Cat. No.: HY-13550

Ametantrone

(Synonyms: NSC 196473; NSC 290813)

고객리뷰

Based on 1 Customer Validation

Ametantrone (NSC 196473) is an antitumor agent that intercalates into DNA and induces topoisomerase II (TOP2)-mediated DNA break.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 97.28%
CAS No.: 64862-96-0
화학식: C₂₂H₂₈N₄O₄
분자량:412.48

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Ametantrone Chemical Structure

CAS No.: 64862-96-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- Cellular Effect
Chemical Information
용액&용해도
- In Vitro
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

Ametantrone (NSC 196473) is an antitumor agent that intercalates into DNA and induces topoisomerase II (TOP2)-mediated DNA break^[1].

All DNA/RNA Synthesis Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	1108 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line A2780/Dx(RI) In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line A2780/Dx(RI)	[PMID: 10479282]
A2780	IC₅₀	180 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line A2780 In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line A2780	[PMID: 10479282]
DU-145	IC₅₀	1020 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line DU145 In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line DU145	[PMID: 10479282]
HeLa	IC₅₀	0.17 μg/mL Compound: ametantrone	In vitro cytotoxicity against HeLa S3 cell lines In vitro cytotoxicity against HeLa S3 cell lines	[PMID: 2213837]
HeLa	IC₅₀	0.9 μM Compound: Ametantrone	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 27521587]
HeLa	IC₅₀	0.9 μM Compound: Ametantrone	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 28342691]
HeLa	EC₅₀	0.17 μg/mL Compound: 2	Effective concentration required to inhibit by 50% the growth of HeLa S3 cells Effective concentration required to inhibit by 50% the growth of HeLa S3 cells	[PMID: 3172129]
HeLa	ED₅₀	3.1 mM Compound: AM	Effective concentration against cell growth of HeLa cell line by 50% Effective concentration against cell growth of HeLa cell line by 50%	[PMID: 8759632]
HL-60	IC₅₀	15.9 nM Compound: Ametantrone	Compound was tested in vitro for cytotoxicity against HL60 human leukemia cell line (72 hr exposure to compound) Compound was tested in vitro for cytotoxicity against HL60 human leukemia cell line (72 hr exposure to compound)	[PMID: 10479282]
HL-60	GI₅₀	29 nM Compound: AT, ametantrone	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 17962028]
HL-60	ED₅₀	0.88 mM Compound: AM	Effective concentration against cell growth of HL60 cell line by 50% Effective concentration against cell growth of HL60 cell line by 50%	[PMID: 8759632]
HL60/MX2	GI₅₀	430 nM Compound: AT, ametantrone	Cytotoxicity against DNA topoisomerase-2 deficient human HL60/MX2 cells by MTT assay Cytotoxicity against DNA topoisomerase-2 deficient human HL60/MX2 cells by MTT assay	[PMID: 17962028]
K562	IC₅₀	12392 nM Compound: Ametantrone	Compound was tested in vitro for cytotoxicity against MDR cell line K562R (RI) (72 hr exposure to compound) Compound was tested in vitro for cytotoxicity against MDR cell line K562R (RI) (72 hr exposure to compound)	[PMID: 10479282]
K562	IC₅₀	181 nM Compound: Ametantrone	Compound was tested in vitro for cytotoxicity against MDR cell line K562R (72 hr exposure to compound) Compound was tested in vitro for cytotoxicity against MDR cell line K562R (72 hr exposure to compound)	[PMID: 10479282]
L1210	IC₅₀	146 nM Compound: Ametantrone	Compound was tested in vitro for cytotoxicity against L1210 murine cell line (48 hr exposure to compound) Compound was tested in vitro for cytotoxicity against L1210 murine cell line (48 hr exposure to compound)	[PMID: 10479282]
L1210	IC₅₀	1.77 μg/mL Compound: ametantrone	In vitro inhibitory activity against murine L1210 leukemia In vitro inhibitory activity against murine L1210 leukemia	[PMID: 1732555]
L1210	IC₅₀	3.65 μM Compound: ametantrone	In vitro inhibitory activity against murine L1210 leukemia In vitro inhibitory activity against murine L1210 leukemia	[PMID: 1732555]
L1210	EC₅₀	0.21 g/mL Compound: ametantrone	Effective concentration against inhibition of L1210 mouse leukemia cells in tissue culture Effective concentration against inhibition of L1210 mouse leukemia cells in tissue culture	[PMID: 1995874]
L1210	ED₅₀	2.54 μg/mL Compound: 1 (ametantrone)	Concentration required to inhibit the growth of L1210 leukemia cells Concentration required to inhibit the growth of L1210 leukemia cells	[PMID: 2754697]
