NSC647889
Based on 1 Customer Validation
NSC647889 is an apoptosis and autophagy inducer. NSC647889 induces apoptosis, inhibits mTOR pathway and abrogates DNA synthesis. NSC647889 triggers LC3-positive vesicle formation, modulates AKT and 4EBP1 phosphorylation and shows heightened caspase-3 activation in multicellular spheroids. NSC647889 can be used for the research of solid cancer tumour, head-neck carcinoma, and colorectal cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.28%
- CAS 番号: 1033931-92-8
- 分子式: C33H48N4OS
- 分子量:548.83
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
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Caspase-3 |
NSC-647889 (2-20 μM; 4-10 h) potently induces apoptosis in ~80% of HCT116 colon carcinoma multicellular spheroid cells (excluding hypoxic core cells) at 10 μM, reduces spheroid cell clonogenicity to ~1% at 20 μM, and shows a ~2.5-fold lower viability reduction potency in spheroids compared to monolayer cultures[2].
NSC-647889 (10 μM; 1 h) increases Ca2+/calmodulin-dependent calcineurin activity in HCT116 colon carcinoma cells[2].
NSC-647889 (1.25-10 μM; 1 h) increases intracellular calcium levels in HCT116 colon carcinoma cells in a dose-dependent manner, with an 8-fold increase over control at 7.5 μM after 1 h incubation[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116 colon carcinoma multicellular spheroids (MCS) and monolayer cultures
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Concentration:2 μM; 5 μM; 10 μM; 20 μM
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Incubation Time:4 h; 6 h; 8 h; 10 h
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Result:Induced apoptosis in ~80% of HCT116 spheroid cells, with apoptosis restricted to cells 0-100 µm from the spheroid surface.
Reduced clonogenicity of cells dispersed from treated spheroids to <5% of solvent-treated controls at 10 μM, and to ~1% at 20 μM.
Showed a ~2.5-fold difference in viability reduction potency between monolayer and spheroid cultures, with spheroids showing lower sensitivity.
Increased apoptosis induction (ccK18 levels) with higher concentrations in both culture systems, with monolayer cultures showing stronger apoptosis at lower doses.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:SCID mice[2]
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Dosage:5 mg/kg
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Administration:s.c.; single dose
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Result:Increased plasma caspase-cleaved K18 (ccK18) levels significantly compared to vehicle control.
Increased total K18 levels significantly compared to vehicle control.
化学情報
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CAS 番号 1033931-92-8
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性状 Solid
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分子量 548.83
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分子式 C33H48N4OS
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Color White to off-white
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SMILES
C[C@@]12[C@]3([H])[C@](CCC1C[C@H](CC2)NC(NC(C4=NC=CS4)CC5=CC=CC=C5)=O)([H])[C@@]6([H])[C@](CC3)([C@H](CC6)N(C)C)C
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 2.2 mg/mL (4.01 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8221 mL | 9.1103 mL | 18.2206 mL | 45.5514 mL |
NSC647889 関連分類
HY-176761 関連分類
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