Metronidazole Benzoate
Based on 1 Customer Validation
Metronidazole Benzoate, derives from a metronidazole and a benzoic acid, has a role as an antibacterial, antimicrobial, antiparasitic, and antitrichomonal agent.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 13182-89-3
- Formula: C13H13N3O4
- Molecular Weight:275.26
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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FDA Approved & Pharmacopeial Drug Library
HY-L066
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Antiparasitic Compound library
HY-L082
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Human Metabolite Library
HY-L123
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HY-L141
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HY-L181
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HY-L001V
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HY-L035P
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HY-L022P
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HY-L022M
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HY-L001
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HY-L205
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HY-L215
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HY-L907
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HY-L002
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HY-L022
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HY-L035
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Antibacterial Compound Library
HY-L049
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NMPA-Approved Drug Library
HY-L053
All Parasite Isoforms
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Biological Activity
Antibacterial, antimicrobial, antiparasitic, antitrichomonal[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
280.9 μg/mL
Compound: 4n
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Cytotoxicity against human A549 cells after 24 hrs by MTT assay
Cytotoxicity against human A549 cells after 24 hrs by MTT assay
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[PMID: 30554970] |
| HT-29 | IC50 |
287.67 μg/mL
Compound: 4n
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Cytotoxicity against human HT-29 cells after 24 hrs by MTT assay
Cytotoxicity against human HT-29 cells after 24 hrs by MTT assay
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[PMID: 30554970] |
| MCF7 | IC50 |
244.65 μg/mL
Compound: 4n
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Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay
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[PMID: 30554970] |
Metronidazole Benzoate is indicated for the treatment of infections caused by a wide range of anaerobic, protozoan and bacteroid bacteria, including trichomoniasis, amebiasis, vaginosis and gingivitis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 13182-89-3
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Appearance Solid
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Molecular Weight 275.26
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Formula C13H13N3O4
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Color White to off-white
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SMILES
O=[N+](C1=CN=C(C)N1CCOC(C2=CC=CC=C2)=O)[O-]
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Synonyms
Benzoyl metronidazole
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 250 mg/mL (908.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6329 mL | 18.1646 mL | 36.3293 mL | 90.8232 mL |
| 5 mM | 0.7266 mL | 3.6329 mL | 7.2659 mL | 18.1646 mL | |
| 10 mM | 0.3633 mL | 1.8165 mL | 3.6329 mL | 9.0823 mL | |
| 15 mM | 0.2422 mL | 1.2110 mL | 2.4220 mL | 6.0549 mL | |
| 20 mM | 0.1816 mL | 0.9082 mL | 1.8165 mL | 4.5412 mL | |
| 25 mM | 0.1453 mL | 0.7266 mL | 1.4532 mL | 3.6329 mL | |
| 30 mM | 0.1211 mL | 0.6055 mL | 1.2110 mL | 3.0274 mL | |
| 40 mM | 0.0908 mL | 0.4541 mL | 0.9082 mL | 2.2706 mL | |
| 50 mM | 0.0727 mL | 0.3633 mL | 0.7266 mL | 1.8165 mL | |
| 60 mM | 0.0605 mL | 0.3027 mL | 0.6055 mL | 1.5137 mL | |
| 80 mM | 0.0454 mL | 0.2271 mL | 0.4541 mL | 1.1353 mL | |
| 100 mM | 0.0363 mL | 0.1816 mL | 0.3633 mL | 0.9082 mL |
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