 60 mM | 0.0565 mL | 0.2827 mL | 0.5654 mL | 1.4135 mL | |
| 80 mM | 0.0424 mL | 0.2120 mL | 0.4240 mL | 1.0601 mL | |
| 100 mM | 0.0339 mL | 0.1696 mL | 0.3392 mL | 0.8481 mL |
Carba1 Related Classifications
HY-179523 Related Classifications
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