L-NIL hydrochloride
Based on 10 publication(s) in Google Scholar
L-NIL hydrochloride is an inducible NO synthase inhibitor, with an IC50 of 3.3 μM for mouse inducible NOS.
For research use only. We do not sell to patients.
- CAS No.: 150403-89-7
- Formula: C8H18ClN3O2
- Molecular Weight:223.70
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) L-NIL hydrochloride
More- Nat Biomed Eng. 2023 Mar;7(3):281-297. [Abstract]
- Nat Commun. 2025 Jul 1;16(1):5547. [Abstract]
- Sci Transl Med. 2025 May 21;17(799):eadn7527. [Abstract]
- J Adv Res. 2023 Jan:43:175-186. [Abstract]
- Cancer Lett. 2023 Aug 28:570:216330. [Abstract]
- Free Radic Biol Med. 2023 May 1:200:73-86. [Abstract]
- J Agric Food Chem. 2024 May 16. [Abstract]
- Exp Eye Res. 2024 Jun:243:109886. [Abstract]
- Biochem Biophys Res Commun. 2024 Aug 22:734:150589. [Abstract]
- bioRxiv. 2026 Jun 13:2026.06.12.731980. [Abstract]
Customer Validation & Images
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WB
Biological Activity
IC50: 3.3 μM (mouse inducible NO synthase), 92 μM (rat brain constitutive NO synthase)[1].
L-NIL produces a concentration-dependent inhibition of both the mouse inducible NOS and the rat brain constitutive NOS (rcNOS) and is considerably more potent for mouse inducible NOS. The IC50 values for L-NIL with mouse inducible NOS and rcNOS are 3.3 and 92 pM, respectively, indicating that L-NIL is 28-fold more selective for mouse inducible NOS. In addition, L-NIL has approximately 6-fold greater potency for mouse inducible NOS than either L-NMA or L-NNA[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult male Balb/c (20-25 g)[1].
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Dosage:10 and 30 mg/kg.
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Administration:Intraperitoneally at the end of CLP and at 6 h after sepsis induction.
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Result:Led to a negligible increase in plasma NGAL compared to sham mice.
Led to a significant decrease in both TLR4 and IL1βprotein contents and clusterin transcript.
Showed an increase in NFAT5 mRNA levels, as compared with mice treated with vehicle.
Promoted a decrease in AR protein expression, as compared with animals treated with vehicle.
Chemical Information
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CAS No. 150403-89-7
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Molecular Weight 223.70
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Formula C8H18ClN3O2
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SMILES
OC([C@@H](N)CCCCNC(C)=N)=O.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (10)
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Journal Impact Factor
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Most Recent
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Nat Biomed Eng
Activatable near-infrared probes for the detection of specific populations of tumour-infiltrating leukocytes in vivo and in urine. [Abstract]2023 Mar;7(3):281-297. PMID: 36941352 -
Nat Commun
Host glutathione is required for Rickettsia parkeri cell division and intracellular survival. [Abstract]2025 Jul 1;16(1):5547. PMID: 40593553 -
Sci Transl Med
SARS-CoV-2 induces neutrophil degranulation and differentiation into myeloid-derived suppressor cells associated with severe COVID-19. [Abstract]2025 May 21;17(799):eadn7527. PMID: 40397714 -
J Adv Res
iNOS contributes to heart failure with preserved ejection fraction through mitochondrial dysfunction and Akt S-nitrosylation. [Abstract]2023 Jan:43:175-186. PMID: 36585107
L-NIL hydrochloride purchased from MedChemExpress. Usage Cited in: J Adv Res. 2023 Jan:43:175-186. [Abstract]
Inhibiting iNOS with L-NIL (10 μM) blunted the adverse effect of iNOS expression in cardiomyocytes.
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Cancer Lett
Inducible nitric oxide synthase (iNOS)-activated Cxcr2 signaling in myeloid cells promotes TGFβ-dependent squamous cell carcinoma lung metastasis. [Abstract]2023 Aug 28:570:216330. PMID: 37524225 -
Free Radic Biol Med
Cigarette smoke induces the ROS accumulation and iNOS activation through deactivation of Nrf-2/SIRT3 axis to mediate the human bronchial epithelium ferroptosis. [Abstract]2023 May 1:200:73-86. PMID: 36871899 -
J Agric Food Chem
Quercetin Inhibits Neuronal Pyroptosis and Ferroptosis by Modulating Microglial M1/M2 Polarization in Atherosclerosis. [Abstract]2024 May 16. PMID: 38755521 -
Exp Eye Res
2024 Jun:243:109886. PMID: 38583755 -
Biochem Biophys Res Commun
Exploring the role of iNOS in HFpEF-Related myocardial fibrosis: Involvement of PTEN-PI3K/AKT signaling pathway. [Abstract]2024 Aug 22:734:150589. PMID: 39245028 -
bioRxiv
A genetic screen identifies O-antigen as essential for Rickettsia parkeri survival in macrophages by protecting from inflammasomes and interferon-stimulated genes. [Abstract]2026 Jun 13:2026.06.12.731980. PMID: 42327330
Solvent & Solubility
DMSO : 100 mg/mL (447.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (11.18 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (11.18 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References
[1]. Consuelo Pasten, et al. l-NIL prevents the ischemia and reperfusion injury involving TLR-4, GST, clusterin, and NFAT-5 in mice. Am J Physiol Renal Physiol. 2019 Apr 1;316(4):F624-F634. [Content Brief]
[2]. Sharon Angela Tanuseputero, et al. Intravenous Arginine Administration Downregulates NLRP3 Inflammasome Activity and Attenuates Acute Kidney Injury in Mice with Polymicrobial Sepsis. Mediators Inflamm. 2020 May 11;2020:3201635. [Content Brief]
[3]. Moore WM, et al. L-N6-(1-iminoethyl)lysine: a selective inhibitor of inducible nitric oxide synthase. J Med Chem. 1994 Nov 11;37(23):3886-8. [Content Brief]
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.4703 mL | 22.3514 mL | 44.7027 mL | 111.7568 mL |
| 5 mM | 0.8941 mL | 4.4703 mL | 8.9405 mL | 22.3514 mL | |
| 10 mM | 0.4470 mL | 2.2351 mL | 4.4703 mL | 11.1757 mL | |
| 15 mM | 0.2980 mL | 1.4901 mL | 2.9802 mL | 7.4505 mL | |
| 20 mM | 0.2235 mL | 1.1176 mL | 2.2351 mL | 5.5878 mL | |
| 25 mM | 0.1788 mL | 0.8941 mL | 1.7881 mL | 4.4703 mL | |
| 30 mM | 0.1490 mL | 0.7450 mL | 1.4901 mL | 3.7252 mL | |
| 40 mM | 0.1118 mL | 0.5588 mL | 1.1176 mL | 2.7939 mL | |
| 50 mM | 0.0894 mL | 0.4470 mL | 0.8941 mL | 2.2351 mL | |
| 60 mM | 0.0745 mL | 0.3725 mL | 0.7450 mL | 1.8626 mL | |
| 80 mM | 0.0559 mL | 0.2794 mL | 0.5588 mL | 1.3970 mL | |
| 100 mM | 0.0447 mL | 0.2235 mL | 0.4470 mL | 1.1176 mL |
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