L1210	EC₅₀	1.77 μg/mL Compound: 2	Effective concentration required to inhibit by 50% the growth of L1210 cells Effective concentration required to inhibit by 50% the growth of L1210 cells	[PMID: 3172129]
L1210	IC₅₀	0.03 μg/mL Compound: 1a (Ametantrone)	Tested in vitro for inhibitory activity against L1210 murine leukemia Tested in vitro for inhibitory activity against L1210 murine leukemia	[PMID: 8145234]
L1210	EC₅₀	275 nM Compound: AME	Concentration of compound required to inhibit the cellular protein biosynthesis by 50% Concentration of compound required to inhibit the cellular protein biosynthesis by 50%	[PMID: 8421288]
L1210	ED₅₀	0.1 mM Compound: AM	Effective concentration against cell growth of L1210 cell line by 50% Effective concentration against cell growth of L1210 cell line by 50%	[PMID: 8759632]
LoVo	IC₅₀	36802 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line LoVo/DX(RI) In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line LoVo/DX(RI)	[PMID: 10479282]
LoVo	IC₅₀	641 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line LoVo In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line LoVo	[PMID: 10479282]
LoVo	IC₅₀	5.8 x 10^-7 M Compound: Ametantrone	Inhibitory activity against human colon adenocarcinoma cell line LoVo. Inhibitory activity against human colon adenocarcinoma cell line LoVo.	[PMID: 7853345]
LoVo	IC₅₀	9.2 x 10^-5 M Compound: Ametantrone	Inhibitory activity against human colon adenocarcinoma cell line LoVo/Dx. Inhibitory activity against human colon adenocarcinoma cell line LoVo/Dx.	[PMID: 7853345]
LoVo	IC₅₀	0.4 μg/mL Compound: 1a (Ametantrone)	Tested in vitro for cytotoxic activity against Human colon Adenocarcinoma sensitive cell line (LoVo) Tested in vitro for cytotoxic activity against Human colon Adenocarcinoma sensitive cell line (LoVo)	[PMID: 8145234]
LoVo	IC₅₀	34.7 μg/mL Compound: 1a (Ametantrone)	Tested in vitro for cytotoxic activity against Doxorubicin resistant Human colon Adenocarcinoma sensitive cell line Tested in vitro for cytotoxic activity against Doxorubicin resistant Human colon Adenocarcinoma sensitive cell line	[PMID: 8145234]
MCF7	GI₅₀	4.97 μM Compound: AT, ametantrone	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay	[PMID: 17962028]
MCF7	IC₅₀	0.51 μM Compound: Ametantrone	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 27521587]
MCF7	IC₅₀	0.51 μM Compound: Ametantrone	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 28342691]
NCI-H460	GI₅₀	2.55 μM Compound: AT, ametantrone	Cytotoxicity against human NCI-H460 cells after 48 hrs by SRB assay Cytotoxicity against human NCI-H460 cells after 48 hrs by SRB assay	[PMID: 17962028]
PC-3	IC₅₀	1848 nM Compound: Ametantrone	In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line PC3 In vitro Cytotoxic activity of compound in comparison with reference compounds in human cell line PC3	[PMID: 10479282]
PC-3	GI₅₀	8.52 μM Compound: AT, ametantrone	Cytotoxicity against human PC3 cells after 48 hrs by SRB assay Cytotoxicity against human PC3 cells after 48 hrs by SRB assay	[PMID: 17962028]
SF-268	GI₅₀	2.45 μM Compound: AT, ametantrone	Cytotoxicity against human SF268 cells after 48 hrs by SRB assay Cytotoxicity against human SF268 cells after 48 hrs by SRB assay	[PMID: 17962028]

Chemical Information

CAS No. 64862-96-0
Appearance Solid
분자량 412.48
화학식 C₂₂H₂₈N₄O₄
Color Brown to black
SMILES

O=C1C2=C(C=CC=C2)C(C3=C(NCCNCCO)C=CC(NCCNCCO)=C13)=O
Synonyms

NSC 196473; NSC 290813
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 1.67 mg/mL (4.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4243 mL

12.1217 mL

24.2435 mL

5 mM

---

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (266 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lee CH, et al. Anthracenedione-methionine conjugates are novel topoisomerase II-targeting anticancer agents with favorable drug resistance profiles. Biochem Pharmacol. 2012;83(9):1208-1216. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4243 mL	12.1217 mL	24.2435 mL	60.6087 mL

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Ametantrone

Human Metabolite Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Ametantrone

Human Metabolite Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Cell Cycle/DNA Damage Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Ametantrone Related Classifications

HY-13550 Related Classifications