4'-Hydroxychalcone
Based on 3 publication(s) in Google Scholar
4'-Hydroxychalcone is a chalcone isolated from licorice root, with hepatoprotective activity. 4'-Hydroxychalcone inhibits TNFα-induced NF-κB activation via proteasome inhibition. 4'-Hydroxychalcone induces a rapid potassium release from mitochondrial vesicles and causes deterioration of respiratory control and oxidative phosphorylation of isolated rat liver mitochondria.
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- Purity: 99.66%
- CAS No.: 2657-25-2
- 화학식: C15H12O2
- 분자량:224.25
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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Publications Citing Use of MedChemExpress (MCE) 4'-Hydroxychalcone
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Biological Activity
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Cell Line
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Type | Value | Description | References |
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| CCRF-CEM | IC50 |
14.7 μM
Compound: 1
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Concentration required to inhibit 50% growth of Human CEM T-lymphocytes cells
Concentration required to inhibit 50% growth of Human CEM T-lymphocytes cells
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[PMID: 9544201] |
| DLD-1 | EC50 |
34 μM
Compound: 6
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Cytotoxicity against human DLD-1 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
Cytotoxicity against human DLD-1 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
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[PMID: 36356534] |
| FHC | CC50 |
82 μM
Compound: 6
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Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
Cytotoxicity against human FHC cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
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[PMID: 36356534] |
| HCT-116 | EC50 |
20 μM
Compound: 6
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Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
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[PMID: 36356534] |
| HCT-116 | EC50 |
21 μM
Compound: 6
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Cytotoxicity against p53-/- human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
Cytotoxicity against p53-/- human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
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[PMID: 36356534] |
| HEK293 | IC50 |
>50 μM
Compound: 3
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Blockade of human K+ channel in HEK293 cells assessed as inhibition of outward delayed rectifying K+ current
Blockade of human K+ channel in HEK293 cells assessed as inhibition of outward delayed rectifying K+ current
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[PMID: 18032041] |
| HT-29 | EC50 |
30 μM
Compound: 6
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Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 48 hrs by AlamarBlue-based assay
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[PMID: 36356534] |
| L1210 | IC50 |
23.5 μM
Compound: 1
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Concentration required to inhibit 50% growth of L1210 leukemia cells
Concentration required to inhibit 50% growth of L1210 leukemia cells
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[PMID: 9544201] |
| P388 | IC50 |
3.12 μM
Compound: 1
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Inhibition of proliferation of murine P388 cells
Inhibition of proliferation of murine P388 cells
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[PMID: 9544201] |
| RBL-1 | IC50 |
230 μM
Compound: 1
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Inhibition of 5-lipoxygenase in rat RBL1 cells
Inhibition of 5-lipoxygenase in rat RBL1 cells
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10.1007/s00044-013-0745-7 |
| RBL-1 | IC50 |
230 μM
Compound: 1
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Inhibition of cyclooxygenase in rat RBL1 cells
Inhibition of cyclooxygenase in rat RBL1 cells
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10.1007/s00044-013-0745-7 |
| RBL-1 | IC50 |
230 μM
Compound: 2
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In vitro inhibition against 5-lipoxygenase in RBL-1 cells was determined
In vitro inhibition against 5-lipoxygenase in RBL-1 cells was determined
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[PMID: 8254620] |
| THP-1 | IC50 |
0.12 nM
Compound: 3a
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Inhibition of tissue factor in Homo sapiens (human) THP1-cells using factor 10a chromogenic substrate assessed as inhibition of LPS-iduced procoagulant activity incubated for 1 hr prior to LPS-challenge measured after 5 hr by spectrophotometric analysis
Inhibition of tissue factor in Homo sapiens (human) THP1-cells using factor 10a chromogenic substrate assessed as inhibition of LPS-iduced procoagulant activity incubated for 1 hr prior to LPS-challenge measured after 5 hr by spectrophotometric analysis
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10.1007/s00044-012-0330-5 |
4'-Hydroxychalcone (20-40 μM ; 2 hours) inhibits TNFα-induced (20 ng/mL; 6 hours) NF-kB pathway activation in a dose-dependent manner[3].
