Cat. No.: HY-N0802

Tenuigenin

(Synonyms: Senegenin)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Tenuigenin is a major active component isolated from the root of the Chinese herb Polygala tenuifolia. Tenuigenin protects against S.aureus-induced pneumonia by inhibiting NF-κB activation. Tenuigenin has anti-inflammatory effect.

For research use only. We do not sell to patients.

Purity: 99.92%
CAS No.: 2469-34-3
Formula: C₃₀H₄₅ClO₆
Molecular Weight:537.13

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Tenuigenin Chemical Structure

CAS No.: 2469-34-3

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Sample (0.1mg)

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(In Japan)

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

NF-κB

All NF-κB Isoforms :

In Vivo

Tenuigenin (2, 4, 8 mg/kg) significantly attenuates S.aureus-induced lung histopathological changes. Tenuigenin also inhibits lung wet/dry (W/D) ratio, and inflammatory cytokines TNF-α and IL-1β production in Male BALB/c mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 2469-34-3
Appearance Solid
Molecular Weight 537.13
Formula C₃₀H₄₅ClO₆
Color White to off-white
SMILES

C[C@@]([C@](CC1)([H])[C@]2(C)C(O)=O)(C[C@H](O)[C@@H]2O)[C@](C[C@H](CCl)C3=C4CC[C@]5(C(O)=O)[C@@]3([H])CC(C)(C)CC5)([H])[C@]41C
Synonyms

Senegenin
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (3)

All Cell Proliferation/Viability AssayIFFlow CytometryWBCell Migration/Invasion Assay

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Journal Impact Factor

J Ethnopharmacol

TMT-based quantitative proteomics reveals the protective mechanism of tenuigenin after experimental intracerebral hemorrhage in mice. [Abstract]

2024 Jan 30;319(Pt 2):117213. PMID: 37739103
Hereditas

Investigating the effect of tenuigenin on LPS-induced HPMEC dysfunction by inhibiting SRC activation based on network pharmacology and molecular docking. [Abstract]

2025 Sep 29;162(1):196. PMID: 41024189

Tenuigenin purchased from MedChemExpress. Usage Cited in: Hereditas. 2025 Sep 29;162(1):196. [Abstract]

CCK8 was used to detect the viability of HPMECs treated with Tenuigenin at 0, 0.5, 1, 2, 4, 8, and 16 µg/mL.

Tenuigenin purchased from MedChemExpress. Usage Cited in: Hereditas. 2025 Sep 29;162(1):196. [Abstract]

EdU staining was applied to measure the EdU-positive cells co-treated with Tenuigenin (0, 1, and 4 µg/mL) and LPS (1 µg/mL).

Tenuigenin purchased from MedChemExpress. Usage Cited in: Hereditas. 2025 Sep 29;162(1):196. [Abstract]

Flow cytometry was employed to detect apoptosis co-treated with Tenuigenin (0, 1, and 4 µg/mL) and LPS (1 µg/mL).

Tenuigenin purchased from MedChemExpress. Usage Cited in: Hereditas. 2025 Sep 29;162(1):196. [Abstract]

Western blot was applied to examine the protein expression of VE-cadherin and ZO-1 co-treated with Tenuigenin (0, 1, and 4 µg/mL) and LPS (1 µg/mL).

Tenuigenin purchased from MedChemExpress. Usage Cited in: Hereditas. 2025 Sep 29;162(1):196. [Abstract]

The migrated cells were determined by transwell assay treaed with Tenuigenin.
Cancer Chemother Pharmacol

Tenuigenin inhibits osteosarcoma growth via CIP2A/PP2A/NF-κB axis. [Abstract]

2024 Dec 30;95(1):15. PMID: 39739023

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (186.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8617 mL

9.3087 mL

18.6175 mL

5 mM

0.3723 mL

1.8617 mL

3.7235 mL

10 mM

0.1862 mL

0.9309 mL

1.8617 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yu B, et al. Protective effects of tenuigenin on Staphylococcus aureus-induced pneumonia in mice. Microb Pathog. 2017 Sep;110:385-389. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8617 mL	9.3087 mL	18.6175 mL	46.5437 mL
	5 mM	0.3723 mL	1.8617 mL	3.7235 mL	9.3087 mL
	10 mM	0.1862 mL	0.9309 mL	1.8617 mL	4.6544 mL
	15 mM	0.1241 mL	0.6206 mL	1.2412 mL	3.1029 mL
	20 mM	0.0931 mL	0.4654 mL	0.9309 mL	2.3272 mL
	25 mM	0.0745 mL	0.3723 mL	0.7447 mL	1.8617 mL
	30 mM	0.0621 mL	0.3103 mL	0.6206 mL	1.5515 mL
	40 mM	0.0465 mL	0.2327 mL	0.4654 mL	1.1636 mL
	50 mM	0.0372 mL	0.1862 mL	0.3723 mL	0.9309 mL
	60 mM	0.0310 mL	0.1551 mL	0.3103 mL	0.7757 mL
	80 mM	0.0233 mL	0.1164 mL	0.2327 mL	0.5818 mL
	100 mM	0.0186 mL	0.0931 mL	0.1862 mL	0.4654 mL

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Tenuigenin

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Publications Citing Use of MedChemExpress (MCE) Tenuigenin

All NF-κB Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Tenuigenin Related Classifications

Cat. No.: HY-N0802