Ostruthin
Based on 1 Customer Validation
Ostruthin is a natural coumarin compound with bacterial and antimycobacterial activities. Ostruthin inhibits the growth of mycobacteria and Staphylococcus aureus. Ostruthin suppresses vascular smooth muscle cell proliferation as well as de novo cellular DNA synthesis. Ostruthin is applicable to research related to mycobacterial infections and cardiovascular and cerebrovascular diseases.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 98.84%
- CAS 番号: 148-83-4
- 分子式: C19H22O3
- 分子量:298.38
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保管条件:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
製品説明
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| VSMC | IC50 |
11 μM
Compound: 7
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Antiproliferative activity against serum-stimulated primary rat aortic VSMC after 30 mins by resazurin conversion assay
Antiproliferative activity against serum-stimulated primary rat aortic VSMC after 30 mins by resazurin conversion assay
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[PMID: 21627108] |
| VSMC | IC50 |
17 μM
Compound: 7
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Antiproliferative activity in primary rat aortic VSMC assessed as inhibition of serum-induced DNA synthesis pretreated 30 mins prior serum addition measured after 48 hrs by BrdU incorporation assay
Antiproliferative activity in primary rat aortic VSMC assessed as inhibition of serum-induced DNA synthesis pretreated 30 mins prior serum addition measured after 48 hrs by BrdU incorporation assay
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[PMID: 21627108] |
体外実験
Ostruthin (72-120 h) potently inhibits the proliferation of various rapidly growing mycobacterial strains in vitro. Its minimum inhibitory concentration (MIC) against Mycobacterium abscesus, M. aurum, M. fortuitum, M. phlei and M. smegmatis ranges from 3.4 to 107.4 μM[1].
Ostruthine (1×10-4-1×10-3 M) potently inhibits the growth and cellular oxygen consumption of Staphylococcus aureus strain BS 270[2].
Ostruthin (Compound 7) (30 μM; 48 h) potently inhibits 10% serum-induced proliferation of rat aortic vascular smooth muscle cells, with an IC50 of 11 μM[3].
Ostruthin (1-30 μM; 20 h) concentration-dependently inhibits de novo DNA synthesis induced by 10% serum in rat aortic vascular smooth muscle cells, with an IC50 of 17 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:rat aortic vascular smooth muscle cells (VSMC)
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Concentration:30 μM
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Incubation Time:48 h (with 30 min pretreatment)
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Result:Inhibited serum-induced VSMC proliferation with an IC50 of 11 μM.
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- MTT Cell Proliferation Assay
化学情報
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CAS 番号 148-83-4
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性状 Solid
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分子量 298.38
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分子式 C19H22O3
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Color White to off-white
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SMILES
C/C(C)=C\CC/C(C)=C/CC1=C(O)C=C2C(C=CC(O2)=O)=C1
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別名
Ostruthine
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
純度とドキュメンテーション
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データシート (268 KB)
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SDS (458 KB)
- English - EN (458 KB)
- Français - FR (458 KB)
- Deutsch - DE (458 KB)
- Norwegian - NO (458 KB)
- Español - ES (458 KB)
- Swedish - SV (458 KB)
- Italian - IT (458 KB)
- Korean - KR (458 KB)
- Portuguese - PT (458 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Schinkovitz A, et al. Ostruthin: an antimycobacterial coumarin from the roots of Peucedanum ostruthium. Planta Med. 2003;69(4):369-371. [Content Brief]
[3]. Joa H, et al. Identification of ostruthin from Peucedanum ostruthium rhizomes as an inhibitor of vascular smooth muscle cell proliferation. J Nat Prod. 2011;74(6):1513-1516. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Ostruthin 関連分類
HY-W506116 関連分類
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