4'-Hydroxychalcone (0.1-25 μM ; 8 hours) inhibits proteasome activity in a dose-dependent manner but has no effect on IKK activity[3].
4'-Hydroxychalcone inhibits TNFα-dependent degradation of IkBα and subsequently prevents p50/p65 nuclear translocation leading to 4'-Hydroxychalcone-inhibited expression of NF-kB target genes[3].
4'-Hydroxychalcone affects cancer cell viability but has no significant effect on non-transformed cell viability[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:K562 cells, Jurkat cells, U937 cells, PBMCs
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Concentration:5 μM, 10 μM, 20 μM, 24 μM, 28 μM, 32 μM, 40 μM, 60 μM
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Incubation Time:24 hours
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Result:Affected cancer cell viability but has no significant effect on non-transformed cell viability.
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Cell Line:Jurkat cells
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Concentration:60 μM (followed by TNFα 20 ng/mL )
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Incubation Time:2 hours
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Result:Inhibited TNFα -dependent degradation of IkBα and prevents p50/p65 nuclear translocation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male albino mice(5-30 g)[2]
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Dosage:25 mg/kg, 50 mg/kg, 100 mg/kg
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Administration:Oral administration, 12h intervals, 4 doses
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Result:Significantly reduced the mortality rate induced by Acetaminophen (1 g/kg).
Chemical Information
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CAS No. 2657-25-2
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Appearance Solid
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분자량 224.25
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화학식 C15H12O2
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Color Light yellow to brown
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SMILES
O=C(C1=CC=C(O)C=C1)/C=C/C2=CC=CC=C2
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Structure Classification
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Food Chem
Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]2025 Dec 30:497:146992. PMID: 41285060 -
Food Chem
Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]2025 May 31:489:144992. PMID: 40466530 -
Phytother Res
4'-Hydroxychalcone Induces Ferroptosis in Glioblastoma Through the xCT/GSH/GPX4 Axis Under the Regulation of the AKT/mTORC1/4EBP1 Pathway. [Abstract]2025 Aug 25. PMID: 40855403
용액&용해도
DMSO : 233.33 mg/mL (1040.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Inoue B, et al. The effects of echinatin and its related compounds on the mitochondrial energy transfer reaction. J Toxicol Sci. 1982 Nov;7(4):245-54. [Content Brief]
[3]. Orlikova B, et al. The aromatic ketone 4'-hydroxychalcone inhibits TNFα-induced NF-κB activation via proteasome inhibition. Biochem Pharmacol. 2011 Sep 15;82(6):620-31. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.4593 mL | 22.2965 mL | 44.5931 mL | 111.4827 mL |
| 5 mM | 0.8919 mL | 4.4593 mL | 8.9186 mL | 22.2965 mL | |
| 10 mM | 0.4459 mL | 2.2297 mL | 4.4593 mL | 11.1483 mL | |
| 15 mM | 0.2973 mL | 1.4864 mL | 2.9729 mL | 7.4322 mL | |
| 20 mM | 0.2230 mL | 1.1148 mL | 2.2297 mL | 5.5741 mL | |
| 25 mM | 0.1784 mL | 0.8919 mL | 1.7837 mL | 4.4593 mL | |
| 30 mM | 0.1486 mL | 0.7432 mL | 1.4864 mL | 3.7161 mL | |
| 40 mM | 0.1115 mL | 0.5574 mL | 1.1148 mL | 2.7871 mL | |
| 50 mM | 0.0892 mL | 0.4459 mL | 0.8919 mL | 2.2297 mL | |
| 60 mM | 0.0743 mL | 0.3716 mL | 0.7432 mL | 1.8580 mL | |
| 80 mM | 0.0557 mL | 0.2787 mL | 0.5574 mL | 1.3935 mL | |
| 100 mM | 0.0446 mL | 0.2230 mL | 0.4459 mL | 1.1148 mL |
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