Muraymycin D2
Muraymycin D2 is a peptidyl nucleoside natural product derived from Streptomyces spp., possessing competitive translocase I (MraY) inhibitory activity and antibacterial activity. Muraymycin D2 competitively binds to the nucleotide-binding pocket of the MraY substrate via its nucleoside moiety, and upon binding, induces a conformational change in MraY, blocking lipid I synthesis and inhibiting peptidoglycan biosynthesis. Muraymycin D2 exhibits a Ki of 7.6 nM against Bacillus subtilis and an IC50 of 0.39 nM against Staphylococcus aureus. Muraymycin D2 shows antibacterial activity against Escherichia coli and is capable of breaking persistence in chlamydial infection. Muraymycin D2 can be used for research on bacterial infection-related studies, such as chlamydial infection.
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- CAS 番号: 474328-38-6
- 分子式: C37H61N11O16
- 分子量:915.94
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| HEp-2 | MIC |
128 μg/mL
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Minimal inhibitory concentration of Muraymycin D2 against C. trachomatis D/UW-3/CX in HEp-2 cells, inducing formation of large aberrant bodies, with compound added at 2 h post infection and effects analyzed at 30 h post infection by fluorescence microscopy.
Minimal inhibitory concentration of Muraymycin D2 against C. trachomatis D/UW-3/CX in HEp-2 cells, inducing formation of large aberrant bodies, with compound added at 2 h post infection and effects analyzed at 30 h post infection by fluorescence microscopy.
|
antibiotics13050421 |
体外実験
Muraymycin D2 (MRY D2) induces enlarged abnormal cells in C. trachomatis D/UW-3/CX, with an MIC of 128 µg/mL[2].
Muraymycin D2 (0.05-0.5 µM; 90 min) inhibits the enzymatic activity of purified C. pneumoniae MraY[2].
Muraymycin D2 (128 µg/mL; 18 h) eradicates persistent C. trachomatis D/UW-3/CX cells in PEN G-induced infection; when applied at the mid-stage, it reduces inclusions in active C. trachomatis D/UW-3/CX infection[2].
Muraymycin D2 exhibits non-Michaelis-Menten kinetics and substrate inhibition with Mur29, giving Km = 41 μM and kcat = 4.0 min−1; it also exhibits non-Michaelis-Menten kinetics and substrate inhibition when reacted with Mur28, with Km = 170 μM and kcat = 5.2 min−1[3].
Muraymycin D2 is regioselectively phosphorylated by the Mg·ATP-dependent 3''-phosphotransferase Mur28 to generate D2-PHOS; the phosphorylated muraymycin D2 exhibits significantly reduced inhibitory activity against S. aureus MraY[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEp-2 cells infected with C. trachomatis D/UW-3/CX
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Concentration:128 µg/mL
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Incubation Time:18 h (added at 12 hpi, analyzed at 30 hpi)
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Result:Resulted in a 70% reduction in the number of inclusions.\nObserved a 75% reduction in inclusions, of which 83% contained EBs/RBs and 8% had a mixed phenotype.
化学情報
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CAS 番号 474328-38-6
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分子量 915.94
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分子式 C37H61N11O16
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SMILES
NC[C@H]([C@H]([C@H]1O)O)O[C@H]1O[C@H]([C@@]2([H])O[C@@H](N3C(NC(C=C3)=O)=O)[C@@H]([C@@H]2O)O)[C@@H](C(O)=O)NCCCNC([C@H](CC(C)C)NC([C@H]([C@@]4([H])NC(N)=NCC4)NC(N[C@H](C(O)=O)C(C)C)=O)=O)=O
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Structure Classification
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Initial Source
Streptomyces spp.
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Muraymycin D2 関連分類
HY-N21878 関連分類
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