WAY-200070
Based on 1 publication(s) in Google Scholar
WAY-200070 is a selective estrogen receptor β (ERβ) agonist with an IC50 of 2.3 nM.
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- Purity: 99.57%
- CAS No.: 440122-66-7
- 화학식: C13H8BrNO3
- 분자량:306.11
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) WAY-200070
More
Biological Activity
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ERβ 2.3 nM (IC50) |
ERα 155 nM (IC50) |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 440122-66-7
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Appearance Solid
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분자량 306.11
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화학식 C13H8BrNO3
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Color White to off-white
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SMILES
OC1=CC(Br)=C(OC(C2=CC=C(O)C=C2)=N3)C3=C1
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (1)
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Journal Impact Factor
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Most Recent
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Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576
용액&용해도
DMSO : ≥ 31 mg/mL (101.27 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 10 mg/mL (32.67 mM); Clear solution
This protocol yields a clear solution of ≥ 10 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (100.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Rats: Beginning 1 wk after ovariectomy, animals are given a single daily sc injection of hydroxypropyl betacyclodextran [vehicle; 27% (wt/vol) in saline; DPN (2.0 mg/kg), S-DPN (2.0 mg/kg), R-DPN (2.0 mg/kg), WAY-200070-3 (2.0 mg/kg), or PPT (1.0 mg/kg) in a total volume of 0.2 mL. Three hours after the daily treatment injection on d 4-7, animals undergo behavioral testing[4].
Mice: WAY-200070 is dissolved in a 10% ethanol/90% miglyol solution. WAY-200070 or vehicle is injected subcutaneously at a volume of 10 mL/kg body weight. Male ERβKO, ERαKO (both in C57BL/6 background) and WT C57BL/6 mice are injected with vehicle or WAY-200070 (30 mg/kg s.c.). After 15 min, the animals are sacrificed and the striatum is dissected and quickly frozen in liquid nitrogen and stored at -70°C for subsequent assay[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
순도&문서
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Data Sheet (274 KB)
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SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
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Handling Instructions (2659 KB)
References
[1]. Hughes ZA, et al. WAY-200070, a selective agonist of estrogen receptor beta as a potential novel anxiolytic/antidepressant agent. Neuropharmacology. 2008 Jun;54(7):1136-42. [Content Brief]
[2]. Clipperton Allen AE, et al. Agonistic behavior in males and females: effects of an estrogen receptor beta agonist in gonadectomized and gonadally intact mice. Psychoneuroendocrinology. 2010 Aug;35(7):1008-22. [Content Brief]
[3]. Clipperton AE, et al. Differential effects of estrogen receptor alpha and beta specific agonists on social learning of food preferences in female mice. Neuropsychopharmacology. 2008 Sep;33(10):2362-75. [Content Brief]
[4]. Weiser MJ, et al. Estrogen receptor-beta agonist diarylpropionitrile: biological activities of R- and S-enantiomers on behavior and hormonal response to stress. Endocrinology. 2009 Apr;150(4):1817-25. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2668 mL | 16.3340 mL | 32.6680 mL | 81.6700 mL |
| 5 mM | 0.6534 mL | 3.2668 mL | 6.5336 mL | 16.3340 mL | |
| 10 mM | 0.3267 mL | 1.6334 mL | 3.2668 mL | 8.1670 mL | |
| 15 mM | 0.2178 mL | 1.0889 mL | 2.1779 mL | 5.4447 mL | |
| 20 mM | 0.1633 mL | 0.8167 mL | 1.6334 mL | 4.0835 mL | |
| 25 mM | 0.1307 mL | 0.6534 mL | 1.3067 mL | 3.2668 mL | |
| 30 mM | 0.1089 mL | 0.5445 mL | 1.0889 mL | 2.7223 mL | |
| 40 mM | 0.0817 mL | 0.4083 mL | 0.8167 mL | 2.0417 mL | |
| 50 mM | 0.0653 mL | 0.3267 mL | 0.6534 mL | 1.6334 mL | |
| 60 mM | 0.0544 mL | 0.2722 mL | 0.5445 mL | 1.3612 mL | |
| 80 mM | 0.0408 mL | 0.2042 mL | 0.4083 mL | 1.0209 mL | |
| 100 mM | 0.0327 mL | 0.1633 mL | 0.3267 mL | 0.8167 mL |
WAY-200070 Related Classifications
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