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製品番号: HY-N21945

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Castalin

Castalin is a polyphenolic anticancer compound isolated and identified from the bark or nut shells of Castanea sativa Mill. Castalin induces DNA damage by increasing ROS to activate CHK1, while recruiting 53BP1/RIF1 and activating DNA-PKcs to initiate the NHEJ pathway for DNA repair. Castalin can be used in research related to cervical cancer and breast cancer.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 19086-75-0
分子式: C₂₇H₂₀O₁₈
分子量:632.44

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

Castalin 構造式

CAS 番号: 19086-75-0

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性
- 製品説明
- IC₅₀ & Target
- Cellular Effect
- 体外実験
化学情報
純度とドキュメンテーション
参考文献
Calculators
Help & FAQs
関連分類
Bioz

生物活性

製品説明

IC₅₀ & Target

^[1]

Chk1

Checkpoint Kinase (Chk) アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC50	25.13 μg/mL	Cytotoxicity against human cervix adenocarcinoma HeLa cells assessed as reduction in cell viability incubated for 72 hrs by sulforhodamine B assay. Cytotoxicity against human cervix adenocarcinoma HeLa cells assessed as reduction in cell viability incubated for 72 hrs by sulforhodamine B assay.	41009002
MCF7	IC50	16.1 μg/mL	Cytotoxicity against hormone-dependent human breast carcinoma MCF-7 cells assessed as reduction in cell viability incubated for 72 hrs by sulforhodamine B assay. Cytotoxicity against hormone-dependent human breast carcinoma MCF-7 cells assessed as reduction in cell viability incubated for 72 hrs by sulforhodamine B assay.	41009002
MDA-MB-231	IC50	5.2 μg/mL	Cytotoxicity against triple-negative human breast cancer MDA-MB-231 cells assessed as reduction in cell viability incubated for 72 hrs by sulforhodamine B assay. Cytotoxicity against triple-negative human breast cancer MDA-MB-231 cells assessed as reduction in cell viability incubated for 72 hrs by sulforhodamine B assay.	41009002

体外実験

Anti-cancerOxidative StressCell Cycle Regulation

Anti-cancer

Castalin (0.5-124 µg/mL; 72 h) induces dose-dependent cytotoxicity in HeLa, MCF-7 and MDA-MB-231 cancer cell lines, with IC₅₀ values of 25.13 µg/mL, 16.1 µg/mL and 5.2 µg/mL, respectively^[2].
Castalin (62-124 µg/mL; 1-6 h) induces dose- and time-dependent DNA double-strand breaks in HeLa cells, and this effect is detectable via γH2AX foci^[2].
Castalin (124 μg/mL; 3 h, followed by elution and recovery for 12-24 h) exerts an effect in HeLa cells that reduces γH2AX foci over time after elution, indicating the initiation of DNA repair^[2].
Castalin (31-124 μg/mL; 3 h) modulates transcriptional programs, downregulates homologous recombination-related genes, and upregulates tumor microenvironment-related pathways in HeLa cells. When combined with SRA737 (1 μM), it exerts a stronger effect on upregulating the phosphorylation level of CHK1 S345 than either agent used alone^[2].

Oxidative Stress

Anti-cancer

Castalin (31-124 μg/mL; 1-3 h) induces phosphorylation of CHK1 kinase at Ser345, triggers reactive oxygen species (ROS) production, significantly increases the number of 53BP1 and RIF1 foci, upregulates phosphorylation of DNA-PKcs at S2056, and does not induce phosphorylation of RPA32 protein^[2].

Anti-cancer

Cell Cycle Regulation

Combination treatment with Castalin (7.7 μg/mL; 6-72 h) and 1 μM SRA737 (HY-18958) synergistically enhances the cytotoxicity of CHK1 inhibitors and induces mitotic arrest in HeLa, MCF-7 and MDA-MB-231 cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

HeLa, MCF-7 cells
Concentration:

7.7, 15.5, 31, 62, 124 μg/mL
Incubation Time:

72 h
Result:

Decreased the viability of tumor cells in a dose-dependent manner.

Cell Viability Assay^[2]

Cell Line:

MDA-MB-231 cells
Concentration:

0.5, 1, 2, 4, 8 μg/mL
Incubation Time:

72 h
Result:

Strongly decreased the viability of the triple-negative breast cancer cell line in a dose-dependent manner.

Immunofluorescence^[2]

Cell Line:

HeLa cells
Concentration:

62, 84, 124 μg/mL
Incubation Time:

1, 3, 6 h
Result:

Increased the formation of intracellular γH2AX foci in a dose- and time-dependent manner, inducing DNA damage.

Western Blot Analysis^[2]

Cell Line:

HeLa cells
Concentration:

31, 62, 124 μg/mL
Incubation Time:

3 h
Result:

Dose-dependently increased the phosphorylation activation of the key checkpoint kinase CHK1 at Ser345.

Immunofluorescence^[2]

Cell Line:

HeLa cells
Concentration:

124 µg/mL
Incubation Time:

3 h
Result:

Significantly increased the number of 53BP1 and RIF1 foci involved in the Non-Homologous End Joining (NHEJ) repair pathway, and significantly up-regulated the phosphorylation of DNA-PKcs at S2056.

Western Blot Analysis^[2]

Cell Line:

HeLa cells
Concentration:

124 μg/mL
Incubation Time:

1, 2, 3 h
Result:

Failed to induce the phosphorylation of RPA32, a marker of the Homologous Recombination (HR) repair pathway.

Cell Viability Assay^[2]

Cell Line:

HeLa, MCF-7, MDA-MB-231 cells
Concentration:

7.7 μg/mL (combined with SRA737: 0.06, 0.125, 0.25, 0.5, 1 μM)
Incubation Time:

72 h
Result:

Significantly enhanced the cytotoxic effect of the CHK1 inhibitor SRA737, synergistically decreasing the survival rate of the three cancer cell lines compared to monotherapy.

Related Protocols :

化学情報

CAS 番号 19086-75-0
分子量 632.44
分子式 C₂₇H₂₀O₁₈
SMILES

OC(C(O1)C2[C@@]([H])(O)C3=C(O)C(O)=C(O)C4=C3C(O2)=O)C(CO)OC(C5=CC(O)=C(O)C(O)=C5C6=C(O)C(O)=C(O)C4=C6C1=O)=O
Structure Classification
- Phenols
- Polyphenols
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Lampire O, et al. Polyphenols isolated from the bark of castanea sativa Mill. chemical structures and auto-association in honour of professor G. H. Neil Towers 75th birthday. Phytochemistry 1998, 49, 623–631,

[2]. D'Angelo M, et al. Castalin Induces ROS Production, Leading to DNA Damage and Increasing the Activity of CHK1 Inhibitor in Cancer Cell Lines. Antioxidants (Basel, Switzerland). 2025 Sep 08;14(9):1096.

Calculators

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

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生物活性
- 製品説明
- IC₅₀ & Target
- 体外実験
化学情報
純度とドキュメンテーション
参考文献

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Castalin

Checkpoint Kinase (Chk) アイソフォーム固有の製品をすべて表示

生物活性

製品説明

IC50 & Target

Cellular Effect

体外実験

化学情報

純度とドキュメンテーション

参考文献

Calculators

Help & FAQs

Castalin 関連分類

HY-N21945 関連分類

Bioz

Keywords

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