JAK2-IN-6
Based on 1 publication(s) in Google Scholar
JAK2-IN-6, a multiple-substituted aminothiazole derivative, is a potent and selective JAK2 inhibitor with an IC50 of 22.86 μg/mL. JAK2-IN-6 shows no activity against JAK1 and JAK3. JAK2-IN-6 has anti-proliferative effect against cancer cells.
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- Purity : 97.07%
- CAS No.: 353512-04-6
- 화학식: C14H10ClN3OS2
- 분자량:335.83
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) JAK2-IN-6
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Biological Activity
제품 설명
IC50 & Target
[1]|
JAK2 22.86 μg/mL (IC50) |
In Vitro
JAK2-IN-6 (Compound B2; 6.3-50 μg/mL; 48 hours; PC-9, H1975 and PANC-1 cells) treatment exhibits significantly antiproliferative activity against all of these cancer cell lines, with IC50 values of 18.1 μg/mL, 58.3 μg/mL, 40.6 μg/mL against PC-9, H1975 and PANC-1, respectively[1].
JAK2-IN-6 (Compound B2), an intramolecular hydrogen bond is formed, holding the chlorothiophene substituent coplanar with the aminothiazole core. The chlorothiophene moiety is found to be located in the binding pocket adjacent to Val863 and Leu983, and extends towards the Asp994 of activation loop and the Gly993 of glycine-rich loop[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:PC-9, H1975 and PANC-1 cells
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Concentration:6.3 μg/mL, 12.5 μg/mL, 25 μg/mL, 50 μg/mL
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Incubation Time:48 hours
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Result:Exhibited significantly antiproliferative activity against all of these cancer cell lines.
Chemical Information
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CAS No. 353512-04-6
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Appearance Solid
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분자량 335.83
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화학식 C14H10ClN3OS2
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Color Light yellow to yellow
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SMILES
O=C(C1=C(N)N=C(NC2=CC=CC=C2)S1)C3=CC=C(Cl)S3
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Int Immunopharmacol
2025 Aug 28:161:115077. PMID: 40532325
용액&용해도
In Vitro:
DMSO : 60 mg/mL (178.66 mM; ultrasonic and adjust pH to 5 with HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
순도&문서
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.9777 mL | 14.8885 mL | 29.7770 mL | 74.4424 mL |
| 5 mM | 0.5955 mL | 2.9777 mL | 5.9554 mL | 14.8885 mL | |
| 10 mM | 0.2978 mL | 1.4888 mL | 2.9777 mL | 7.4442 mL | |
| 15 mM | 0.1985 mL | 0.9926 mL | 1.9851 mL | 4.9628 mL | |
| 20 mM | 0.1489 mL | 0.7444 mL | 1.4888 mL | 3.7221 mL | |
| 25 mM | 0.1191 mL | 0.5955 mL | 1.1911 mL | 2.9777 mL | |
| 30 mM | 0.0993 mL | 0.4963 mL | 0.9926 mL | 2.4814 mL | |
| 40 mM | 0.0744 mL | 0.3722 mL | 0.7444 mL | 1.8611 mL | |
| 50 mM | 0.0596 mL | 0.2978 mL | 0.5955 mL | 1.4888 mL | |
| 60 mM | 0.0496 mL | 0.2481 mL | 0.4963 mL | 1.2407 mL | |
| 80 mM | 0.0372 mL | 0.1861 mL | 0.3722 mL | 0.9305 mL | |
| 100 mM | 0.0298 mL | 0.1489 mL | 0.2978 mL | 0.7444 mL |
JAK2-IN-6 Related Classifications
HY-137756 Related Classifications
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