LO-4-25
Based on 1 Customer Validation
LO-4-25 is a CUL4DCAF16 based covalent PROTAC-like degrader. LO-4-25 induces degradation of the androgen receptor (AR) and androgen receptor truncation variant AR-V7. LO-4-25 covalently targets cysteine residues on CUL4DCAF16 to mediate degradation of neo-substrates. LO-4-25 triggers proteasome-dependent degradation of target proteins via CUL4DCAF16-mediated ubiquitination. LO-4-25 can be used for the research of androgen-independent prostate cancer.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.68%
- 分子式: C32H37ClN6O5S
- 分子量:653.19
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
製品説明
体外実験
LO-4-25 (1-10 μM; 24 h) potently degrades both AR and AR-V7 in 22Rv1 cells in a CUL4DCAF16-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:22Rv1 androgen-independent prostate cancer cells
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Concentration:1 μM, 10 μM
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Incubation Time:24 h
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Result:Induced robust degradation of both AR and AR-V7 in 22Rv1 cells.
Completely attenuated degradation of AR and AR-V7 in 22Rv1 cells with DCAF16 knockdown via siRNA transfection.
化学情報
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性状 Solid
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分子量 653.19
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分子式 C32H37ClN6O5S
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Color Off-white to light yellow
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SMILES
O=C(NC1=CC(Cl)=CN=C1)/C=C/C(N2CCN(CC2)C(CCCCCOC3=CC=C(C=C3)C4=CSC(N5CCOCC5)=N4)=O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (153.09 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
体内:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5309 mL | 7.6547 mL | 15.3095 mL | 38.2737 mL |
| 5 mM | 0.3062 mL | 1.5309 mL | 3.0619 mL | 7.6547 mL | |
| 10 mM | 0.1531 mL | 0.7655 mL | 1.5309 mL | 3.8274 mL | |
| 15 mM | 0.1021 mL | 0.5103 mL | 1.0206 mL | 2.5516 mL | |
| 20 mM | 0.0765 mL | 0.3827 mL | 0.7655 mL | 1.9137 mL | |
| 25 mM | 0.0612 mL | 0.3062 mL | 0.6124 mL | 1.5309 mL | |
| 30 mM | 0.0510 mL | 0.2552 mL | 0.5103 mL | 1.2758 mL | |
| 40 mM | 0.0383 mL | 0.1914 mL | 0.3827 mL | 0.9568 mL | |
| 50 mM | 0.0306 mL | 0.1531 mL | 0.3062 mL | 0.7655 mL | |
| 60 mM | 0.0255 mL | 0.1276 mL | 0.2552 mL | 0.6379 mL | |
| 80 mM | 0.0191 mL | 0.0957 mL | 0.1914 mL | 0.4784 mL | |
| 100 mM | 0.0153 mL | 0.0765 mL | 0.1531 mL | 0.3827 mL |
LO-4-25 関連分類
HY-176067 関連分類
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